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New temperate DNA phage BcP15 acts as a drug resistance vector

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Summary.

This study was designed to determine the role of a new temperate DNA phage BcP15 in relation to drug resistance. The multidrug resistant Shigella flexneri NK1925 was isolated from a patient of Infectious Diseases Hospital, Kolkata, India. This strain contained five plasmids ranging in size from 3 to 212 kb. After curing of five plasmids, this strain became sensitive to antibiotics. A plasmidless multidrug-resistant strain Burkholderia cepacia DR11 was isolated during the survey of microorganisms from coastal waters of deltaic Sunderbans. This strain always released a temperate phage BcP15 into culture supernatant. Turbid plaque formation was observed on the lawn of a plasmidless version (Pl−35) of Shigella flexneri NK1925. A few distinct clones (Pl−35R) appeared within the region of each plaque after 18 h incubation. S. flexneri NK1925, Pl−35, and Pl−35R clones showed the same PFGE band pattern of XbaI-digested chromosomal DNA. However, Pl−35R clones were resistant to co-trimoxazole, trimethoprim, and eryth- romycin, to which B. cepacia DR11 was also resistant. Southern hybridization results indicated that these three antibiotic resistances in Pl−35R clones were due to a BcP15 phage lysogen in the Pl−35 version of S. flexneri NK1925.

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Authors and Affiliations

  1. Sonamukhi College, Sonamukhi, Bankura, India

    D. K. Hens

  2. Department of Botany, Burdwan University, Burdwan, India

    N. C. Chatterjee

  3. National Institute of Cholera and Enteric Diseases, Beliaghata, India

    R. Kumar

Authors
  1. D. K. Hens
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  2. N. C. Chatterjee
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  3. R. Kumar
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Hens, D., Chatterjee, N. & Kumar, R. New temperate DNA phage BcP15 acts as a drug resistance vector. Arch Virol 151, 1345–1353 (2006). https://doi.org/10.1007/s00705-005-0713-8

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  • DOI: https://doi.org/10.1007/s00705-005-0713-8

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