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Capsid-targeted viral inactivation can destroy dengue 2 virus from within in vitro

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Summary.

Capsid-targeted viral inactivation (CTVI) has emerged as a conceptually powerful antiviral strategy that exploits viral structural proteins to target a destructive enzyme specifically into progeny virions. We have recently demonstrated the principle of CTVI against dengue virus infection and observed a modest therapeutic effect in vitro (Arch Virol 2005, 150: 659–669). Here we tested a prophylactic model of CTVI, in which mammalian cells stably expressing the dengue 2 virus capsid protein fused to a nuclease were infected with dengue virus and determined the effects on progeny virion infectivity. CTVI efficiently destroyed dengue 2 virus from within and decreased the infectious titers by 103- to 104-fold, suggesting that CTVI has potential in the prophylactic application for dengue virus infection.

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Authors and Affiliations

  1. State Key Laboratory of Pathogen and Biosecurity, Beijing, P.R. China

    C.-F. Qin & E-D. Qin

  2. Laboratory of Virology, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, P.R. China

    C.-F. Qin & E-D. Qin

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  1. C.-F. Qin
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  2. E-D. Qin
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Qin, CF., Qin, ED. Capsid-targeted viral inactivation can destroy dengue 2 virus from within in vitro. Arch Virol 151, 379–385 (2006). https://doi.org/10.1007/s00705-005-0631-9

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  • DOI: https://doi.org/10.1007/s00705-005-0631-9

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