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Cucumber mosaic virus as a presentation system for a double hepatitis C virus-derived epitope

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Summary.

Chimeric plant viruses are emerging as promising vectors for use in innovative vaccination strategies. In this context, cucumber mosaic virus (CMV) has proven to be a suitable carrier of the hepatitis C virus (HCV)-derived R9 mimotope. In the present work, a new chimeric CMV, expressing on its surface the HCV-derived R10 mimotope, was produced but lost the insert after the first passage on tobacco. A comparative analysis between R10- and R9-CMV properties indicated that R9-CMV stability was related to structural features typical of the foreign insert. Thus, in order to combine high virus viability with strong immuno-stimulating activity, we doubled R9 copies on each of the 180 coat protein (CP) subunits of CMV. One of the chimeras produced by this approach (2R9-CMV) was shown to systemically infect the host, stably maintaining both inserts. Notably, it was strongly recognized by sera of HCV-infected patients and, as compared with R9-CMV, displayed an enhanced ability to stimulate lymphocyte IFN-γ production. The high immunogen levels achievable in plants or fruits infected with 2R9-CMV suggest that this chimeric form of CMV may be useful in the development of oral vaccines against HCV.

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Authors and Affiliations

  1. Department of Biology, Plant Protection and Agrobiotechnology, University of Basilicata, Potenza, Italy

    M. Nuzzaci, A. Vitti, M. Lapelosa, A. Natilla & P. Piazzolla

  2. Department of Internal Medicine, Immunology and Infectious Diseases, Section of Internal Medicine, University of Bari, Bari, Italy

    G. Piazzolla, C. Tortorella, I. Stella & S. Antonaci

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  1. M. Nuzzaci
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  2. G. Piazzolla
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  3. A. Vitti
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  4. M. Lapelosa
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  5. C. Tortorella
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  7. A. Natilla
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  8. S. Antonaci
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  9. P. Piazzolla
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Nuzzaci, M., Piazzolla, G., Vitti, A. et al. Cucumber mosaic virus as a presentation system for a double hepatitis C virus-derived epitope. Arch Virol 152, 915–928 (2007). https://doi.org/10.1007/s00705-006-0916-7

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