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Tumor necrosis factor alpha is not a pathogenic determinant in acute lethal encephalitis induced by a highly neurovirulent strain of mouse hepatitis virus

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Abstract

To investigate the role of tumor necrosis factor alpha (TNFα) in the pathogenesis of acute viral encephalitis, TNFα-deficient mice were infected with a highly neurovirulent strain of mouse hepatitis virus, JHM, and compared with JHM-infected C57BL/6 mice as controls. All the JHM-infected mice had succumbed to infection by 6 days postinfection. The virus replication kinetics, histopathological changes and mRNA expression levels of proinflammatory cytokines in the brain did not differ between TNFα-deficient and control C57BL/6 mice. These results suggest that TNFα is not a pathogenic determinant in JHM-induced acute lethal encephalitis.

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Acknowledgments

This study was supported by Grant-In-Aid Scientific Research (C) No. 10660282 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Kazuya Shirato & Takahide Taniguchi

  2. Research Team for Paratuberculosis, National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki, 305-0856, Japan

    Eiichi Momotani

  3. Research Center for Prion Diseases, National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki, 305-0856, Japan

    Masuhiro Takata

  4. Department of Molecular Biology and Immunology, National Institute of Agrobiological Sciences, Kannnondai, Tsukuba, Ibaraki, 305-8602, Japan

    Kenji Sekikawa

  5. Laboratory of Veterinary Microbiology, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan

    Takahide Taniguchi

  6. Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Musashimurayama, Tokyo, 208-0011, Japan

    Kazuya Shirato

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  1. Kazuya Shirato
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  2. Eiichi Momotani
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  3. Masuhiro Takata
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Correspondence to Takahide Taniguchi.

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Shirato, K., Momotani, E., Takata, M. et al. Tumor necrosis factor alpha is not a pathogenic determinant in acute lethal encephalitis induced by a highly neurovirulent strain of mouse hepatitis virus. Arch Virol 153, 549–553 (2008). https://doi.org/10.1007/s00705-007-0006-5

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  • DOI: https://doi.org/10.1007/s00705-007-0006-5

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