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Archives of Virology

, Volume 153, Issue 5, pp 891–897 | Cite as

Serologic and hexon phylogenetic analysis of ruminant adenoviruses

  • Howard D. LehmkuhlEmail author
  • L. A. Hobbs
Original Article

Abstract

The objectives of this study were to determine the antigenic relationship among ruminant adenoviruses and determine their phylogenetic relationship based on the deduced hexon gene amino acid sequence. Results of reciprocal cross-neutralization tests demonstrated antigenic relationships in either one or both directions among bovine adenovirus type 6 (BAdV-6), BAdV-7, ovine adenovirus type 7 (OAdV-7), caprine adenovirus type 1 (GAdV-1), and deer adenovirus (Odocoileus adenovirus 1, OdAdV-1). No antigenic cross-reactivity was observed among BAdV-1 through -5, and -8 and the other putative ruminant adenoviruses. Two PCR primer sets, one for mastadenovirus and atadenovirus that amplified an approximately 2,700-bp region in the hexon genes were used for comparative studies. Phylogenetic analysis of the deduced hexon amino acid sequences clustered the ruminant adenoviruses on the Mastadenovirus and Atadenovirus genus branches of the Adenoviridae tree. The recent classification of BAdV-6, and -7 as members of the genus Atadenovirus was supported by phylogenetic distance matrix analysis of their deduced hexon amino acid sequences. Further, we propose that BAdV-6 and -7 be recognized as members of new Atadenovirus species, Bovine adenovirus E and Bovine adenovirus F, respectively. Phylogenetic analysis of OdAdV-1 places this virus in the genus Atadenovirus with a proposed new species Odocoileus adenovirus A. OAdV-6 and GAdV-2 are proposed as members of new Mastadenovirus species Ovine adenovirus C and Goat adenovirus A, respectively.

Keywords

Adenovirus Type Hexon Antigenic Relationship Hexon Gene Titer Ratio 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

I am grateful to Dr. Brian M. Adair for providing adenoviruses used in this study.

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Copyright information

© U.S. Government 2008

Authors and Affiliations

  1. 1.Respiratory Disease of Livestock Research Unit, National Animal Disease Center, Agricultural Research ServiceUnited States Department of AgricultureAmesUSA

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