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Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomal-lysosomal system acidification

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Summary

Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-γ) or endosomal- lysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 ± 39% increase), IFN-γ (446 ± 8%), ammonium chloride + IFN-γ (1721 ± 283%), chloroquine diphosphate (1037 ± 121%), chloroquine diphosphate + IFN-γ (2199 ± 255%), monensin (950 ± 178%) and monensin + IFN-γ (1948 ± 60%). Combined IFN-γ and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.

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Authors and Affiliations

  1. Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

    G. Misinzo, P. L. Delputte, D. J. Lefebvre & H. J. Nauwynck

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  1. G. Misinzo
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  2. P. L. Delputte
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  3. D. J. Lefebvre
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  4. H. J. Nauwynck
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Correspondence to H. J. Nauwynck.

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Correspondence: Hans J. Nauwynck, Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium

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Misinzo, G., Delputte, P., Lefebvre, D. et al. Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomal-lysosomal system acidification. Arch Virol 153, 337–342 (2008). https://doi.org/10.1007/s00705-007-1092-0

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