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Phylogeny and molecular genetic parameters of different stages of hepatitis B virus infection in patients from the Brazilian Amazon

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Abstract

A fragment of 600 bp of the gene which codes for the surface antigen of hepatitis B virus (HBV) was amplified and sequenced from patients who were born in five states of the Brazilian Amazon (Amazonas, Pará, Acre, Rondônia and Tocantins). A total of 44 sequences were used for the estimation of molecular genetic parameters and phylogenetic analyses. Compared with patients who were asymptomatic, those who had acute hepatitis and chronic liver disease had higher levels of genetic variability and higher rates of nucleotide substitutions. The analysis of transition and transversion substitutions showed that transition-type substitutions predominated. In chronic liver disease carriers, transversion-type substitutions showed phylogenetic saturation. In general, all of the analyses carried out in this study showed an association between patterns of changes in molecular genetic parameters and the stage of disease progression. Phylogenetic analysis using the HKY85 model of evolution identified 41 individuals as genotype A, suggesting its predominance in the Amazon region, one individual as genotype C, and one individual closely related to genotypes E and F.

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Acknowledgments

This work was supported by Universidade Federal do Amazonas. The authors are grateful to Fundação de Medicina Tropical do Amazonas for technical assistance, samples and collaborations. Permission to collect blood samples have been granted by Comitê de Ética em Pesquisa da Fundação de Medicina Tropical do Amazonas (FMTAM) (License No. 158/99). We thank Tomas Hrbek and two anonymous referees for comments and suggestions on the manuscript. This work forms a portion of C.M.C.O. Ph D thesis at the Biotechnology program of Universidade Federal do Amazonas.

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Authors and Affiliations

  1. Centro de Apoio Multidisciplinar, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, AM, Brazil

    Cíntia Mara de Oliveira & Spartaco Astolfi-Filho

  2. Laboratório de Evolução e Genética Animal, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, AM, Brazil

    Izeni Pires Farias

  3. Gerência de Virologia, Fundação de Medicina Tropical, Manaus, AM, Brazil

    José Carlos Ferraz da Fonseca, Leila Melo Brasil & Rita de Souza

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  1. Cíntia Mara de Oliveira
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  2. Izeni Pires Farias
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Correspondence to Izeni Pires Farias.

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de Oliveira, C.M., Farias, I.P., da Fonseca, J.C.F. et al. Phylogeny and molecular genetic parameters of different stages of hepatitis B virus infection in patients from the Brazilian Amazon. Arch Virol 153, 823–830 (2008). https://doi.org/10.1007/s00705-008-0053-6

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  • DOI: https://doi.org/10.1007/s00705-008-0053-6

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