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Phylogenetic analysis of rotaviruses with genotypes G1, G2, G9 and G12 in Bangladesh: evidence for a close relationship between rotaviruses from children and adults

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Abstract

To clarify the phylogenetic relatedness of rotaviruses causing gastroenteritis in children and adults, an epidemiologic investigation was conducted in Mymensingh, Bangladesh, during the period between July 2004 and June 2006. A total of 2,540 stool specimens from diarrheal patients from three hospitals were analyzed. Overall, rotavirus-positive rates in children and adults were 26.4 and 10.1%, respectively. Among the 155 rotavirus specimens examined genetically from both children and adults, the most frequent G genotype was G2 (detection rate: 54.0 and 47.6%, respectively), followed by G1 (21.2 and 26.2%, respectively), and G9 (15.9 and 9.5%, respectively). G12 was also detected in five specimens (3.2% in total; four children and one adult). Sequence identities of VP7 genes of G2 rotaviruses from children and adults were higher than 97.8%, while these Bangladeshi G2 viruses showed generally lower identities to G2 rotaviruses reported elsewhere in the world, except for some strains reported in African countries. Similarly, extremely high sequence identities between children and adults were observed for VP7 genes of G1, G9 and G12 rotaviruses, and also for the VP4 genes of P[4], P[6], and P[8] viruses. Rotaviruses from children and adults detected in this study were included in a single cluster in phylogenetic dendrograms of VP7 or VP4 genes of individual G/P types. Rotaviruses with two emerging types, G9 and G12, had VP7 genes that were phylogenetically close to those of individual G-types recently reported in Bangladesh and India and were included in the globally spreading lineages of these G-types. These findings suggested that genetically identical rotaviruses, including those with the emerging types G9 and G12, were circulating among children and adults in city and rural areas of Bangladesh.

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References

  1. Ahmed MU, Taniguchi K, Kobayashi N, Urasawa T, Wakasugi F, Islam M, Shaikh H, Urasawa S (1989) Characterization by enzyme-linked immunosorbent assay using subgroup and serotype-specific monoclonal antibodies of human rotavirus obtained from diarrheic patients in Bangladesh. J Clin Microbiol 27:1678–1681

    PubMed  CAS  Google Scholar 

  2. Ahmed MU, Urasawa S, Taniguchi K, Urasawa T, Kobayashi N, Wakasugi F, Islam AI, Sahikh HA (1991) Analysis of human rotavirus strains prevailing in Bangladesh in relation to nationwide floods brought by the 1988 monsoon. J Clin Microbiol 29:2273–2279

    PubMed  CAS  Google Scholar 

  3. Alam MM, Kobayashi N, Ishino M, Ahmed MS, Ahmed MU, Paul SK, Muzumdar BK, Hussain Z, Wang YH, Naik TN (2007) Genetic analysis of an ADRV-N-like novel rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh. Arch Virol 152:199–208

    Article  PubMed  CAS  Google Scholar 

  4. Anderson EJ, Weber SG (2004)Rotavirus infection in adults. Lancet Infect Dis 4:91–99

    Google Scholar 

  5. Barnes GL, Callaghan SL, Kirkwood CD, Bogdanovic-Sakran N, Johnston LJ, Bishop RF (2003) Excretion of serotype G1 rotavirus strains by asymptomatic staff: a possible source of nosocomial infection. J Pediatr 142:722–725

    Article  PubMed  Google Scholar 

  6. Bingnan F, Unicomb L, Rahim Z, Banu NN, Podder G, Clemens J, Van Loon FP, Rao MR, Malek A, Tzipori S (1991) Rotavirus-associated diarrhea in rural Bangladesh: two-year study of incidence and serotype distribution. J Clin Microbiol 29:1359–1363

    Google Scholar 

  7. Carraro E, Perosa AH, Siqueira I, Pasternak J, Martino MD (2008) Rotavirus infection in children and adult patients attending in a tertiary Hospital of São Paulo, Brazil. Braz J Infect Dis 12:44–46

    Article  PubMed  Google Scholar 

  8. Dennehy PH (2008) Rotavirus vaccines: an overview. Clin Microbiol Rev 21:198–208

    Article  PubMed  CAS  Google Scholar 

  9. Endara P, Trueba G, Solberg OD, Bates SJ, Ponce K, Cevallos W, Matthijnssens J, Eisenberg JN (2007) Symptomatic and subclinical infection with rotavirus P[8]G9, rural Ecuador. Emerg Infect Dis 13:574–580

    PubMed  CAS  Google Scholar 

  10. Estes MK, Kapikian AZ (2007) Rotaviruses. In: Knipe DM, Howley PM et al (eds) Fields Virology, 5th edn edn. Lippincott Williams & Wilkins, Philadelphia, PA, pp 1917–1974

    Google Scholar 

  11. Gentsch JR, Glass RI, Woods P, Gouvea V, Gorziglia M, Flores J, Das BK, Bhan MK (1992) Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30:1365–1373

    PubMed  CAS  Google Scholar 

  12. Griffin DD, Fletcher M, Levy ME, Ching-Lee M, Nogami R, Edwards L, Peters H, Montague L, Gentsch JR, Glass RI (2002) Outbreaks of adult gastroenteritis traced to a single genotype of rotavirus. J Infect Dis 185:1502–1505

    Article  PubMed  Google Scholar 

  13. Gurgel RQ, Cuevas LE, Vieira SC, Barros VC, Fontes PB, Salustino EF, Nakagomi O, Nakagomi T, Dove W, Cunliffe N, Hart CA (2007) Predominance of rotavirus P[4]G2 in a vaccinated population, Brazil. Emerg Infect Dis 13:1571–1573

    PubMed  Google Scholar 

  14. Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16:473–477

    PubMed  CAS  Google Scholar 

  15. Hrdy DB (1987) Epidemiology of rotaviral infection in adults. Rev Infect Dis 9:461–469

    PubMed  CAS  Google Scholar 

  16. Iijima Y, Iwamoto T, Nukuzuma S, Ohishi H, Hayashi K, Kobayashi N (2006) An outbreak of rotavirus infection among adults in an institution for rehabilitation: long-term residence in a closed community as a risk factor for rotavirus illness. Scand J Infect Dis 38:490–496

    Article  PubMed  Google Scholar 

  17. Iturriza-Gomara M, Green J, Brown DW, Ramsay M, Desselberger U, Gray JJ (2000) Molecular epidemiology of human group A rotavirus infections in the United Kingdom between 1995 and 1998. J Clin Microbiol 38:4394–4401

    PubMed  CAS  Google Scholar 

  18. Khamrin P, Peerakome S, Tonusin S, Malasao R, Okitsu S, Mizuguchi M, Ushijima H, Maneekarn N (2007) Changing pattern of rotavirus G genotype distribution in Chiang Mai, Thailand from 2002 to 2004: decline of G9 and reemergence of G1 and G2. J Med Virol 79:1775–1782

    Article  PubMed  CAS  Google Scholar 

  19. Kobayashi N, Lintag IC, Urasawa T, Taniguchi K, Saniel MC, Urasawa S (1989) Unusual human rotavirus strains having subgroup I specificity and “long” RNA electropherotype. Arch Virol 109:11–23

    Article  PubMed  CAS  Google Scholar 

  20. Nakajima H, Nakagomi T, Kamisawa T, Sakaki N, Muramoto K, Mikami T, Nara H, Nakagomi O (2001) Winter seasonality and rotavirus diarrhoea in adults. Lancet 357:1950

    Article  PubMed  CAS  Google Scholar 

  21. Noel JS, Beards GM, Cubitt WD (1991) Epidemiological survey of human rotavirus serotypes and electropherotypes in young children admitted to two children’s hospitals in northeast London from 1984 to 1990. J Clin Microbiol 29:2213–2219

    PubMed  CAS  Google Scholar 

  22. Page NA, Steele AD (2004) Antigenic and genetic characterization of serotype G2 human rotavirus strains from the African continent. J Clin Microbiol 42:595–600

    Article  PubMed  CAS  Google Scholar 

  23. Page NA, Steele AD (2004) Antigenic and genetic characterization of serotype G2 human rotavirus strains from South Africa from 1984 to 1998. J Med Virol 72:320–327

    Article  PubMed  CAS  Google Scholar 

  24. Parashar UD, Gibson CJ, Bresse JS, Glass RI (2006) Rotavirus and severe childhood diarrhea. Emerg Infect Dis 12:304–306

    PubMed  Google Scholar 

  25. Pietruchinski E, Benati F, Lauretti F, Kisielius J, Ueda M, Volotao EM, Soares CC, Hoshino Y, Linhares RE, Nozawa C, Santos N (2006) Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: distribution of G/P types and finding of a rare G12 strain. J Med Virol 78:1241–1249

    Article  PubMed  CAS  Google Scholar 

  26. Rahman M, Sultana R, Ahmed G, Nahar S, Hassan ZM, Saiada F, Podder G, Faruque AS, Siddique AK, Sack DA, Matthijnssens J, Van Ranst M, Azim T (2007) Prevalence of G2P[4] and G12P[6] rotavirus, Bangladesh. Emerg Infect Dis 13:18–24

    PubMed  CAS  Google Scholar 

  27. Rahman M, Matthijnssens J, Yang X, Delbeke T, Arijs I, Taniguchi K, Iturriza-Gómara M, Iftekharuddin N, Azim T, Van Ranst M (2007) Evolutionary history and global spread of the emerging G12 human rotaviruses. J Virol 81:2382–2390

    Article  PubMed  CAS  Google Scholar 

  28. Ramachandran M, Kirkwood CD, Unicomb L, Cunliffe NA, Ward RL, Bhan MK, Clark HF, Glass RI, Gentsch JR (2000) Molecular characterization of serotype G9 rotavirus strains from a global collection. Virology 278:436–444

    Article  PubMed  CAS  Google Scholar 

  29. Samajdar S, Varghese V, Barman P, Ghosh S, Mitra U, Dutta P, Bhattacharya SK, Narasimham MV, Panda P, Krishnan T, Kobayashi N, Naik TN (2006) Changing pattern of human group A rotaviruses: emergence of G12 as an important pathogen among children in eastern India. J Clin Virol 36:183–188

    Article  PubMed  CAS  Google Scholar 

  30. Santos N, Hoshino Y (2005) Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15:29–56

    Article  PubMed  Google Scholar 

  31. Sharma S, Ray P, Gentsch JR, Glass RI, Kalra V, Bhan MK (2008) Emergence of G12 rotavirus strains in Delhi, India, in 2000 to 2007. J Clin Microbiol 46:1343–1348

    Article  PubMed  CAS  Google Scholar 

  32. Steele AD, Peenze I, de Beer MC, Pager CT, Yeats J, Potgieter N, Ramsaroop U, Page NA, Mitchell JO, Geyer A, Bos P, Alexander JJ (2003) Anticipating rotavirus vaccines: epidemiology and surveillance of rotavirus in South Africa. Vaccine 21:354–360

    Article  PubMed  CAS  Google Scholar 

  33. Svenungsson B, Lagergren A, Ekwall E, Evengard B, Hedlund KO, Karnell A, Lofdahl S, Svensson L, Weintraub A (2000) Enteropathogens in adult patients with diarrhea and healthy control subjects: a 1-year prospective study in a Swedish clinic for infectious diseases. Clin Infect Dis 30:770–778

    Article  PubMed  CAS  Google Scholar 

  34. Taniguchi K, Wakasugi F, Pongsuwanna Y, Urasawa T, Ukae S, Chiba S, Urasawa S (1992) Identification of human and bovine rotavirus serotypes by polymerase chain reaction. Epidemiol Infect 109:303–312

    Article  PubMed  CAS  Google Scholar 

  35. Timenetsky MC, Gouvea V, Santos N, Alge ME, Kisiellius JJ, Carmona RC (1996) Outbreak of severe gastroenteritis in adults and children associated with type G2 rotavirus. J Diarrhoeal Dis Res 14:71–74

    PubMed  CAS  Google Scholar 

  36. Unicomb LE, Kilgore PE, Faruque SG, Hamadani JD, Fuchs GJ, Albert MJ, Glass RI (1997) Anticipating rotavirus vaccines: hospital-based surveillance for rotavirus diarrhea and estimates of disease burden in Bangladesh. Pediatr Infect Dis J 16:947–951

    Article  PubMed  CAS  Google Scholar 

  37. Unicomb LE, Podder G, Gentsch JR, Woods PA, Hasan KZ, Faruque AS, Albert MJ, Glass RI (1999) Evidence of high-frequency genomic reassortment of group A rotavirus strains in Bangladesh: emergence of type G9 in 1995. J Clin Microbiol 37:1885–1891

    PubMed  CAS  Google Scholar 

  38. Wang YH, Kobayashi N, Zhou DJ, Yang ZQ, Zhou X, Peng JS, Zhu ZR, Zhao DF, Liu MQ, Gong J (2007) Molecular epidemiologic analysis of group A rotaviruses in adults and children with diarrhea in Wuhan city, China, 2000–2006. Arch Virol 152:669–685

    Article  PubMed  CAS  Google Scholar 

  39. World Health Organization (2007) Rotavirus vaccines. Wkly Epidemiol Rec 82:285–296

    Google Scholar 

  40. Wu H, Taniguchi K, Urasawa T, Urasawa S (1998) Serological and genomic characterization of human rotaviruses detected in China. J Med Virol 55:168–176

    Article  PubMed  CAS  Google Scholar 

  41. Zao CL, Yu WN, Kao CL, Taniguchi K, Lee CY, Lee CN (1999) Sequence analysis of VP1 and VP7 genes suggests occurrence of a reassortant of G2 rotavirus responsible for an epidemic of gastroenteritis. J Gen Virol 80:1407–1415

    PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research (Grant no. 18406018) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and a Grant for International Health Cooperation Research (No. 18-5) from the Ministry of Health, Labour, and Welfare of Japan. Additionally, this work was supported in part by the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases (Okayama University; National Institute of Cholera and Enteric Diseases, India) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and Grant-in-Aid of the Japan Medical Association.

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Authors and Affiliations

  1. Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh

    Shyamal Kumar Paul & Mohammad Akram Hossain

  2. Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, 060-8556, Japan

    Nobumichi Kobayashi, Shigeo Nagashima, Masaho Ishino & Shojiro Watanabe

  3. Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh

    Mohammed Mahbub Alam & Muzahed Uddin Ahmed

  4. Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India

    Trailokya Nath Naik

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  1. Shyamal Kumar Paul
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Correspondence to Nobumichi Kobayashi.

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Paul, S.K., Kobayashi, N., Nagashima, S. et al. Phylogenetic analysis of rotaviruses with genotypes G1, G2, G9 and G12 in Bangladesh: evidence for a close relationship between rotaviruses from children and adults. Arch Virol 153, 1999–2012 (2008). https://doi.org/10.1007/s00705-008-0212-9

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