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The role of the C-terminal region of olive latent virus 1 coat protein in host systemic infection

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Summary.

A full-length cDNA clone of olive latent virus 1 (OLV-1), a member of the genus Necrovirus, family Tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. A mutant clone, denoted Δ3297, was obtained by deleting the nucleotide in position 3297, thus inducing a frameshift and replacing the last 49 amino acids of the viral coat protein (CP) by a shorter sequence of 39 amino acids. This mutant was viable, stable, able to synthesize a smaller CP, and able to give rise to the formation of apparently intact virus particles. Cell-to-cell movement of Δ3297 in Nicotiana benthamiana leaves was not affected, but, contrary to wild type OLV-1, it failed to spread systemically. These results indicate that virion formation is necessary but not sufficient for long-distance movement for OLV-1 and highlights the role of the CP carboxy-terminal domain in systemic infection.

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  1. Istituto di Virologia Vegetale del CNR, Sezione di Bari and Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi, Bari, Italy

    V. Pantaleo, F. Grieco, A. Di Franco & G. P. Martelli

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  1. V. Pantaleo
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  2. F. Grieco
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  3. A. Di Franco
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  4. G. P. Martelli
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Pantaleo, V., Grieco, F., Di Franco, A. et al. The role of the C-terminal region of olive latent virus 1 coat protein in host systemic infection. Arch Virol 151, 1973–1983 (2006). https://doi.org/10.1007/s00705-006-0767-2

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