Skip to main content
SpringerLink
Log in

Varying genetic diversity of Papaya ringspot virus isolates from two time-separated outbreaks in Jamaica and Venezuela

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Coat protein sequences of 22 Papaya ringspot virus isolates collected from different locations in Jamaica and Venezuela in 1999 and 2004, respectively, were determined and compared with sequences of isolates from earlier epidemics in 1990 and 1993. Jamaican isolates collected in 1999 exhibited nucleotide sequence identities between 98 and 100% but shared lower identities of 92.2% with an isolate collected in 1990. Isolates from the 2004 epidemic in Venezuela exhibited more heterogeneity, with identities between 88.7 and 98.8%. However, isolates collected in 1993 were more closely related (97.7%). The viral populations of the two countries are genetically different and appear to be changing at different rates; presumably driven by introductions, movement of plant materials, geographical isolation, and disease management practices.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • M Bateson R Lines R Revill W Chaleeprom C Ha A Gibbs J Dale (2002) ArticleTitleOn the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus J Gen Virol 83 2575–2585 Occurrence Handle12237441 Occurrence Handle1:CAS:528:DC%2BD38XnsFKnsrk%3D

    PubMed  CAS  Google Scholar 

  • M Bateson J Henderson W Chaleeprom A Gibbs J Dale (1994) ArticleTitlePapaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia) J Gen Virol 75 3547–3553 Occurrence Handle7996146 Occurrence Handle1:CAS:528:DyaK2MXisFKgs7s%3D

    PubMed  CAS  Google Scholar 

  • MJ Davis Z Ying (1999) ArticleTitleGenetic diversity of the papaya ringspot virus in Florida Proc Fla State Hort Soc 112 194–196

    Google Scholar 

  • Fermin G (1996) Análisis molecular de variantes geográficos del virus de la mancha anular de la lechosa (PRV) y cloneo de diversos constructos. MS thesis, Universad de Los Andes, Mérida

  • G Fermin D Gonsalves (2003) Papaya: Engineering resistance against Papaya ringspot virus by native, chimeric and synthetic transgenes G Loebenstein G Thottapilly (Eds) Virus and virus like diseases of major crops in developing countries Kluwer Dordrecht 497–518

    Google Scholar 

  • G Fermin V Inglessis C Garboza S Rangel M Dagert D Gonsalves (2004) ArticleTitleTechnology transfer for engineered resistance against PRSV in Venezuelan transgenic papayas Plant Dis 88 516–522 Occurrence Handle10.1094/PDIS.2004.88.5.516

    Article  Google Scholar 

  • D Gonsalves (1994) Papaya diseases caused by viruses RC Ploetz GA Zentmyer WT Nishijima KG Rohrbach HD Ohr (Eds) Compendium of tropical fruit diseases APS Press St. Paul, Minnesota 67–68

    Google Scholar 

  • D Gonsalves G Fermin (2004) Transgenic papaya to control Papaya ringspot virus in Hawaii and technology transfer to other countries P Christou H Klee (Eds) Handbook of plant biotechnology Wiley Chichester 1165–1182

    Google Scholar 

  • TA Hall (1999) ArticleTitleBioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic Acid Symp Ser 41 95–98 Occurrence Handle1:CAS:528:DC%2BD3cXhtVyjs7Y%3D

    CAS  Google Scholar 

  • X Huang (1992) ArticleTitleA contig assembly program based on sensitive detection of fragment overlaps Genomics 14 18–25 Occurrence Handle1427824 Occurrence Handle10.1016/S0888-7543(05)80277-0 Occurrence Handle1:CAS:528:DyaK38Xmt1Oksb0%3D

    Article  PubMed  CAS  Google Scholar 

  • R Jain J Sharma A Sivakumar P Sharma A Byadgi A Verma A Varma (2004) ArticleTitleVariability in the coat protein gene of Papaya ringspot virus isolates from multiple locations in India Arch Virol 149 2435–2442 Occurrence Handle15338325 Occurrence Handle10.1007/s00705-004-0392-x Occurrence Handle1:CAS:528:DC%2BD2cXhtVWjt7bJ

    Article  PubMed  CAS  Google Scholar 

  • D Jensen (1949) ArticleTitlePapaya disease with special reference to papaya ringspot virus Phytopathology 39 191–211

    Google Scholar 

  • Kumar S, Tamura K, Jakobsen I, Nei M (2001) MEGA: molecular evolutionary genetic analysis (software distributed by the authors)

  • RCA Lima MT Souza SuffixJr G Pio-Ribeiro JAA Lima (2002) ArticleTitleSequences of the coat protein gene from Brazilian isolates of Papaya ringspot virus Fitopatol Bras 27 174–180 Occurrence Handle1:CAS:528:DC%2BD38Xkt1OjtLs%3D

    CAS  Google Scholar 

  • KS Ling HY Zhu H Alvizo JS Hu RF Drong JL Slightom D Gonsalves (1997) ArticleTitleThe coat protein gene of grapevine leafroll associated closterovirus-3: cloning, nucleotide sequencing and expression in transgenic plants Arch Virol 142 1101–1116 Occurrence Handle9229001 Occurrence Handle10.1007/s007050050145 Occurrence Handle1:CAS:528:DyaK2sXksF2lsL0%3D

    Article  PubMed  CAS  Google Scholar 

  • D Martin E Rybicki (2000) ArticleTitleRDP: detection of recombination amongst aligned sequences Bioinformatics 16 562–563 Occurrence Handle10980155 Occurrence Handle10.1093/bioinformatics/16.6.562 Occurrence Handle1:CAS:528:DC%2BD3cXnsVelsbc%3D

    Article  PubMed  CAS  Google Scholar 

  • A Muller (1941) ArticleTitleEl reconocimiento de las enfermedades de las plantas cultivadas en Venezuela, 1937–1941 Bol Soc Venez Ci Nat 7 99–113

    Google Scholar 

  • C Napoli C Lemieux R Jorgensen (1990) ArticleTitleIntroduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans Plant Cell 2 279–289 Occurrence Handle12354959 Occurrence Handle10.1105/tpc.2.4.279 Occurrence Handle1:CAS:528:DyaK3cXkvFalu7c%3D

    Article  PubMed  CAS  Google Scholar 

  • Nicholas K, Nicholas H (2000) GeneDoc: a tool for editing and annotating multiple sequence alignments (software distributed by the authors, www.psc.edu/biomed/genedoc)

  • JC Noa-Carrazana D González-de-León BS Ruiz-Castro D Piñero L Silva-Rosales (2006) ArticleTitleDistribution of Papaya ringspot virus and Papaya mosaic virus in papaya plants (Carica papaya) in Mexico Plant Dis 90 1004–1011 Occurrence Handle10.1094/PD-90-1004

    Article  Google Scholar 

  • K Ohshima Y Tomitaka JT Wood Y Minematsu H Kajiyama K Tomimura A Gibbs (2007) ArticleTitlePatterns of recombination in Turnip mosaic virus sequences indicate hotspots of recombination J Gen Virol 88 298–315 Occurrence Handle17170463 Occurrence Handle10.1099/vir.0.82335-0 Occurrence Handle1:CAS:528:DC%2BD2sXmsleluw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • Purcifull D, Edwardson J, Hiebert E, Gonsalves D (1984) Papaya Ringspot Virus. CMI/AAB Descriptions of plant viruses, No. 292. (No. 84 Revis. July 1984), Kew, Surrey, UK

  • J Rozas JC Sánchez-del Barrio X Messeguer R Rozas (2003) ArticleTitleDnaSP, DNA polymorphism analyses by the coalescent and other methods Bioinformatics 19 2496–2497 Occurrence Handle14668244 Occurrence Handle10.1093/bioinformatics/btg359 Occurrence Handle1:CAS:528:DC%2BD3sXpvVSisLo%3D

    Article  PubMed  CAS  Google Scholar 

  • L Silva-Rosales N Becerra-Leor S Ruiz-Castro D Teliz-Ortiz J Noa-Carrazana (2000) ArticleTitleCoat protein sequence comparison of three Mexican isolates of Papaya ringspot virus with other geographical isolates reveal a close relationship to American and Australian isolates Arch Virol 145 835–843 Occurrence Handle10893161 Occurrence Handle10.1007/s007050050676 Occurrence Handle1:CAS:528:DC%2BD3cXksF2msL8%3D

    Article  PubMed  CAS  Google Scholar 

  • J Slightom (1991) ArticleTitleCustom PCR engineering of a plant expression vector Gene 100 251–255 Occurrence Handle2055473 Occurrence Handle10.1016/0378-1119(91)90376-M Occurrence Handle1:CAS:528:DyaK3MXks1KitLw%3D

    Article  PubMed  CAS  Google Scholar 

  • D Stenger DL Seifers R French (2002) ArticleTitlePatterns of polymorphism in Wheat streak mosaic virus: sequence space explored by a clade of closely related viral genotypes rivals that between the most divergent strains Virology 302 58–70 Occurrence Handle12429516 Occurrence Handle10.1006/viro.2001.1569 Occurrence Handle1:CAS:528:DC%2BD38XotlKjtLg%3D

    Article  PubMed  CAS  Google Scholar 

  • P Tennant MH Ahmad D Gonsalves (2002) ArticleTitleTransformation of Carica papaya L. with virus coat protein genes for studies on resistance to Papaya ringspot virus from Jamaica Trop Agric (Trinidad) 79 1–9

    Google Scholar 

  • P Tennant MH Ahmad D Gonsalves (2005) ArticleTitleField resistance of coat protein transgenic papaya to Papaya ringspot virus in Jamaica Plant Dis 89 841–847 Occurrence Handle10.1094/PD-89-0841 Occurrence Handle1:CAS:528:DC%2BD2MXpsFWntbw%3D

    Article  CAS  Google Scholar 

  • Tennant PF (1996) Evaluation of the resistance of coat protein transgenic papaya against papaya ringspot virus isolates and development of transgenic papaya for Jamaica. Ph.D. thesis, Cornell University, Ithaca, NY

  • J Thompson T Gibson F Plewniak F Jeanmougin D Higgins (1997) ArticleTitleThe ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 24 4876–4882 Occurrence Handle10.1093/nar/25.24.4876

    Article  Google Scholar 

  • YP Tian XP Zhu JL Liu XQ Yu J Du J Kreuze XD Li (2007) ArticleTitleMolecular characterization of the 3′-terminal region of Turnip mosaic virus isolates from eastern China J Phytopathol 155 333–341 Occurrence Handle10.1111/j.1439-0434.2007.01236.x Occurrence Handle1:CAS:528:DC%2BD2sXntl2kurw%3D

    Article  CAS  Google Scholar 

  • Y Tomitaka K Ohshima (2006) ArticleTitleA phylogeographical study of the Turnip mosaic virus population in East Asia reveals an “emergent” lineage in Japan Mol Ecol 15 4437–4457 Occurrence Handle17107475 Occurrence Handle10.1111/j.1365-294X.2006.03094.x Occurrence Handle1:CAS:528:DC%2BD2sXmvVSltw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • Vegas A, Trujillo G, Marys E, González A, Fermín G, Cermeli M (2004) El Virus de la mancha anillada de la lechosa en Venezuela: descripción e importancia, medidas de prevención y control. CENIAP Hoy (6)

  • CH Wang SD Yeh (1992) ArticleTitleNucleotide comparison of the 3′-terminal regions of severe, mild and non-papaya infecting strains of Papaya ringspot virus Arch Virol 127 345–354 Occurrence Handle1456896 Occurrence Handle10.1007/BF01309597 Occurrence Handle1:CAS:528:DyaK3sXit1Kqtbo%3D

    Article  PubMed  CAS  Google Scholar 

  • Wang CH, Yeh SD (1997) Divergence and conservation of the genomic RNAs of Taiwan and Hawaii strains of papaya ringspot potyvirus. Arch Virol 142: 271–285; www.ceniap.gov.ve/ceniaphoy/articulos/n6/arti/vegas_a/arti/vegas_a.htm

  • S Yeh F Jan C Chiang T Doong M Chen P Chung H Bau (1992) ArticleTitleComplete nucleotide sequence and genetic organization of Papaya ringspot virus RNA J Gen Virol 73 2531–2541 Occurrence Handle1402799 Occurrence Handle1:CAS:528:DyaK3sXltFSit7g%3D Occurrence Handle10.1099/0022-1317-73-10-2531

    Article  PubMed  CAS  Google Scholar 

  • Young F (1994) The Papaya ringspot virus in Jamaica. In: Proc, Papaya Industry Seminar, Jamaica 1994. IICA, Kingston, Jamaica, pp 39–46

Download references

Author information

Authors and Affiliations

  1. Biotechnology Centre, University of the West Indies, Kingston, Jamaica

    M. Chin & P. Tennant

  2. Faculty of Sciences, Universidad de Los Andes, Mérida, Venezuela

    Y. Rojas, J. Moret & G. Fermin

  3. Department of Life Sciences, University of the West Indies, Kingston, Jamaica

    P. Tennant

  4. Department of Plant Pathology, Cornell University, Geneva, NY, USA

    D. Gonsalves

Authors
  1. M. Chin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Rojas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Moret
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Fermin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Tennant
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. Gonsalves
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chin, M., Rojas, Y., Moret, J. et al. Varying genetic diversity of Papaya ringspot virus isolates from two time-separated outbreaks in Jamaica and Venezuela. Arch Virol 152, 2101–2106 (2007). https://doi.org/10.1007/s00705-007-1035-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-007-1035-9

Keywords

Search

Navigation