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Nucleotide sequence and genome organization of a member of a new and distinct Caulimovirus species from dahlia

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Abstract

A distinct caulimovirus, associated with dahlia mosaic, was cloned and sequenced. The caulimovirus, tentatively designated as dahlia common mosaic virus (DCMV), had a double-stranded DNA genome of ca. 8 kb. The genome organization of DCMV was found to be typical of members of the genus Caulimovirus and consisted of six major open reading frames (ORFs), ORFs I–VI, and one minor ORF, ORF VII. Sequence comparisons with the DNA genomes of two known caulimoviruses isolated from dahlia, Dahlia mosaic virus (DMV) and an endogenous caulimovirus, DMV-D10, showed that DCMV is a member of a distinct caulimovirus species, with sequence identities among various ORFs ranging from 25 to 80%.

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Acknowledgments

Research was supported in part by the Samuel and Patricia Smith Endowment for Dahlia Virus Research, created by the American Dahlia Society, and a grant from the USDA ARS Northwest Nursery Crop Research Center. PPNS No. 0506, Department of Plant Pathology, College of Agricultural, Human and Natural Resource Sciences, Agricultural Research Center, Project # WNPO 0545, Washington State University, Pullman, WA 99164-6430, USA.

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Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, PO Box 646430, Pullman, WA, 99164-6430, USA

    H. R. Pappu & K. L. Druffel

  2. Dutch Flower Bulb Inspection Service, PO Box 300, 2160 AH, Lisse, The Netherlands

    R. Miglino & A. R. van Schadewijk

Authors
  1. H. R. Pappu
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  2. K. L. Druffel
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  3. R. Miglino
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  4. A. R. van Schadewijk
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Corresponding author

Correspondence to H. R. Pappu.

Additional information

Sequences reported here are available in GenBank under the following accessions: EU090952, EU090953, EU090954, EU090955, EU090956, EU090957.

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Pappu, H.R., Druffel, K.L., Miglino, R. et al. Nucleotide sequence and genome organization of a member of a new and distinct Caulimovirus species from dahlia. Arch Virol 153, 2145–2148 (2008). https://doi.org/10.1007/s00705-008-0235-2

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  • DOI: https://doi.org/10.1007/s00705-008-0235-2

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