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Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants

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Summary

Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses.

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Authors and Affiliations

  1. The S. Tolkowsky Laboratory, Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel

    Y. Moskovitz, L. Bir, A. Fingstein & M. Mawassi

  2. The Virology Unit, Agricultural Research Council, Plant Protection Research Institute, Pretoria, South Africa

    D. E. Goszczynski

  3. The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel

    H. Czosnek

Authors
  1. Y. Moskovitz
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  2. D. E. Goszczynski
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  3. L. Bir
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  4. A. Fingstein
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  5. H. Czosnek
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  6. M. Mawassi
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Corresponding author

Correspondence to M. Mawassi.

Additional information

Correspondence: Munir Mawassi, The S. Tolkowsky Laboratory, Department of Virology, Agricultural Research Organization, the Volcani Center, Bet Dagan 50250, Israel

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Moskovitz, Y., Goszczynski, D., Bir, L. et al. Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants. Arch Virol 153, 323–328 (2008). https://doi.org/10.1007/s00705-007-1075-1

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  • DOI: https://doi.org/10.1007/s00705-007-1075-1

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