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Identification of a single-nucleocapsid baculovirus isolated from Clanis bilineata tsingtauica (Lepidoptera: Sphingidae)

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Abstract

A nucleopolyhedrovirus isolated from infected larvae of Clanis bilineata tsingtauica was characterized. Electron microscopical studies on the ultrastructure of C. bilineata nucleopolyhedrovirus (ClbiSNPV) occlusion bodies (OBs) showed several virions (up to 16) with a single nucleocapsid packaged within a single viral envelope. The diameter of the OBs was 0.77–1.7 μm with a mean of 1.13 ± 0.19 μm. The complete sequence of the ClbiSNPV polyhedrin (polh) gene contained 741 nucleotides, predicting a protein of 246 amino acids. Phylogenetic analyses using the complete sequence of the polh genes indicated that ClbiSNPV clusters with Group II NPVs. This is the first record of a baculovirus from C. bilineata.

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Acknowledgments

We thank Prof. Chuanxi Zhang (Institute of Insect Science, Zhejiang University) for his helpful suggestions. This work was supported by the 973 National Basic Research Program of China (2005CB121005), the Six-Field Top programs of Jiangsu Provice, the National Natural Science Foundation of Jiangsu Education Community (06KJD180043), and the Innovation Foundation for Graduate Students of Jiangsu Province.

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Authors and Affiliations

  1. Institute of Life Sciences, Jiangsu University, Xuefu Road 301, 212013, Zhenjiang, China

    Liqun Wang, Shanying Zhu, Yan Chen & Wenbing Wang

  2. Department of Chemical Engineering, Jiangsu Ploytechnic University, Changzhou, China

    Liqun Wang

  3. Shanghai Entry-Exit Quarantine and Inspection, Bureau, China

    Jianping Yi

  4. School of Life Sciences, Soochow University, Suzhou, China

    Bing Li & Weide Shen

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  1. Liqun Wang
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  2. Jianping Yi
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  3. Shanying Zhu
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  4. Bing Li
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  5. Yan Chen
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  6. Weide Shen
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  7. Wenbing Wang
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Correspondence to Wenbing Wang.

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Wang, L., Yi, J., Zhu, S. et al. Identification of a single-nucleocapsid baculovirus isolated from Clanis bilineata tsingtauica (Lepidoptera: Sphingidae). Arch Virol 153, 1557–1561 (2008). https://doi.org/10.1007/s00705-008-0153-3

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  • DOI: https://doi.org/10.1007/s00705-008-0153-3

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