Detection strategies of tick-borne encephalitis virus in Swedish Ixodes ricinus reveal evolutionary characteristics of emerging tick-borne flaviviruses
- 123 Downloads
The flaviviral tick-borne encephalitis virus (TBEV) is a human pathogen having significant impact on public health. The geographical distribution of TBEV and TBEV-like viruses is increasing, which makes it important to characterise the natural virus populations. Here we present four RT-PCR strategies designed for detection of broad types of tick-borne flaviviruses. Sequence information on more than 32% of a TBEV genome was generated from a small pool of ticks collected in the Stockholm archipelago on the island of Torö. The sequences were characterised and compared with those of other tick-borne flaviviruses, which classified the virus as Western European TBEV.
- Burke, DS, Monath, TP 2001Fields virology4Lippincott-RavenPhiladelphiaGoogle Scholar
- Gritsun, TS, Venugopal, K, Zanotto, PM, Mikhailov, MV, Sall, AA, Holmes, EC, Polkinghorne, I, Frolova, TV, Pogodina, VV, Lashkevich, VA, Gould, EA 1997Complete sequence of two tick-borne flaviviruses isolated from Siberia and the UK: analysis and significance of the 5′ and 3′ UTRsVirus Res492739CrossRefPubMedGoogle Scholar
- Lindenbach, BD, Rice, CM 2001Fields virology4Lippincott-RavenPhiladelphiaGoogle Scholar
- Swofford DL (2000) Phylogenetic analysis using parsimony and other methods. Sunderland, MA, USAGoogle Scholar