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Archives of Virology

, 153:2123 | Cite as

Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

  • Andor Doszpoly
  • Endre R. Kovács
  • Giuseppe Bovo
  • Scott E. LaPatra
  • Balázs Harrach
  • Mária Benkő
Brief Report

Abstract

A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon (Acipenser transmontanus) and black bullhead (Ameiurus melas) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictalurivirus in the new family Alloherpesviridae.

Keywords

White Sturgeon Phylogenetic Tree Reconstruction Black Bullhead Acipenser Transmontanus Restriction Enzyme Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was supported by a grant (OTKA K61317) provided by the Hungarian Scientific Research Fund.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Andor Doszpoly
    • 1
  • Endre R. Kovács
    • 1
  • Giuseppe Bovo
    • 2
  • Scott E. LaPatra
    • 3
  • Balázs Harrach
    • 1
  • Mária Benkő
    • 1
  1. 1.Veterinary Medical Research Institute, Hungarian Academy of SciencesBudapestHungary
  2. 2.Istituto Zooprofilattico Sperimentale delle VenezieLegnaroItaly
  3. 3.Research DivisionClear Springs Foods Inc.BuhlUSA

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