Abstract
Multiple studies have established that GTPase activity is critical for MxA to act against RNA viruses. Recently, it was shown that MxA can also restrict the replication of hepatitis B virus (HBV), a DNA virus, but the requirements for GTPase activity in inhibition of HBV by MxA remain unknown. Here, we report that GTPase-defective mutants (K83A, T103A, and L612K) can downregulate extracellullar HBsAg and HBeAg and reduce the expression of extra- and intracellular HBV DNA in HepG2 cells to levels similar to that achieved by wild-type MxA. Furthermore, TMxA and T103, two nuclear forms of wild-type MxA and a GTPase-defective mutant (T103A) could only slightly decrease the expression of extra- and intracellular HBV DNA in HepG2 cells. In conclusion, GTPase activity is not essential for MxA protein to inhibit HBV replication, and MxA may have only a minimal effect on the replicative cycle of HBV in the nucleus.
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Acknowledgments
We thank Guylain Boulay, Sherbrooke Unniversity, Georg Kochs, Freiburg Unniversity, Masashi Mizokami, Nagoya City University, for kindly providing the related recombinant vectors. This work was supported by a grant from the Major State Basic Research (973) Program of the People’s Republic of China (No. 2007CB512900) and the Natural Science Foundation of China (30730082).
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Yu, Z., Wang, Z., Chen, J. et al. GTPase activity is not essential for the interferon-inducible MxA protein to inhibit the replication of hepatitis B virus. Arch Virol 153, 1677–1684 (2008). https://doi.org/10.1007/s00705-008-0168-9
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DOI: https://doi.org/10.1007/s00705-008-0168-9