Skip to main content

Advertisement

SpringerLink
Log in

Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

To analyze the role of the 2b protein (2bP) of Peanut stunt virus (PSV) in the viral infection cycle, we constructed PSV mutants that express either no 2bP or N-terminal-truncated 2bP. The accumulation of wild-type and mutant viruses in tobacco protoplasts indicated that the 2b cistron is not essential for viral replication. Viral accumulation in Nicotiana benthamiana plants suggested that the 2b cistron is responsible for viral accumulation in inoculated and upper leaves and has a role in virulence. The involvement of eight N-terminal amino acids of 2bP in these functions is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17:6739–6746

    Article  PubMed  CAS  Google Scholar 

  2. Ding SW, Anderson BJ, Haase HR, Symons RH (1994) New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601

    Article  PubMed  CAS  Google Scholar 

  3. Ding SW, Li WX, Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J 14:5762–5772

    PubMed  CAS  Google Scholar 

  4. Ding SW, Shi BJ, Li WX, Symons RH (1996) An interspecies hybrid RNA virus is significantly more virulent than either parental virus. Proc Natl Acad Sci USA 93:7470–7474

    Article  PubMed  CAS  Google Scholar 

  5. Hayes RJ, Buck KW (1990) Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368

    Article  PubMed  CAS  Google Scholar 

  6. Hu CC, Aboul-Ata AE, Naidu RA, Ghabrial SA (1997) Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA 3. J Gen Virol 78:929–939

    PubMed  CAS  Google Scholar 

  7. Hu CC, Sanger M, Ghabrial SA (1998) Production of infectious RNA transcripts from full-length cDNA clones representing two subgroups of peanut stunt virus strains: mapping satellite RNA support to RNA 1. J Gen Virol 79:2013–2021

    PubMed  CAS  Google Scholar 

  8. Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1991) Nucleotide sequence of RNA 3 of peanut stunt cucumovirus. Virology 185:464–467

    Article  PubMed  CAS  Google Scholar 

  9. Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1992) Nucleotide sequence analysis of peanut stunt cucumovirus RNAs 1 and 2. J Gen Virol 73:701–707

    Article  PubMed  CAS  Google Scholar 

  10. Lu R, Folimonov A, Shintaku M, Li WX, Falk BW, Dawson WO, Ding SW (2004) Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci USA 101:15742–15747

    Article  PubMed  CAS  Google Scholar 

  11. Lucy AP, Guo HS, Li WX, Ding SW (2000) Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. EMBO J 19:1672–1680

    Article  PubMed  CAS  Google Scholar 

  12. Nitta N, Takanami Y, Kuwata S, Kubo S (1988) Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J Gen Virol 69:2695–2700

    Article  CAS  Google Scholar 

  13. Palukaitis P, García-Arenal F (2003) Cucumoviruses. Adv Virus Res 62:241–323

    Article  PubMed  CAS  Google Scholar 

  14. Shi BJ, Ding SW, Symons RH (1997) In vivo expression of an overlapping gene encoded by the cucumoviruses. J Gen Virol 78:237–241

    PubMed  CAS  Google Scholar 

  15. Sivakumaran K, Chen MH, Roossinck MJ, Kao CC (2002) Core promoter for initiation of Cucumber mosaic virus subgenomic RNA 4A. Mol Plant Pathol 3:43–52

    Article  CAS  Google Scholar 

  16. Soards AJ, Murphy AM, Palukaitis P, Carr JP (2002) Virulence and differential local and systemic spread of Cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Mol Plant Microbe Interact 15:647–653

    Article  PubMed  CAS  Google Scholar 

  17. Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y (1991) Functional analysis of deletion mutants of cucumber mosaic virus RNA 3 using an in vitro transcription system. Virology 183:106–113

    Article  PubMed  CAS  Google Scholar 

  18. Suzuki M, Kuwata S, Masuta C, Takanami Y (1995) Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco. J Gen Virol 76:1791–1799

    Article  PubMed  CAS  Google Scholar 

  19. Suzuki M, Yoshida M, Yoshinuma T, Hibi T (2003) Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus. J Gen Virol 84:1931–1939

    Article  PubMed  CAS  Google Scholar 

  20. Takeshita M, Suzuki M, Kuwata S, Takanami Y (1998) Involvement of cucumber mosaic virus RNA 2 and RNA 3 in viral systemic spread in radish plant. Arch Virol 143:1109–1117

    Article  PubMed  CAS  Google Scholar 

  21. Takeshita M, Suzuki M, Takanami Y (2001) Combination of amino acids in the 3a protein and the coat protein of Cucumber mosaic virus determines symptom expression and viral spread in bottle gourd. Arch Virol 146:697–711

    Article  PubMed  CAS  Google Scholar 

  22. Tsuchizaki T (1973) Peanut stunt virus isolated from beans in Japan. Ann Phytopath Soc Jpn 39:67–72

    Google Scholar 

  23. Yan LY, Xu ZY, Goldbach R, Kunrong C, Prins M (2005) Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China. Arch Virol 150:1203–1211

    Article  PubMed  CAS  Google Scholar 

  24. Zhang X, Yuan YR, Pei Y, Lin SS, Tuschl T, Patel DJ, Chua NH (2006) Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes Dev 20:3255–3268

    Article  PubMed  CAS  Google Scholar 

  25. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucl Acids Res 31:3406–3415

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (no. 18380029).

Author information

Authors and Affiliations

  1. Laboratory of Bioresource Technology, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 202 Bioscience Building, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan

    O. Netsu, M. Ugaki & M. Suzuki

  2. Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama, 240-8501, Japan

    K. Hiratsuka

  3. Laboratory of Plant Biotechnology, Department of Life Sciences, School of Agriculture, Meiji University, Tama-ku, Kawasaki, 214-8571, Japan

    S. Kuwata

  4. Plant Clinical Science Course, Faculty of Bioscience and Applied Chemistry, Hosei University, 3-7-2 Kajino-cho, Koganei, Tokyo, 184-8584, Japan

    T. Hibi

Authors
  1. O. Netsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Hiratsuka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Kuwata
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Hibi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Ugaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Suzuki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Netsu, O., Hiratsuka, K., Kuwata, S. et al. Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana . Arch Virol 153, 1731–1735 (2008). https://doi.org/10.1007/s00705-008-0166-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-008-0166-y

Keywords

Search

Navigation