Skip to main content

Advertisement

SpringerLink
Log in

Functional rescue of an oligomerization-defective HIV-1 Rev mutant by fusion with an oligomeric tag

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The HIV-1 wild-type Rev and the negative oligomerization-defective mutant RevM4 were fused to enhanced green fluorescent protein (EGFP) and the tetrameric red fluorescent protein (DsRed1) followed by examination of their intracellular localization and Rev activity. As previously shown, fusion of EGFP to Rev and RevM4 did not affect the biological activity. Fusion of DsRed1 to Rev inhibited activity, and interestingly, fusion of DsRed1 to RevM4 restored activity. Based on these results, a model is proposed suggesting how RevM4-DsRed1 is able to rescue Rev activity through oligomerization on the viral RNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • AW Cochrane A Perkins CA Rosen (1990) ArticleTitleIdentification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: relevance of nucleolar localization to function J Virol 64 881–885 Occurrence Handle2404140 Occurrence Handle1:CAS:528:DyaK3cXpsFamtA%3D%3D

    PubMed  CAS  Google Scholar 

  • BP Cormack RH Valdivia S Falkow (1996) ArticleTitleFACS-optimized mutants of the green fluorescent protein (GFP) Gene 173 33–38 Occurrence Handle8707053 Occurrence Handle10.1016/0378-1119(95)00685-0 Occurrence Handle1:CAS:528:DyaK28XktVOlu7c%3D

    Article  PubMed  CAS  Google Scholar 

  • AI Dayton EF Terwilliger J Potz M Kowalski JG Sodroski WA Haseltine (1988) ArticleTitleCis-acting sequences responsive to the rev gene product of the human immunodeficiency virus J Acquir Immune Defic Syndr 1 441–452 Occurrence Handle2851651 Occurrence Handle1:STN:280:BiaC38rjsFQ%3D

    PubMed  CAS  Google Scholar 

  • U Fischer J Huber WC Boelens IW Mattaj R Luhrmann (1995) ArticleTitleThe HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs Cell 82 475–483 Occurrence Handle7543368 Occurrence Handle10.1016/0092-8674(95)90436-0 Occurrence Handle1:CAS:528:DyaK2MXnsFSgtrs%3D

    Article  PubMed  CAS  Google Scholar 

  • M Fornerod M Ohno M Yoshida IW Mattaj (1997) ArticleTitleCRM1 is an export receptor for leucine-rich nuclear export signals Cell 90 1051–1060 Occurrence Handle9323133 Occurrence Handle10.1016/S0092-8674(00)80371-2 Occurrence Handle1:CAS:528:DyaK2sXmtlOntbk%3D

    Article  PubMed  CAS  Google Scholar 

  • M Fukuda S Asano T Nakamura M Adachi M Yoshida M Yanagida E Nishida (1997) ArticleTitleCRM1 is responsible for intracellular transport mediated by the nuclear export signal Nature 390 308–311 Occurrence Handle9384386 Occurrence Handle10.1038/36894 Occurrence Handle1:CAS:528:DyaK2sXnsVOrs70%3D

    Article  PubMed  CAS  Google Scholar 

  • C Furnes T Arnesen P Askjaer J Kjems AM Szilvay (2005) ArticleTitleHIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain Retrovirology 2 39 Occurrence Handle15949040 Occurrence Handle10.1186/1742-4690-2-39

    Article  PubMed  Google Scholar 

  • HH Gerdes C Kaether (1996) ArticleTitleGreen fluorescent protein: applications in cell biology FEBS Lett 389 44–47 Occurrence Handle8682203 Occurrence Handle10.1016/0014-5793(96)00586-8 Occurrence Handle1:CAS:528:DyaK28XjvFGhsrk%3D

    Article  PubMed  CAS  Google Scholar 

  • LA Gross GS Baird RC Hoffman KK Baldridge RY Tsien (2000) ArticleTitleThe structure of the chromophore within DsRed, a red fluorescent protein from coral Proc Natl Acad Sci USA 97 11990–11995 Occurrence Handle11050230 Occurrence Handle10.1073/pnas.97.22.11990 Occurrence Handle1:CAS:528:DC%2BD3cXnvVSgtrs%3D

    Article  PubMed  CAS  Google Scholar 

  • TJ Hope D McDonald XJ Huang J Low TG Parslow (1990) ArticleTitleMutational analysis of the human immunodeficiency virus type 1 Rev transactivator: essential residues near the amino terminus J Virol 64 5360–5366 Occurrence Handle2120472 Occurrence Handle1:CAS:528:DyaK3cXmt1KltbY%3D

    PubMed  CAS  Google Scholar 

  • KH Kalland AM Szilvay KA Brokstad W Saetrevik G Haukenes (1994) ArticleTitleThe human immunodeficiency virus type 1 Rev protein shuttles between the cytoplasm and nuclear compartments Mol Cell Biol 14 7436–7444 Occurrence Handle7935458 Occurrence Handle1:CAS:528:DyaK2cXmslKnurg%3D

    PubMed  CAS  Google Scholar 

  • KH Kalland AM Szilvay E Langhoff G Haukenes (1994) ArticleTitleSubcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm J Virol 68 1475–1485 Occurrence Handle8107211 Occurrence Handle1:CAS:528:DyaK2cXhvV2mu7k%3D

    PubMed  CAS  Google Scholar 

  • J Kjems M Brown DD Chang PA Sharp (1991) ArticleTitleStructural analysis of the interaction between the human immunodeficiency virus Rev protein and the Rev response element Proc Natl Acad Sci USA 88 683–687 Occurrence Handle1992459 Occurrence Handle10.1073/pnas.88.3.683 Occurrence Handle1:CAS:528:DyaK3MXhsl2itr8%3D

    Article  PubMed  CAS  Google Scholar 

  • S Kubota RJ Pomerantz (1998) ArticleTitleA cis-acting peptide signal in human immunodeficiency virus type I Rev which inhibits nuclear entry of small proteins Oncogene 16 1851–1861 Occurrence Handle9583682 Occurrence Handle10.1038/sj.onc.1201738 Occurrence Handle1:CAS:528:DyaK1cXivVWku70%3D

    Article  PubMed  CAS  Google Scholar 

  • MH Malim S Bohnlein J Hauber BR Cullen (1989) ArticleTitleFunctional dissection of the HIV-1 Rev trans-activator-derivation of a trans-dominant repressor of Rev function Cell 58 205–214 Occurrence Handle2752419 Occurrence Handle10.1016/0092-8674(89)90416-9 Occurrence Handle1:CAS:528:DyaL1MXlt12htL8%3D

    Article  PubMed  CAS  Google Scholar 

  • MH Malim DF McCarn LS Tiley BR Cullen (1991) ArticleTitleMutational definition of the human immunodeficiency virus type 1 Rev activation domain J Virol 65 4248–4254 Occurrence Handle2072452 Occurrence Handle1:CAS:528:DyaK3MXltFSiu7g%3D

    PubMed  CAS  Google Scholar 

  • MV Matz AF Fradkov YA Labas AP Savitsky AG Zaraisky ML Markelov SA Lukyanov (1999) ArticleTitleFluorescent proteins from nonbioluminescent Anthozoa species Nat Biotechnol 17 969–973 Occurrence Handle10504696 Occurrence Handle10.1038/13657 Occurrence Handle1:CAS:528:DyaK1MXmvVelsrk%3D

    Article  PubMed  CAS  Google Scholar 

  • BE Meyer MH Malim (1994) ArticleTitleThe HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm Genes Dev 8 1538–1547 Occurrence Handle7958838 Occurrence Handle10.1101/gad.8.13.1538 Occurrence Handle1:CAS:528:DyaK2cXlt12msbk%3D

    Article  PubMed  CAS  Google Scholar 

  • M Neville F Stutz L Lee LI Davis M Rosbash (1997) ArticleTitleThe importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export Curr Biol 7 767–775 Occurrence Handle9368759 Occurrence Handle10.1016/S0960-9822(06)00335-6 Occurrence Handle1:CAS:528:DyaK2sXntVKltbg%3D

    Article  PubMed  CAS  Google Scholar 

  • T Nosaka T Takamatsu Y Miyazaki K Sano A Sato S Kubota M Sakurai Y Ariumi M Nakai S Fujita et al. (1993) ArticleTitleCytotoxic activity of rev protein of human immunodeficiency virus type 1 by nucleolar dysfunction Exp Cell Res 209 89–102 Occurrence Handle8224012 Occurrence Handle10.1006/excr.1993.1289 Occurrence Handle1:CAS:528:DyaK2cXlt1Chug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • R Stauber GA Gaitanaris GN Pavlakis (1995) ArticleTitleAnalysis of trafficking of Rev and transdominant Rev proteins in living cells using green fluorescent protein fusions: transdominant Rev blocks the export of Rev from the nucleus to the cytoplasm Virology 213 439–449 Occurrence Handle7491768 Occurrence Handle10.1006/viro.1995.0016 Occurrence Handle1:CAS:528:DyaK2MXpsVGiu7k%3D

    Article  PubMed  CAS  Google Scholar 

  • A Szebeni JE Herrera MO Olson (1995) ArticleTitleInteraction of nucleolar protein B23 with peptides related to nuclear localization signals Biochemistry 34 8037–8042 Occurrence Handle7794916 Occurrence Handle10.1021/bi00025a009 Occurrence Handle1:CAS:528:DyaK2MXmtVyitL4%3D

    Article  PubMed  CAS  Google Scholar 

  • AM Szilvay KA Brokstad R Kopperud G Haukenes KH Kalland (1995) ArticleTitleNuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants J Virol 69 3315–3323 Occurrence Handle7745679 Occurrence Handle1:CAS:528:DyaK2MXls1yisbY%3D

    PubMed  CAS  Google Scholar 

  • AM Szilvay KA Brokstad SO Boe G Haukenes KH Kalland (1997) ArticleTitleOligomerization of HIV-1 Rev mutants in the cytoplasm and during nuclear import Virology 235 73–81 Occurrence Handle9300038 Occurrence Handle10.1006/viro.1997.8671 Occurrence Handle1:CAS:528:DyaK2sXlsFGjt78%3D

    Article  PubMed  CAS  Google Scholar 

  • AM Szilvay SO Boe KH Kalland (1999) ArticleTitleCo-expression of a trans-dominant negative mutant of the human immunodeficiency virus type 1 (HIV-1) Rev protein affects the Rev-dependent splicing pattern and expression of HIV-1 RNAs J Gen Virol 80 IssueIDPt 8 1965–1974 Occurrence Handle10466792 Occurrence Handle1:CAS:528:DyaK1MXlt1aqtbs%3D

    PubMed  CAS  Google Scholar 

  • TO Tange TH Jensen J Kjems (1996) ArticleTitleIn vitro interaction between human immunodeficiency virus type 1 Rev protein and splicing factor ASF/SF2-associated protein, p32 J Biol Chem 271 10066–10072 Occurrence Handle8626563 Occurrence Handle10.1074/jbc.271.17.10066 Occurrence Handle1:CAS:528:DyaK28XisFGqurc%3D

    Article  PubMed  CAS  Google Scholar 

  • B Wolff G Cohen J Hauber D Meshcheryakova C Rabeck (1995) ArticleTitleNucleocytoplasmic transport of the Rev protein of human immunodeficiency virus type 1 is dependent on the activation domain of the protein Exp Cell Res 217 31–41 Occurrence Handle7867718 Occurrence Handle10.1006/excr.1995.1060 Occurrence Handle1:CAS:528:DyaK2MXktVGnt7w%3D

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, University of Bergen, Bergen, Norway

    C. Furnes, V. Andresen & A. M. Szilvay

Authors
  1. C. Furnes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Andresen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Szilvay
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Furnes.

Additional information

Present address: C. Furnes, Norwegian College of Fishery Science, University of Tromsø, N-9037 Tromsø, Norway

Present address: V. Andresen, Animals Models and Retroviral Vaccines Section, National Cancer Institute, Bethesda, 20892-5065 Maryland, U.S.A.

Correspondence: Clemens Furnes, Department of Molecular Biology, University of Bergen, HIB, Box 7803, N-5020 Bergen, Norway

Rights and permissions

Reprints and permissions

About this article

Cite this article

Furnes, C., Andresen, V. & Szilvay, A. Functional rescue of an oligomerization-defective HIV-1 Rev mutant by fusion with an oligomeric tag. Arch Virol 153, 357–362 (2008). https://doi.org/10.1007/s00705-007-1095-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-007-1095-x

Keywords

Search

Navigation