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Complete nucleotide sequences and genome characterization of a novel double-stranded RNA virus infecting Rosa multiflora

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Abstract

The three double-stranded (ds) RNAs were detected in Rosa multiflora plants showing rose spring dwarf (RSD) symptoms. Northern blot analysis revealed three dsRNAs in preparations of both dsRNA and total RNA from R. multiflora plants. The complete sequences of the dsRNAs (referred to as dsRNA 1, dsRNA 2 and dsRNA 3) were determined based on a combination of shotgun cloning of dsRNA cDNAs and reverse transcription-polymerase chain reaction (RT-PCR). The largest dsRNA (dsRNA 1) was 1,762 bp long with a single open reading frame (ORF) that encoded a putative polypeptide containing 479 amino acid residues with a molecular mass of 55.9 kDa. This polypeptide contains amino acid sequence motifs conserved in the RNA-dependent RNA polymerases (RdRp) of members of the family Partitiviridae. Both dsRNA 2 (1,475 bp) and dsRNA 3 (1,384 bp) contained single ORFs, encoding putative proteins of unknown function. The 5′ untranslated regions (UTR) of all three segments shared regions of high sequence homology. Phylogenetic analysis using the RdRp sequences of the various partitiviruses revealed that the new sequences would constitute the genome of a virus in family Partitiviridae. This virus would cluster with Fragaria chiloensis cryptic virus and Raphanus sativus cryptic virus 2. We suggest that the three dsRNA segments constitute the genome of a novel cryptic virus infecting roses; we propose the name Rosa multiflora cryptic virus (RMCV). Detection primers were developed and used for RT-PCR detection of RMCV in rose plants.

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Acknowledgments

N. Salem is grateful to the Fulbright Commission for a postdoctoral fellowship. We would like to acknowledge Dr. Steven Daubert (Department of Plant Pathology, UC Davis) for invaluable comments and suggestion on the manuscript, Dr. Anne-Laure Moyne (Department of Plant Pathology, UC Davis), Susan T. Sim, Judy Lee and Mike Cunningham (Foundation Plant Services, UC Davis) for their technical support. This project was also partially supported by funding from the Garden Rose Council.

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  1. Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA, 95616, USA

    Nidá M. Salem, Deborah A. Golino, Bryce W. Falk & Adib Rowhani

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  1. Nidá M. Salem
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  2. Deborah A. Golino
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  3. Bryce W. Falk
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  4. Adib Rowhani
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Correspondence to Adib Rowhani.

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Salem, N.M., Golino, D.A., Falk, B.W. et al. Complete nucleotide sequences and genome characterization of a novel double-stranded RNA virus infecting Rosa multiflora . Arch Virol 153, 455–462 (2008). https://doi.org/10.1007/s00705-007-0008-3

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  • DOI: https://doi.org/10.1007/s00705-007-0008-3

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