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A mutational shift from domain III to II in the internal ribosome entry site of hepatitis C virus after interferon–ribavirin therapy

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Abstract

We focused on the relationship between variation in the IRES of hepatitis C virus (HCV) genotype 1b and clinical outcome, since the internal ribosome entry site (IRES) has a comparatively low heterogeneity and it might be easy to find unique substitutions. Patients infected with HCV were selected using strict criteria, and unique mutations in the IRES were extracted by the subtraction of common mutations. We found that most mutations accumulated in domain III (dIII) of IRES in sustained virological responders (SVRs) and non-SVRs before therapy. However, these mutations were exclusively observed in domain II (dII) in non-SVR at 2 weeks after the start of therapy.

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Acknowledgments

We would like to thank to T. Sayano (Kyushu University), K. Yasuda (Department of Parasitology, Kurume University School of Medicine) and Y. Imamura (Division of Infectious Diseases, Department of Infectious Medicine, Kurume University School of Medicine) for their valuable technical assistance. We also thank T. Omura for his critical reading of the manuscript.

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Authors and Affiliations

  1. Division of Infectious Diseases, Department of Infectious Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan

    Kei Ogata, Takahito Kashiwagi, Jun Iwahashi, Koyu Hara, Haruhito Honda, Hiroshi Watanabe & Nobuyuki Hamada

  2. Division of Gastroenterology, Department of Internal Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan

    Kei Ogata, Tatsuya Ide, Ryukichi Kumashiro & Michio Sata

  3. Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Biology, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, 113-8613, Japan

    Michinori Kohara

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  1. Kei Ogata
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  2. Takahito Kashiwagi
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  3. Jun Iwahashi
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  4. Koyu Hara
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  5. Haruhito Honda
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  6. Tatsuya Ide
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  7. Ryukichi Kumashiro
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  8. Michinori Kohara
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  9. Michio Sata
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  10. Hiroshi Watanabe
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  11. Nobuyuki Hamada
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Correspondence to Nobuyuki Hamada.

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Ogata, K., Kashiwagi, T., Iwahashi, J. et al. A mutational shift from domain III to II in the internal ribosome entry site of hepatitis C virus after interferon–ribavirin therapy. Arch Virol 153, 1575–1579 (2008). https://doi.org/10.1007/s00705-008-0143-5

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  • DOI: https://doi.org/10.1007/s00705-008-0143-5

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