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Identification of viral and non-viral reverse transcribing elements in pineapple (Ananas comosus), including members of two new badnavirus species

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Abstract

A previously published partial sequence of pineapple bacilliform virus was shown to be from a retrotransposon (family Metaviridae) and not from a badnavirus as previously thought. Two newly discovered sequence groups isolated from pineapple were associated with bacilliform virions and were transmitted by mealybugs. Phylogenetic analyses indicated that they were members of new badnavirus species. A third caulimovirid sequence was also amplified from pineapple, but available evidence suggests that this DNA is not encapsidated, but more likely derived from an endogenous virus.

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References

  1. Carter W (1963) Mealybug wilt of pineapple; a reappraisal. Ann NY Acad Sci 105:741–764

    Article  Google Scholar 

  2. Gambley CF, Steele V, Geering ADW, Thomas JE (2008) The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease. Australas Plant Pathol 37:95–105

    Article  CAS  Google Scholar 

  3. Gawel NJ, Jarret RL (1991) A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Mol Biol Rep 9(3):262–266

    Article  CAS  Google Scholar 

  4. Geering ADW, Olszewski NE, Harper G, Lockhart BEL, Hull R, Thomas JE (2005) Banana contains a diverse array of endogenous badnaviruses. J Gen Virol 86:511–520

    Article  PubMed  CAS  Google Scholar 

  5. Geijskes RJ, Braithwaite KS, Dale JL, Harding RM, Smith GR (2002) Sequence analysis of an Australian isolate of sugarcane bacilliform badnavirus. Arch Virol 147:2393–2404

    Article  PubMed  CAS  Google Scholar 

  6. German TL, Ullman DE, Gunashinghe UB (1992) Mealybug wilt of pineapple. In: Harris KF (ed) Advances in Disease Vector Research. Springer-Verlag, New York, pp 241–259

    Google Scholar 

  7. Harper G, Hart D, Moult S, Hull R, Geering A, Thomas J (2005) The diversity of Banana streak virus isolates in Uganda. Arch Virol 150:2407–2420

    Article  PubMed  CAS  Google Scholar 

  8. Hull R, Covey SN (1996) Retroelements: Propagation and adaptation. Virus Genes 11(2):105–118

    Article  Google Scholar 

  9. Hull R, Geering ADW, Harper G, Lockhart BE, Schoelz JE (2005) Virus Taxonomy. Family Caulimoviridae. Eighth Report of the International Committee on Taxonomy of Viruses. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Elsevier Academic Press, San Diego, pp 385–396

  10. Ito K (1959) Terminal mottle as a symptomatological aspect of mealybug wilt with evidence supporting the hypothesis of a virus etiology of the disease. Pineapple Research Institute, 62, Hawaii

  11. Kumar A, Bennetzen JL (1999) Plant retrotransposons. Annu Rev Genet 33(1):479–532

    Article  PubMed  CAS  Google Scholar 

  12. Lockhart BEL, Olszewski NE (1993) Serological and Genomic Heterogeneity of Banana Streak Badnavirus: Implications for Virus Detection in Musa Germplasm. In: Ganry J (ed) Breeding bananas and plantain for resistance to disease and pests. Montpellier, France, pp 105–113, CIRAD in collaboration with INIBAP

  13. Muller E, Sackey S (2005) Molecular variability analysis of five new complete Cacao swollen shoot virus genomic sequences. Arch Virol 150:53–66

    Article  PubMed  CAS  Google Scholar 

  14. Qu R, Bhattacharyya M, Laco GS, de Kochko A, Rao BLS, Kaniewska MB, Elmer JS, Rochester DE, Smith CE, Beachy RN (1991) Characterization of the rice tungro bacilliform virus: comparison with Commelina yellow mottle virus and caulimoviruses. Virology 185:354–364

    Article  PubMed  CAS  Google Scholar 

  15. Sether DM, Hu JS (2002) Closterovirus infection and mealybug exposure are necessary for the development of mealybug wilt of pineapple disease. Phytopathology 92:928–935

    Article  Google Scholar 

  16. Sørensen AB, Duch M, Jørgensen P, Pedersen FS (1993) Amplification and sequence analysis of DNA flanking integrated provirus by a simple two-step polymerase chain reaction method. J Virol 67(12):7118–7124

    PubMed  Google Scholar 

  17. Staginnus C, Richert-Poggeler KR (2006) Endogenous pararetroviruses: two-faced travellers in the plant genome. Trends Plant Sci 11:485–491

    Article  PubMed  CAS  Google Scholar 

  18. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  19. Thomson KG, Dietzgen RG, Thomas JE, Teakle DS (1996) Detection of pineapple bacilliform virus using the polymerase chain reaction. Ann Appl Biol 129:57–69

    Article  CAS  Google Scholar 

  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  21. Wakman W, Teakle DS, Thomas JE, Dietzgen RG (1995) Presence of a clostero-like virus and a bacilliform virus in pineapple plants in Australia. Aust J Agr Res 46:947–958

    Article  Google Scholar 

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Acknowledgments

We thank Horticulture Australia Limited, DPI&F, Golden Circle and the University of Queensland (UQ) for funding and Dr. E. Aitken (UQ) for helpful discussions, Dr. G. Sanewski (DPI&F) and Mr. J.-P. Horry (CIRAD) for supplying germplasm accessions.

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Authors and Affiliations

  1. Department of Primary Industries and Fisheries, Horticulture and Forestry Science, 80 Meiers Road, Indooroopilly, Queensland, 4068, Australia

    C. F. Gambley, A. D. W. Geering, V. Steele & J. E. Thomas

  2. School of Integrative Biology, The University of Queensland, St Lucia, Queensland, 4072, Australia

    C. F. Gambley

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  1. C. F. Gambley
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  2. A. D. W. Geering
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  3. V. Steele
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  4. J. E. Thomas
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Correspondence to C. F. Gambley.

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Gambley, C.F., Geering, A.D.W., Steele, V. et al. Identification of viral and non-viral reverse transcribing elements in pineapple (Ananas comosus), including members of two new badnavirus species. Arch Virol 153, 1599–1604 (2008). https://doi.org/10.1007/s00705-008-0160-4

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  • DOI: https://doi.org/10.1007/s00705-008-0160-4

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