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The C-terminal region of envelope protein VP38 from white spot syndrome virus is indispensable for interaction with VP24

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Abstract

White spot syndrome virus (WSSV) is a large, rod-shaped, enveloped double-stranded DNA virus. In this study, VP38, a viral envelope protein, was expressed as a glutathione S-transferase (GST) fusion protein, and a polyclonal antibody against VP38 was obtained. Far-Western blotting and GST pull-down showed that VP38 interacted directly with VP24, a major WSSV envelope protein. In addition, to delineate the interaction region of VP38 with VP24, GST-VP38n (aa 1–142) and GST-VP38c (aa 143–309) were expressed. The GST pull-down assay revealed that VP38 binds via its C-terminal region to VP24. The result implies that VP38 may participate in the formation of the WSSV envelope.

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Acknowledgments

This investigation was supported by Natural Science Foundation of China (3077164035), National “863” Program of China (2006AA100312 and 2006AA09Z445) and National “973” Program of China (2006CB101801).

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Authors and Affiliations

  1. School of Life Sciences, Xiamen University, 361005, Xiamen, People’s Republic of China

    Zuliang Jie

  2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, 178 Daxue Rd., 361005, Xiamen, People’s Republic of China

    Limei Xu & Feng Yang

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  1. Zuliang Jie
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  2. Limei Xu
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  3. Feng Yang
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Correspondence to Feng Yang.

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Jie, Z., Xu, L. & Yang, F. The C-terminal region of envelope protein VP38 from white spot syndrome virus is indispensable for interaction with VP24. Arch Virol 153, 2103–2106 (2008). https://doi.org/10.1007/s00705-008-0221-8

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  • DOI: https://doi.org/10.1007/s00705-008-0221-8

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