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Use of a defective RNA of broad bean mottle bromovirus for stable gene expression in legumes

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Abstract

A viral vector that expressed foreign genes was engineered using a cDNA clone of broad bean mottle bromovirus (BBMV) defective interfering (DI) RNA. The expression vector required an active ORF to ensure efficient accumulation and replication in the host plants. After mechanical inoculation with BBMV RNAs, expression of the green fluorescent protein (GFP) reporter was driven by DI RNA constructs during consecutive passages through broad bean plants. Our data prove that BBMV DI RNAs, whose yield is similar to the virus genomic components, are useful as gene vectors after mechanical inoculation on legume plants.

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Acknowledgments

This study was supported by Ministerio de Educación y Ciencia, Spain (Grants AGL 01-0330 and RTA01-049). C. Sandoval completed this research as part of his PhD degree and was recipient of a fellowship from the Agencia Española de Cooperación Internacional (AECI). We are grateful to Francisco Sanchez and Brígida García for technical assistance.

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Author notes

  1. C. Sandoval

    Present address: Escuela de Agronomía, Universidad de Talca, Apartado postal 747, Talca, Chile

  2. J. Pogany

    Present address: Plant Pathology Department, University of Kentucky, Lexington, KY, 40546, USA

Authors and Affiliations

  1. Departamento de Protección Vegetal, INIA, Carretera de la Coruña km. 7.0, 28040, Madrid, Spain

    C. Sandoval & J. Romero

  2. Plant Molecular Biology Centre, Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA

    J. Pogany & J. Bujarski

  3. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland

    J. Bujarski

Authors
  1. C. Sandoval
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  2. J. Pogany
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  3. J. Bujarski
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  4. J. Romero
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Corresponding author

Correspondence to J. Romero.

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Sandoval, C., Pogany, J., Bujarski, J. et al. Use of a defective RNA of broad bean mottle bromovirus for stable gene expression in legumes. Arch Virol 153, 1755–1758 (2008). https://doi.org/10.1007/s00705-008-0174-y

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  • DOI: https://doi.org/10.1007/s00705-008-0174-y

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