Skip to main content

Advertisement

SpringerLink
Log in

Molecular epidemiology of wild poliovirus type 1 circulation in West and Central Africa, from 1997 to 1999, using genotyping with a restriction fragment length polymorphism assay

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Virological surveillance is an important element in the Polio Eradication Initiative to provide information rapidly about circulating wild polioviruses. Molecular tools have been developed to identify the serotype of the poliovirus strains and whether they are of vaccine or wild origin (intratypic differentiation) and to perform the molecular epidemiology of wild strains. The main objective of this study was to show that restriction fragment length polymorphism (RFLP) is a tool that can be used for molecular epidemiology of wild polioviruses. This is retrospective study of poliovirus type 1 strains received at the Institut Pasteur of Bangui (IPB), a WHO Regional Reference Laboratory for Africa, since 1994. We describe our experience with isolates from Western and Central Africa and show a positive correlation between the genotypes as determined by sequencing the gene for the VP1 capsid protein and the RFLP patterns. Although genomic sequencing is the gold standard method for detailed molecular epidemiology analysis of poliovirus isolates, these results show that RFLP is a potentially valuable tool for molecular epidemiological analysis of poliovirus type 1 strains: it could be used by many laboratories as a rapid method for ITD and genotype screening where sequencing capacity is not readily available.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Anonymous (2006) Imported vaccine-associated paralytic poliomyelitis-Unated States, 2005. MMWR Morb Mortal Wkly Rep 55:97–99

    Google Scholar 

  2. Anonymous (2006) Resurgence of Wild Poliovirus Type 1 Transmission and Consequences of Importation-21 Countries, 2002–2005. MMWR Morb Mortal Wkly Rep 55:145–150

    Google Scholar 

  3. Balanant J, Guillot S, Candrea A, Delpeyroux F, Crainic R (1991) The natural genomic variability of poliovirus analyzed by a restriction frangment length polymorphism assay. Virology 184:645–654

    Article  PubMed  CAS  Google Scholar 

  4. Gouandjika I, Legall P, Kelembo EK, Mathiot CC, Morvan JM (1995) Change in the type of wild poliovirus circulating in the Central African Republic between 1991 and 1994. Trans R Soc Trop Med Hyg 89:576

    Article  PubMed  CAS  Google Scholar 

  5. Kew O, Morris-Glasgow V, Landaverde M, Burns C, Shaw J, Garib Z, Andre J, Blackman E, Freeman CJ, Jorba J, Sutter R, Tambini G, Venczel L, Pedreira C, Laender F, Shimizu H, Yoneyama T, Miyamura T, van Der Avoort H, Oberste MS, Kilpatrick D, Cochi S, Pallansch M, de Quadros C (2002) Outbreak of poliomyelitis in Hispaniola associated with circulating type 1 vaccine-derived poliovirus. Science 296:356–359

    Article  PubMed  CAS  Google Scholar 

  6. Kew OM, Mulders MN, Lipskaya GY, da Silva EE, Pallansch MA (1995) Molecular epidemiology of polioviruses. In: Kew ON (ed) Molecular Epidemiology. Seminars in Virology. Academic, New York, pp 401–414

    Google Scholar 

  7. Kew OM, Yang CF, Nottay B, Pallansch MA (1993) The role of virologic surveillance in the global initiative to eradicate poliomyelitis. In: Kurstak E (ed) Control of virus diseases, 2nd edn., Marcel Dekker, New York, pp 215–245

    Google Scholar 

  8. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  9. Liu HM, Zheng DP, Zhang LB, Oberste MS, Pallansch MA, Kew OM (2000) Molecular evolution of a type 1 wild-vaccine poliovirus recombinant during widespread circulation in China. J Virol 74:11153–11161

    Article  PubMed  CAS  Google Scholar 

  10. Morvan JM, Chezzi C, Gouandjika I, Reimerink JH, van der Avoort HG (1997) The molecular epidemiology of type 1 poliovirus in Central African Republic. J Gen Virol 78(Pt 3):591–599

    PubMed  CAS  Google Scholar 

  11. Moss EG, O’Neill R, Racaniello VRM. o. a. s. o. a. a. p. t. s. J. V. (1989) Mapping of atttenuating sequences of an avirulent poliovirus type 2 strain. J Virol 63:1884–1890

    Google Scholar 

  12. Rico-Hesse R, Pallansch MA, Nottay BK, Kew OM (1987) Geographic distribution of wild poliovirus type 1 genotypes. Virology 160:311–322

    Article  PubMed  CAS  Google Scholar 

  13. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  14. van der Avoort HG, Hull BP, Hovi T, Pallansch MA, Kew OM, Crainic R, Wood DJ, Mulders MN, van Loon AM (1995) Comparative study of five methods for intratypic differentiation of polioviruses. J Clin Microbiol 33:2562–2566

    PubMed  Google Scholar 

  15. Ward NA, Kim-Farley RJ, Milstein JB, Hull HF, Torel C, de Quadros C (1993) Poliomyelitis eradication. Expanded programme on immunization of the World Health Organization. In: Kurstak E (ed) Control of virus diseases, 2nd edn. Marcel Dekker, New York, pp 247–266

    Google Scholar 

  16. WHO (1997) Manual for the virological investigation of polio. In WHO/EPI/GEN/97.01. World Health Organization

  17. WHO (2000) Global polio eradication initiative. Strategic plan 2001–2005. WHO/POLIO/00.05. World Health Organization, Geneva

  18. WorldHealthAssembly (1988) Global eradication of poliomyelitis by the year 2000: resolution of the 41st World Health Assembly. World Health Organization (WHA resolution no. 41.28), Geneva, Switzerland

  19. Wright PF, Kim-Farley RJ, de Quadros CA, Robertson SE, Scott RM, Ward NA, Henderson RH (1991) Strategies for the global eradication of poliomyelitis by the year 2000. N Engl J Med 325:1774–1779

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank Antoine Talarmin for useful discussions, Arthur Mazitchi and Jean Fandema for performing the ITD testing of the strains, Jacqueline Quay and Jane Iber for sequencing some of the isolates, and Paul Chenoweth and Qi Chen for assistance with data organization and analysis. Strains from Cameroon were sent by Emmanuel Tina-Abada, from Centre Pasteur de Yaounde, in 2002. This work was supported in part by a grant from the WHO and by the French Ministry for Research. It was also supported by grants from the Direction of Foreign Affairs and from the Transverse Research Programs (PTR120) of the Pasteur Institute and from the French Ministry of Foreign Affairs (FSP 2001-168). The findings and conclusions in this report are those of the authors and do not necessarily reflect the views of the funding agency.

Author information

Authors and Affiliations

  1. Institut Pasteur de Bangui, PO Box BP 923, Bangui, Central African Republic

    Ionela Gouandjika-Vasilache & Didier Ménard

  2. National Institute for Communicable Diseases, Private Bag X4, Sandringham, 2131, South Africa

    Nicksy Gumede

  3. Centers for Disease Control and Prevention, Atlanta, GA, 30333, USA

    Cara C. Burns, Mark A. Pallansch & Olen M. Kew

  4. Institut Pasteur, B.P 220, Dakar, Senegal

    Annick Dosseh

  5. Institut Pasteur, B.P 490, Abidjan 01, Côte d’Ivoire

    Chantal Akoua-Koffi

  6. Institut Pasteur, Cedex 15, 75724, Paris, France

    Sophie Guillot & Francis Delpeyroux

  7. Institut Pasteur de Madagascar, B.P.1274, 101, Antananarivo, Madagascar

    Didier Ménard

  8. WHO, Bobo-Dioulasso, Burkina Faso

    Annick Dosseh

Authors
  1. Ionela Gouandjika-Vasilache
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cara C. Burns
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nicksy Gumede
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sophie Guillot
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Didier Ménard
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Annick Dosseh
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chantal Akoua-Koffi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Mark A. Pallansch
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Olen M. Kew
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Francis Delpeyroux
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ionela Gouandjika-Vasilache.

Additional information

The GenBank accession numbers of the sequences reported in this paper are AY618419 to AY618458.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gouandjika-Vasilache, I., Burns, C.C., Gumede, N. et al. Molecular epidemiology of wild poliovirus type 1 circulation in West and Central Africa, from 1997 to 1999, using genotyping with a restriction fragment length polymorphism assay. Arch Virol 153, 409–416 (2008). https://doi.org/10.1007/s00705-007-0001-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-007-0001-x

Keywords

Search

Navigation