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Characterization of a member of a new Potyvirus species infecting arracacha in Brazil

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Abstract

An arracacha (Arracacia xanthorrhiza) plant collected in Brazil was found to be infected by a new virus. This viral isolate (named C17) systemically infected Nicotiana benthamiana and Apium graveolens. A polyclonal antibody was raised, and analysis of our arracacha germplasm collection showed a high infection rate of C17-like viruses (93% infection). Sequencing of the ca. 1.7 kb 3′-terminal genomic region revealed a typical potyvirus genome organization. It shared less than 70% nucleotide identity with any other potyvirus sequence, which thus indicated that it is possibly a member of a new Potyvirus species tentatively named Arracacha mottle virus (AMoV).

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Acknowledgments

We are indebted to Élcio Vieira da Silva Lopes (in memorian), who assisted us with cloning of the 3′ genomic fragment, and Bergmann Morais Ribeiro and Guy de Capedevile for assistance in electron microscopy.

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Authors and Affiliations

  1. University of Brasília, Campus Universitário, CEP 70.910-900, Brasilia, DF, Brazil

    A. F. Orílio & Alice Kazuko Inoue-Nagata

  2. Embrapa Vegetables, Caixa Postal 218, CEP 70.359-970, Brasilia, DF, Brazil

    A. F. Orílio, A. N. Dusi, N. R. Madeira & Alice Kazuko Inoue-Nagata

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  1. A. F. Orílio
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  2. A. N. Dusi
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  3. N. R. Madeira
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  4. Alice Kazuko Inoue-Nagata
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Correspondence to Alice Kazuko Inoue-Nagata.

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Orílio, A.F., Dusi, A.N., Madeira, N.R. et al. Characterization of a member of a new Potyvirus species infecting arracacha in Brazil. Arch Virol 154, 181–185 (2009). https://doi.org/10.1007/s00705-008-0289-1

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