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Characterisation of several heterogeneous species of defective RNAs derived from RNA 3 of cucumber mosaic virus

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Summary.

Preparations of double-stranded RNAs (dsRNAs) extracted from Nicotiana tabacum cv Xanthi plants infected with a subgroup IB isolate of Cucumber mosaic virus (CMV) were found to contain a heterogeneous population of defective RNAs (D-RNAs) derived from RNA 3. Characterised D-RNAs ranged in size from 1.5 to 1.9 kb and were derived either by a single in-frame deletion within the 3a or 3b genes or by means of double in-frame deletions within both genes. Also, northern blot hybridisation showed two other types of RNA derived from RNA 3: (a) RNA species of ca. 0.7 kb containing the 3′-terminus but lacking the 5′-terminus, which could be 3′-coterminal subgenomic of D-RNAs derived from the 3b gene and (b) RNA species of unknown origin of ca. 0.8 kb containing the 5′-terminus but lacking the 3′-terminus.

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References

  • MA Ayllón C López J Navas-Castillo M Mawassi WO Dawson J Guerri R Flores P Moreno (1999) ArticleTitleNew defective RNAs from citrus tristeza virus: evidence for a replicase-driven template switching mechanism in their generation J Gen Virol 80 817–821 Occurrence Handle10092024

    PubMed  Google Scholar 

  • G Brigneti O Voinnet WX Li LH Ji SW Ding DC Baulcombe (1998) ArticleTitleViral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana EMBO J 17 6739–6674 Occurrence Handle9822616 Occurrence Handle10.1093/emboj/17.22.6739 Occurrence Handle1:CAS:528:DyaK1cXnvF2jtLw%3D

    Article  PubMed  CAS  Google Scholar 

  • A Crescenzi L Barbarossa D Gallitelli GP Martelli (1993) ArticleTitleCucumber mosaic cucumovirus populations in Italy under natural epidemic conditions and after a satellite-mediated protection test Plant Dis 77 28–33 Occurrence Handle1:CAS:528:DyaK3sXksVGgtLg%3D Occurrence Handle10.1094/PD-77-0028

    Article  CAS  Google Scholar 

  • J Devereux P Haeberli O Smithies (1984) ArticleTitleA comprehensive set of sequence analysis programmes for the VAX Nucleic Acids Res 12 387–395 Occurrence Handle6546423 Occurrence Handle10.1093/nar/12.1Part1.387 Occurrence Handle1:CAS:528:DyaL2cXot1eqtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • SW Ding BJ Anderson HR Haase RH Symons (1994) ArticleTitleNew overlapping gene encoded by the cucumber mosaic virus genome Virology 198 593–601 Occurrence Handle8291242 Occurrence Handle10.1006/viro.1994.1071 Occurrence Handle1:CAS:528:DyaK2cXhsFGhu7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • D Gallitelli C Vovias A di Franco C Cariddi A Crescenzi A Ragozzino (1990) ArticleTitleCucumber mosaic virus as a major [factor] responsible for tomato epidemics in southern Italy Acta Hortic 277 241–245

    Google Scholar 

  • MV Graves MJ Roossinck (1995) ArticleTitleCharacterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus J Virol 69 4746–4751 Occurrence Handle7609040 Occurrence Handle1:CAS:528:DyaK2MXmvFWgsrw%3D

    PubMed  CAS  Google Scholar 

  • C Hernández JA Carette DJF Brown JF Bol (1996) ArticleTitleSerial passage of tobacco rattle virus under different selection conditions results in deletion of structural and nonstructural genes in RNA 2 J Virol 70 4933–4940 Occurrence Handle8763997

    PubMed  Google Scholar 

  • BI Hillman JC Carrington TJ Morris (1987) ArticleTitleA defective interfering RNA that contains a mosaic of a plant virus genome Cell 51 427–433 Occurrence Handle3664641 Occurrence Handle10.1016/0092-8674(87)90638-6 Occurrence Handle1:CAS:528:DyaL1cXhtlGg

    Article  PubMed  CAS  Google Scholar 

  • C Jordá A Alfaro MA Aranda E Moriones F García-Arenal (1992) ArticleTitleEpidemic of cucumber mosaic virus plus satellite RNA in tomatoes in Eastern Spain Plant Dis 76 363–366 Occurrence Handle10.1094/PD-76-0363

    Article  Google Scholar 

  • JM Kaper HE Waterworth (1977) ArticleTitleCucumber mosaic virus associated RNA 5: causal agent for tomato necrosis Science 196 429–431 Occurrence Handle10.1126/science.196.4288.429 Occurrence Handle1:CAS:528:DyaE2sXhs1yjtbo%3D Occurrence Handle17776951

    Article  CAS  PubMed  Google Scholar 

  • IB Kaplan KC Lee T Canto SM Won P Palukaitis (2004) ArticleTitleHost-specific encapsidation of a defective RNA 3 of Cucumber mosaic virus J Gen Virol 85 3757–3763 Occurrence Handle15557249 Occurrence Handle10.1099/vir.0.80345-0 Occurrence Handle1:CAS:528:DC%2BD2cXhtVOmsbjE

    Article  PubMed  CAS  Google Scholar 

  • ME Mawassi ME Mietkiewska L Hilf A Ashoulin V Karasev R Gafny RF Lee SM Garnsey WO Dawson M Bar-Joseph (1995) ArticleTitleMultiple species of defective RNAs in plants infected with citrus tristeza virus Virology 214 264–268 Occurrence Handle8525626 Occurrence Handle10.1006/viro.1995.9930 Occurrence Handle1:CAS:528:DyaK2MXpslOmtbk%3D

    Article  PubMed  CAS  Google Scholar 

  • P Moreno J Guerri N Muñoz (1990) ArticleTitleIdentification of Spanish strains of Citrus tristeza virus (CTV) by analysis of double-stranded RNAs (dsRNA) Phytopathology 80 477–482 Occurrence Handle1:CAS:528:DyaK3cXkslCis7Y%3D

    CAS  Google Scholar 

  • PD Nagy AE Simon (1997) ArticleTitleNew insights into the mechanisms of RNA recombination Virology 235 1–9 Occurrence Handle9300032 Occurrence Handle10.1006/viro.1997.8681 Occurrence Handle1:CAS:528:DyaK2sXlsFGjtrs%3D

    Article  PubMed  CAS  Google Scholar 

  • P Palukaitis F García-Arenal (2003) ArticleTitleCucumoviruses Adv Virus Res 62 241–323 Occurrence Handle14719367 Occurrence Handle1:CAS:528:DC%2BD2cXhvF2ktL8%3D

    PubMed  CAS  Google Scholar 

  • P Palukaitis MJ Roossinck RG Dietzgen RIB Francki (1992) ArticleTitleCucumber mosaic virus Adv Virus Res 41 281–348 Occurrence Handle1575085 Occurrence Handle1:CAS:528:DyaK3sXjsVGn

    PubMed  CAS  Google Scholar 

  • P Palukaitis M Zaitlin (1997) ArticleTitleReplicase-mediated resistance to plant virus disease Adv Virus Res 48 349–377 Occurrence Handle9233437 Occurrence Handle1:CAS:528:DyaK2sXntVeis7k%3D

    PubMed  CAS  Google Scholar 

  • RO Resende P de Haan E van de Vossen AC de Avila R Goldbach D Peters (1992) ArticleTitleDefective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions J Gen Virol 73 2509–2516 Occurrence Handle1:CAS:528:DyaK38XmsVKntL4%3D

    CAS  Google Scholar 

  • J Romero Q Huang J Pogany JJ Bujarski (1993) ArticleTitleCharacterization of defective interfering RNA components that increase symptom severity of broad bean mottle virus infections Virology 194 576–584 Occurrence Handle8503176 Occurrence Handle10.1006/viro.1993.1297 Occurrence Handle1:CAS:528:DyaK3sXks1Omt7o%3D

    Article  PubMed  CAS  Google Scholar 

  • ML Roossinck (2001) ArticleTitleCucumber mosaic virus, a model for RNA virus evolution Mol Plant Pathol 2 59–63 Occurrence Handle10.1046/j.1364-3703.2001.00058.x Occurrence Handle1:CAS:528:DC%2BD3MXltVKksrs%3D Occurrence Handle20572992

    Article  CAS  PubMed  Google Scholar 

  • Roossinck MJ, Bujarski J, Ding SW, Hajimorad R, Hanada K, Scott S, Tousignant M (2000) Bromoviridae. In: van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (eds) Virus taxonomy: classification and nomenclature of viruses. Seventh Report of the International Committee on the Taxonomy of Viruses, Academic Press, San Diego, pp. 923–935

  • MJ Roossinck L Zhang KH Hellwald (1999) ArticleTitleRearrangements in the 5′ nontranslated region and phylogenetic analyzes of cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups J Virol 73 6752–6758 Occurrence Handle10400773 Occurrence Handle1:CAS:528:DyaK1MXksFKnurs%3D

    PubMed  CAS  Google Scholar 

  • L Roux AE Simon JJ Holland (1991) ArticleTitleEffect of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo Adv Virus Res 40 181–211 Occurrence Handle1957718 Occurrence Handle1:CAS:528:DyaK38XnvF2gsA%3D%3D Occurrence Handle10.1016/S0065-3527(08)60279-1

    Article  PubMed  CAS  Google Scholar 

  • L Rubio HH Yeh T Tian BW Falk (2000) ArticleTitleA heterogeneous population of defective RNAs is associated with Lettuce infectious yellows virus (LIYV) Virology 271 205–212 Occurrence Handle10814585 Occurrence Handle10.1006/viro.2000.0318 Occurrence Handle1:CAS:528:DC%2BD3cXjt1Sju7o%3D

    Article  PubMed  CAS  Google Scholar 

  • KBG Scholthof HB Scholthof AO Jackson (1995) ArticleTitleThe effect of defective interfering RNAs on the accumulation of tomato bushy stunt virus proteins and implications for disease attenuation Virology 211 324–328 Occurrence Handle7645230 Occurrence Handle10.1006/viro.1995.1410 Occurrence Handle1:CAS:528:DyaK2MXntlWjsbk%3D

    Article  PubMed  CAS  Google Scholar 

  • AE Simon MJ Roossinck Z Havelda (2004) ArticleTitlePlant virus satellite and defective interfering RNAs: new paradigms for a new century Annu Rev Phytopathol 42 415–437 Occurrence Handle15283672 Occurrence Handle10.1146/annurev.phyto.42.040803.140402 Occurrence Handle1:CAS:528:DC%2BD2cXotFyrur0%3D

    Article  PubMed  CAS  Google Scholar 

  • AJ Soards AM Murphy P Palukaitis JP Carr (2002) ArticleTitleVirulence and differential local and systemic spread of cucumber mosaic virus in tobacco are affected by the CMV 2b protein Mol Plant Microbe Interact 15 647–653 Occurrence Handle12118880 Occurrence Handle1:CAS:528:DC%2BD38Xlt1Wkurs%3D

    PubMed  CAS  Google Scholar 

  • KA White JB Bancroft GA Mackie (1991) ArticleTitleDefective RNAs of clover yellow mosaic virus encode non-structural/coat protein fusion products Virology 183 479–486 Occurrence Handle1830181 Occurrence Handle10.1016/0042-6822(91)90977-J Occurrence Handle1:CAS:528:DyaK38XhvVykurs%3D

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universidad Politécnica de Valencia (COMAV-UPV), Valencia, Spain

    C. López & F. Nuez

  2. IRTA Ctra. de Cabrils km 2, Cabrils, Barcelona, Spain

    J. Aramburu & L. Galipienso

Authors
  1. C. López
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  2. J. Aramburu
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  3. L. Galipienso
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  4. F. Nuez
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López, C., Aramburu, J., Galipienso, L. et al. Characterisation of several heterogeneous species of defective RNAs derived from RNA 3 of cucumber mosaic virus. Arch Virol 152, 621–627 (2007). https://doi.org/10.1007/s00705-006-0875-z

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  • DOI: https://doi.org/10.1007/s00705-006-0875-z

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