Archives of Virology

, 152:1027 | Cite as

Detection strategies of tick-borne encephalitis virus in Swedish Ixodes ricinus reveal evolutionary characteristics of emerging tick-borne flaviviruses

  • W. Melik
  • A. S. Nilsson
  • M. Johansson
Brief Report

Summary.

The flaviviral tick-borne encephalitis virus (TBEV) is a human pathogen having significant impact on public health. The geographical distribution of TBEV and TBEV-like viruses is increasing, which makes it important to characterise the natural virus populations. Here we present four RT-PCR strategies designed for detection of broad types of tick-borne flaviviruses. Sequence information on more than 32% of a TBEV genome was generated from a small pool of ticks collected in the Stockholm archipelago on the island of Torö. The sequences were characterised and compared with those of other tick-borne flaviviruses, which classified the virus as Western European TBEV.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • W. Melik
    • 1
    • 2
  • A. S. Nilsson
    • 2
  • M. Johansson
    • 1
  1. 1.School of Life SciencesSödertörns University CollegeHuddingeSweden
  2. 2.Department of Genetics, Microbiology and ToxicologyStockholm UniversityStockholmSweden

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