Skip to main content


Log in

A black-streaked dwarf disease on rice in China is caused by a novel fijivirus

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript


An isolate of a plant reovirus causing severe stunting and dark leaf symptoms on rice from Guangdong, China, was similar in virion morphology and serologically related to rice black-streaked dwarf virus (RBSDV). The electrophoretic profiles of genome segments of the two viruses in agarose or polyacrylamide gel were indistinguishable. The four genome segments of the new isolate corresponding to RBSDV S7–S10 were amplified by ligation RT-PCR and sequenced. The size and organization of each genome segment was very similar to its counterparts in RBSDV, maize rough dwarf virus (MRDV), and mal de Rio Cuarto virus (MRCV). Sequence identity was greatest to RBSDV and MRDV (ranging from about 60–85% depending on the protein), but identities were always much lower than those between RBSDV and MRDV. These comparisons and phylogenetic analyses suggested that the virus represents a new species in genus Fijivirus group 2, tentatively named Rice black-streaked dwarf virus-2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others


  1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  2. Anzola JV, Xu Z, Asamizu T, Nuss DL (1987) Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumour virus genome and defective interfering RNAs. Proc Natl Acad Sci USA 84:8301–8305

    Article  PubMed  CAS  Google Scholar 

  3. Boccardo G, Milne RG (1984) Plant reovirus group. CMI/AAB, Descriptions of Plant Viruses No. 294, Association of Applied Biologists, Wellesbourne, Warwick

  4. Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16:10881–10890

    Article  PubMed  CAS  Google Scholar 

  5. Distéfano AJ, Conci LR, Hidalgo MM, Guzmán FA, Hopp HE, del Vas M (2002) Sequence analysis of genome segments S4 and S8 of Mal de Río Cuarto virus (MRCV): evidence that the virus should be a separate Fijivirus species. Arch Virol 147:1699–1709

    Article  PubMed  Google Scholar 

  6. Distéfano AJ, Hopp HE, Del Vas M (2005) Sequence analysis of genome segments S5 and S10 of Mal de Rio Cuarto virus (Fijivirus, Reoviridae). Arch Virol 150:1241–1248

    Article  PubMed  Google Scholar 

  7. Distéfano AJ, Conci LR, Munoz Hidalgo M, Guzman FA, Hopp HE, del Vas M (2003) Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Rio Cuarto virus, a newly accepted Fijivirus species. Virus Res 92:113–121

    Article  PubMed  Google Scholar 

  8. Dong GK, Wang EG, Luo GL, Lin LW, Guan MP, Zhang ZD, Chen KS (1999) Occurrence of rice black-streaked dwarf disease in late-season hybrid rice and its control strategy. Acta Agric Zhejiang 11:364–367

    Google Scholar 

  9. Guzmán FA, Distéfano AJ, Arneodo JD, Hopp HE, Lenardon SL, del Vas M, Conci LR (2007) Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus. Arch Virol 152:565–573

    Article  PubMed  Google Scholar 

  10. Isogai M, Azuhata F, Uyeda I, Shikata E, Kimura I (1995) Genomic relationships between rice black-streaked dwarf and maize rough dwarf Fijiviruses detected by nucleic acid hybridization. Ann Phytopath Soc Japan 61:513–518

    CAS  Google Scholar 

  11. Isogai M, Uyeda I, Lee B-C (1998) Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9, and S10. J Gen Virol 79:1487–1494

    PubMed  CAS  Google Scholar 

  12. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  PubMed  CAS  Google Scholar 

  13. Li J, Li G, Yuan F (1999) Control of the maize rough dwarf virus disease. Beijing Agric Sci 17:23–25

    Google Scholar 

  14. Luisoni E, Lovisolo O, Kitagawa Y, Shikata E (1973) Serological relationship between maize rough dwarf virus and rice black streaked dwarf virus. Virology 52:281–283

    Article  PubMed  CAS  Google Scholar 

  15. Marzachi C, Boccardo G, Milne R, Isogai M, Uyeda I (1995) Genome structure and variability of fijiviruses. Sem Virol 6:103–108

    Article  CAS  Google Scholar 

  16. McMahon JA, Dale JL, Harding RM (1999) Taxonomic implications for Fijiviruses based on the terminal sequences of Fiji disease fijivirus. Arch Virol 144:2259–2263

    Article  PubMed  CAS  Google Scholar 

  17. McQualter RB, Burns P, Smith GR, Dale JL, Harding RM (2004) Molecular analysis of Fiji disease virus genome segments 5, 6, 8 and 10. Arch Virol 149:713–721

    Article  PubMed  CAS  Google Scholar 

  18. Milne RG, del Vas M, Harding RM, Marzachi R, Mertens PPC (2005) Genus Fijivirus. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. Eighth report of the international committee on taxonomy of viruses. Elsevier Academic Press, San Diego, pp 534–542

    Google Scholar 

  19. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  20. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  21. Uyeda I, Bong-Choon L, Ando Y (1998) Reovirus isolation and RNA extraction. In: Foster GD, Taylor SC (eds) Methods in molecular biology, vol 81. Plant virology protocols: from virus isolation to transgenic resistance. Humana Press, Totowa, pp 65–75

    Google Scholar 

  22. Van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens E, Estes MK, Lemon S, McGeoch D, Wickner RB, Mayo MA, Pringle CR, Maniloff J (eds) (2000) Virus taxonomy. Seventh report of the international committee on taxonomy of viruses. Academic Press, New York

    Google Scholar 

  23. Zhang HM, Chen JP, Adams MJ (2001) Molecular characterization of segments 1 to 6 of rice black-streaked dwarf virus from China provides the complete genome. Arch Virol 146:2331–2339

    Article  PubMed  CAS  Google Scholar 

  24. Zhang HM, Chen JP, Lei JL, Adams MJ (2001) Sequence analysis shows that a dwarfing disease on rice, maize and wheat in China is caused by Rice black-streaked dwarf virus (RBSDV). Eur J Plant Pathol 107:563–567

    Article  CAS  Google Scholar 

  25. Zhou GH, Xu DL, Li HP (2004) Identification of rice black-streaked dwarf virus infecting rice in Guangdong. In: Peng YL (ed) Proceedings of the conference on Chinese plant pathology. China Agricultural Scientech Press, Beijing, pp 210–212

    Google Scholar 

Download references


This work was funded by the China 863 program (2007AA10Z414) and 973 Program (2006CB708209), and the Zhejiang Provincial Foundation for Natural Science (Z305165). We thank Mr Xu Zhao-Wei, Station of Plant Protection of Yangxi County, Guangdong Province, for providing useful help during collection of virus-infected rice plants. Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom.

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Heng-Mu Zhang or Jian-Ping Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, HM., Yang, J., Chen, JP. et al. A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Arch Virol 153, 1893–1898 (2008).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: