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The major population of PHA-stimulated PBMC infected by R5 or X4 HIV variants after a single cycle of infection is predominantly composed of CD45RO+CD4+ T lymphocytes

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Summary.

PHA-stimulated peripheral blood mononuclear cells (PBMCs) are widely used for investigating replication and neutralization of HIV primary isolates in vitro. The objective of this study was to identify the T lymphocyte subset(s) that are found infected after one replication cycle by either R5- or X4-HIV-1 variants in PHA-stimulated PBMCs from healthy donors. Infected T lymphocytes were detected by intracellular p24 staining and characterized by cell surface immunophenotyping using flow cytometry. The predominant lymphocyte subset expressing p24 after 24 h of infection with either R5 or X4 HIV-1 strains was found to exhibit mainly the memory CD45RO phenotype, a greater percentage of CD62L+CD45RO+ central memory T lymphocytes was infected with X4 HIV strains. Although some CD45RA+ lymphocytes were also infected, these cells co-expressed CD45RO+. The proportion of lymphocytes expressing CD4 and CD4/CD45RO decreased by 20% after 24 h of infection. A 2-fold decrease of CD4+CD8+ T lymphocytes could also be recorded, even though this subset accounted for less than 5% of total lymphocytes in control cultures. Moreover, CD4+CD8+ T cells further decreased by 90% after 4 days of infection, a time at which they scored p24+. Therefore, our results indicate that the in vitro infection system of PHA-stimulated PBMC utilized in neutralization assays provides an appropriate model for the study of infected CD45RO+ lymphocytes but not CD45RA+ lymphocytes.

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  1. Institut de Virologie, EA 3770, Université Louis Pasteur, Strasbourg, France

    V. Holl, S. Schmidt, A.-M. Aubertin & C. Moog

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  1. V. Holl
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  3. A.-M. Aubertin
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Holl, V., Schmidt, S., Aubertin, AM. et al. The major population of PHA-stimulated PBMC infected by R5 or X4 HIV variants after a single cycle of infection is predominantly composed of CD45RO+CD4+ T lymphocytes. Arch Virol 152, 507–518 (2007). https://doi.org/10.1007/s00705-006-0873-1

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