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Group A rotavirus genotypes in Germany during 2005/2006

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Summary

During the 2005/2006 winter season a total of 802 group A positive rotavirus specimens of patients from different regions throughout Germany were genotyped. Amplicons from a one-tube RT-PCR were typed by analysis of their (type-specific) size using type-specific primers, fluorescent consensus primers and a capillary sequencer for detection. While G1P[8] was predominant (45.8%), G9P[8] has emerged as the second most frequent genotype combination (37.7%). The distribution of genotypes was heterogeneous, regional frequencies regarding G1 and G9 were ranging from 15.0 to 89.3% and from 7.1 to 67.7%, respectively. Furthermore, a few human rotavirus G10P[6] and G10P[8] infections were observed.

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References

  • MI Adah A Rohwedder OD Olaleyle H Werchau (1997) ArticleTitleNigerian rotavirus serotype G8 could not be typed by PCR due to nucleotide mutation at the 3′ end of the primer binding site Arch Virol 142 1881–1887 Occurrence Handle9672646 Occurrence Handle10.1007/s007050050206 Occurrence Handle1:CAS:528:DyaK2sXmvFyntb4%3D

    Article  PubMed  CAS  Google Scholar 

  • I Banerjee S Ramani B Primrose P Moses M Iturriza-Gomara JJ Gray S Jaffar B Monica JP Muliyil DW Brown MK Estes G Kang (2006) ArticleTitleComparative study of the epidemiology of rotavirus in children from a community-based birth cohort and a hospital in South India J Clin Microbiol 44 2468–2474 Occurrence Handle16825366 Occurrence Handle10.1128/JCM.01882-05

    Article  PubMed  Google Scholar 

  • K Banyai JR Gentsch R Schipp F Jakab E Meleg I Mihaly G Szucs (2005) ArticleTitleDominating prevalence of P[8], G1 and P[8], G9 rotavirus strains among children admitted to hospital between 2000 and 2003 in Budapest, Hungary J Med Virol 76 414–423 Occurrence Handle15902709 Occurrence Handle10.1002/jmv.20372 Occurrence Handle1:CAS:528:DC%2BD2MXlslKhsrs%3D

    Article  PubMed  CAS  Google Scholar 

  • DI Bernstein (2006) ArticleTitleLive attenuated human rotavirus vaccine, Rotarix Semin Pediatr Infect Dis 17 188–194 Occurrence Handle17055369 Occurrence Handle10.1053/j.spid.2006.08.006

    Article  PubMed  Google Scholar 

  • AA Castello MH Arguelles RP Rota A Olthoff B Jiang RI Glass JR Gentsch G Glikmann (2006) ArticleTitleMolecular epidemiology of group A rotavirus diarrhoea among children in Buenos Aires, Argentina, from 1999 to 2003 and emergence of the infrequent genotype G12 J Clin Microbiol 44 2046–2050 Occurrence Handle16757596 Occurrence Handle10.1128/JCM.02436-05 Occurrence Handle1:STN:280:DC%2BD28zktlygsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • HF Clark PA Offit SA Plotkin PM Heaton (2006) ArticleTitleThe new pentavalent rotavirus vaccine composed of bovine (strain WC3) -human rotavirus reassortants Pediatr Infect Dis J 25 577–583 Occurrence Handle16804425 Occurrence Handle10.1097/01.inf.0000220283.58039.b6

    Article  PubMed  Google Scholar 

  • N Cook J Bridger K Kendall MI Gomara L El-Attar J Gray (2004) ArticleTitleThe zoonotic potential of rotavirus J Infect 48 289–302 Occurrence Handle15066329 Occurrence Handle10.1016/j.jinf.2004.01.018

    Article  PubMed  Google Scholar 

  • S De Grazia S Ramirez GM Giammanco C Colomba V Martella C Lo Biundo R Mazzola S Arista (2007) ArticleTitleDiversity of human rotaviruses detected in Sicily, Italy, over a 5-year period (2001–2005) Arch Virol 152 833–837 Occurrence Handle17160368 Occurrence Handle10.1007/s00705-006-0874-0 Occurrence Handle1:CAS:528:DC%2BD2sXjt1KrurY%3D

    Article  PubMed  CAS  Google Scholar 

  • B De Vos T Vesikari AC Linhares B Salinas I Perez-Schael GM Ruiz-Palacios L Guerrero Mde KB Phua A Delem K Hardt (2004) ArticleTitleA rotavirus vaccine for prophylaxis of infants against rotavirus gastroenteritis Pediatr Infect Dis J 23 S179–S182 Occurrence Handle15502699 Occurrence Handle10.1097/01.inf.0000142370.16514.4a

    Article  PubMed  Google Scholar 

  • U Desselberger M Iturriza-Gomara JJ Gray (2001) ArticleTitleRotavirus epidemiology and surveillance Novartis Found Symp 238 125–147 Occurrence Handle11444024 Occurrence Handle1:STN:280:DC%2BD38%2FhvV2luw%3D%3D

    PubMed  CAS  Google Scholar 

  • U Desselberger J Wolleswinkel-van den Bosch J Mrukowicz C Rodrigo C Giaquinto T Vesikari (2006) ArticleTitleRotavirus types in Europe and their significance for vaccination Pediatr Infect Dis J 25 S30–S41 Occurrence Handle16397427 Occurrence Handle10.1097/01.inf.0000197707.70835.f3

    Article  PubMed  Google Scholar 

  • S Diwakarla R Clark EA Palombo (2002) ArticleTitleExpanding distribution of human serotype G6 rotaviruses in Australia Microbiol Immunol 46 499–502 Occurrence Handle12222938 Occurrence Handle1:CAS:528:DC%2BD38Xmtl2mt7s%3D

    PubMed  CAS  Google Scholar 

  • B Ehlken B Laubereau W Karmaus G Petersen A Rohwedder J Forster (2002) ArticleTitleProspective population-based study on rotavirus disease in Germany Acta Paediatr 91 769–775 Occurrence Handle12200901 Occurrence Handle10.1080/08035250213227 Occurrence Handle1:STN:280:DC%2BD38vksFyrsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • JR Gentsch RI Glass P Woods V Gouvea M Gorziglia J Flores BK Das MK Bhan (1992) ArticleTitleIdentification of group A rotavirus gene 4 types by polymerase chain reaction J Clin Microbiol 30 1365–1373 Occurrence Handle1320625 Occurrence Handle1:CAS:528:DyaK3sXnsVCiug%3D%3D

    PubMed  CAS  Google Scholar 

  • JR Gentsch AR Laird B Bielfelt DD Griffin K Banyai M Ramachandran V Jain NA Cunliffe O Nakagomi CD Kirkwood TK Fischer UD Parashar JS Bresee B Jiang RI Glass (2005) ArticleTitleSerotype diversity and reassortment between human and animal rotavirus strains: implications for rotavirus vaccine programs J Infect Dis 192 IssueIDSuppl 1 S146–S159 Occurrence Handle16088798 Occurrence Handle10.1086/431499

    Article  PubMed  Google Scholar 

  • MI Gomara D Cubitt U Desselberger J Gray (2001) ArticleTitleAmino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype J Clin Microbiol 39 3796–3798 Occurrence Handle11574622 Occurrence Handle10.1128/JCM.39.10.3796-3798.2001 Occurrence Handle1:CAS:528:DC%2BD3MXnslKhsLg%3D

    Article  PubMed  CAS  Google Scholar 

  • V Gouvea RI Glass P Woods K Taniguchi HF Clark B Forrester ZY Fang (1990) ArticleTitlePolymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens J Clin Microbiol 28 276–282 Occurrence Handle2155916 Occurrence Handle1:CAS:528:DyaK3cXhvFClsbY%3D

    PubMed  CAS  Google Scholar 

  • M Iturriza-Gomara D Cubitt D Steele J Green D Brown G Kang U Desselberger J Gray (2000) ArticleTitleCharacterisation of rotavirus G9 strains isolated in the UK between 1995 and 1998 J Med Virol 61 510–517 Occurrence Handle10897071 Occurrence Handle10.1002/1096-9071(200008)61:4<510::AID-JMV15>3.0.CO;2-Q Occurrence Handle1:CAS:528:DC%2BD3cXlsVOis7s%3D

    Article  PubMed  CAS  Google Scholar 

  • M Iturriza-Gomara J Green DW Brown U Desselberger JJ Gray (2000) ArticleTitleDiversity within the VP4 gene of rotavirus P[8] strains: implications for reverse transcription-PCR genotyping J Clin Microbiol 38 898–901 Occurrence Handle10655412 Occurrence Handle1:CAS:528:DC%2BD3cXht1ejurk%3D

    PubMed  CAS  Google Scholar 

  • M Iturriza-Gomara J Green DW Brown M Ramsay U Desselberger JJ Gray (2000) ArticleTitleMolecular epidemiology of human group A rotavirus infections in the United Kingdom between 1995 and 1998 J Clin Microbiol 38 4394–4401 Occurrence Handle11101570 Occurrence Handle1:STN:280:DC%2BD3M%2FnsVCgsg%3D%3D

    PubMed  CAS  Google Scholar 

  • M Iturriza-Gomara B Isherwood U Desselberger J Gray (2001) ArticleTitleReassortment in vivo: driving force for diversity of human rotavirus strains isolated in the United Kingdom between 1995 and 1999 J Virol 75 3696–3705 Occurrence Handle11264359 Occurrence Handle10.1128/JVI.75.8.3696-3705.2001 Occurrence Handle1:CAS:528:DC%2BD3MXisVSmsrs%3D

    Article  PubMed  CAS  Google Scholar 

  • M Iturriza-Gomara G Kang J Gray (2004) ArticleTitleRotavirus genotyping: keeping up with an evolving population of human rotaviruses J Clin Virol 31 259–265 Occurrence Handle15494266 Occurrence Handle10.1016/j.jcv.2004.04.009 Occurrence Handle1:CAS:528:DC%2BD2cXos1Cju7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • C Kirkwood N Bogdanovic-Sakran E Palombo P Masendycz H Bugg G Barnes R Bishop (2003) ArticleTitleGenetic and antigenic characterization of rotavirus serotype G9 strains isolated in Australia between 1997 and 2001 J Clin Microbiol 41 3649–3654 Occurrence Handle12904370 Occurrence Handle10.1128/JCM.41.8.3649-3654.2003 Occurrence Handle1:CAS:528:DC%2BD3sXnt1Slu7k%3D

    Article  PubMed  CAS  Google Scholar 

  • J Matthijnssens M Rahman V Martella Y Xuelei S De Vos K De Leener M Ciarlet C Buonavoglia M Van Ranst (2006) ArticleTitleFull genomic analysis of human rotavirus strain B4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission J Virol 80 3801–3810 Occurrence Handle16571797 Occurrence Handle10.1128/JVI.80.8.3801-3810.2006 Occurrence Handle1:CAS:528:DC%2BD28Xjs1SrtLg%3D

    Article  PubMed  CAS  Google Scholar 

  • L Maunula CH von Bonsdorff (2002) ArticleTitleFrequent reassortments may explain the genetic heterogeneity of rotaviruses: analysis of Finnish rotavirus strains J Virol 76 11793–11800 Occurrence Handle12414921 Occurrence Handle10.1128/JVI.76.23.11793-11800.2002 Occurrence Handle1:CAS:528:DC%2BD38Xos12qsrY%3D

    Article  PubMed  CAS  Google Scholar 

  • DY Oh G Gaedicke E Schreier (2003) ArticleTitleViral agents of acute gastroenteritis in German children: prevalence and molecular diversity J Med Virol 71 82–93 Occurrence Handle12858413 Occurrence Handle10.1002/jmv.10449 Occurrence Handle1:CAS:528:DC%2BD3sXmslOrtLc%3D

    Article  PubMed  CAS  Google Scholar 

  • EA Palombo (2002) ArticleTitleGenetic analysis of Group A rotaviruses: evidence for interspecies transmission of rotavirus genes Virus Genes 24 11–20 Occurrence Handle11928984 Occurrence Handle10.1023/A:1014073618253 Occurrence Handle1:CAS:528:DC%2BD38XisVShtLs%3D

    Article  PubMed  CAS  Google Scholar 

  • UD Parashar CJ Gibson JS Bresse RI Glass (2006) ArticleTitleRotavirus and severe childhood diarrhoea Emerg Infect Dis 12 304–306 Occurrence Handle16494759

    PubMed  Google Scholar 

  • SH Park LJ Saif C Jeong GK Lim SI Park HH Kim SJ Park YJ Kim JH Jeong MI Kang KO Cho (2006) ArticleTitleMolecular characterization of novel G5 bovine rotavirus strains J Clin Microbiol 44 4101–4112 Occurrence Handle16928963 Occurrence Handle10.1128/JCM.01196-06 Occurrence Handle1:CAS:528:DC%2BD28XhtlSnu7jE

    Article  PubMed  CAS  Google Scholar 

  • JB Pesavento SE Crawford MK Estes BV Prasad (2006) ArticleTitleRotavirus proteins: structure and assembly Curr Top Microbiol Immunol 309 189–219 Occurrence Handle16913048 Occurrence Handle1:CAS:528:DC%2BD28Xos1yms7s%3D Occurrence Handle10.1007/3-540-30773-7_7

    Article  PubMed  CAS  Google Scholar 

  • M Rahman J Matthijnssens T Goegebuer K De Leener L Vanderwegen I van der Donck L Van Hoovels S De Vos T Azim M Van Ranst (2005) ArticleTitlePredominance of rotavirus G9 genotype in children hospitalized for rotavirus gastroenteritis in Belgium during 1999–2003 J Clin Virol 33 1–6 Occurrence Handle15797358 Occurrence Handle10.1016/j.jcv.2004.09.020

    Article  PubMed  Google Scholar 

  • N Reidy F O’Halloran S Fanning B Cryan H O’Shea (2005) ArticleTitleEmergence of G3 and G9 rotavirus and increased incidence of mixed infections in the southern region of Ireland 2001–2004 J Med Virol 77 571–578 Occurrence Handle16254970 Occurrence Handle10.1002/jmv.20494 Occurrence Handle1:CAS:528:DC%2BD2MXht1els7jI

    Article  PubMed  CAS  Google Scholar 

  • RKI SurvStat. http://www3.rki.de/SurvStat

  • E Rubilar-Abreu KO Hedlund L Svensson C Mittelholzer (2005) ArticleTitleSerotype G9 rotavirus infections in adults in Sweden J Clin Microbiol 43 1374–1376 Occurrence Handle15750111 Occurrence Handle10.1128/JCM.43.3.1374-1376.2005

    Article  PubMed  Google Scholar 

  • B Salinas I Perez Schael AC Linhares GM Ruiz Palacios ML Guerrero JP Yarzabal Y Cervantes S Costa Clemens S Damaso K Hardt B De Vos (2005) ArticleTitleEvaluation of safety, immunogenicity and efficacy of an attenuated rotavirus vaccine, RIX4414: a randomized, placebo-controlled trial in Latin American infants Pediatr Infect Dis J 24 807–816 Occurrence Handle16148848 Occurrence Handle10.1097/01.inf.0000178294.13954.a1

    Article  PubMed  Google Scholar 

  • A Sanchez-Fauquier V Montero S Moreno M Sole J Colomina M Iturriza-Gomara A Revilla I Wilhelmi J Gray (2006) ArticleTitleHuman rotavirus G9 and G3 as major cause of diarrhoea in hospitalized children, Spain Emerg Infect Dis 12 1536–1541 Occurrence Handle17176568

    PubMed  Google Scholar 

  • N Santos RC Lima CM Nozawa RE Linhares V Gouvea (1999) ArticleTitleDetection of porcine rotavirus type G9 and of a mixture of types G1 and G5 associated with Wa-like VP4 specificity: evidence for natural human-porcine genetic reassortment J Clin Microbiol 37 2734–2736 Occurrence Handle10405435 Occurrence Handle1:CAS:528:DyaK1MXltVSqsrY%3D

    PubMed  CAS  Google Scholar 

  • N Santos Y Hoshino (2005) ArticleTitleGlobal distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine Rev Med Virol 15 29–56 Occurrence Handle15484186 Occurrence Handle10.1002/rmv.448

    Article  PubMed  Google Scholar 

  • A Steyer M Poljsak-Prijatelj D Barlic-Maganja T Bufon J Marin (2005) ArticleTitleThe emergence of rotavirus genotype G9 in hospitalised children in Slovenia J Clin Virol 33 7–11 Occurrence Handle15797359 Occurrence Handle10.1016/j.jcv.2004.09.021 Occurrence Handle1:STN:280:DC%2BD2M7msFKmsg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • V Varghese S Das NB Singh K Kojima SK Bhattacharya T Krishnan N Kobayashi TN Naik (2004) ArticleTitleMolecular characterization of a human rotavirus reveals porcine characteristics in most of the genes including VP6 and NSP4 Arch Virol 149 155–172 Occurrence Handle14689281 Occurrence Handle10.1007/s00705-003-0199-1 Occurrence Handle1:CAS:528:DC%2BD3sXpvVKqtrs%3D

    Article  PubMed  CAS  Google Scholar 

  • MA Widdowson GJ van Doornum WH van der Poel AS de Boer R van de Heide U Mahdi P Haanen JL Kool M Koopmans (2002) ArticleTitleAn outbreak of diarrhoea in a neonatal medium care unit caused by a novel strain of rotavirus: investigation using both epidemiologic and microbiological methods Infect Control Hosp Epidemiol 23 665–670 Occurrence Handle12452294 Occurrence Handle10.1086/501991

    Article  PubMed  Google Scholar 

  • M Yoshinaga TG Phan TA Nguyen H Yan F Yagyu S Okitsu WE Muller H Ushijima (2006) ArticleTitleChanging distribution of group A rotavirus G-types and genetic analysis of G9 circulating in Japan Arch Virol 151 183–192 Occurrence Handle16086098 Occurrence Handle10.1007/s00705-005-0604-z Occurrence Handle1:CAS:528:DC%2BD2MXhtlWrsL%2FI

    Article  PubMed  CAS  Google Scholar 

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Marques, A., Diedrich, S., Huth, C. et al. Group A rotavirus genotypes in Germany during 2005/2006. Arch Virol 152, 1743–1749 (2007). https://doi.org/10.1007/s00705-007-0998-x

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