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Genome characterization of a flexuous rod-shaped mycovirus, Botrytis virus X, reveals high amino acid identity to genes from plant ‘potex-like’ viruses

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Summary.

This study reports the molecular characterization of a flexuous rod-shaped mycovirus, Botrytis virus X (BVX), infecting the plant-pathogenic fungus, Botrytis cinerea. BVX contains a ssRNA genome of 6966 nucleotides, and a poly(A) tract at or very near the 3′ terminus. Computer analysis of the genomic cDNA sequence of BVX revealed five potential open reading frames (ORFs). ORF1 showed significant amino acid sequence identity to the replicase proteins of plant ‘potex-like’ viruses, including 73% identity to the RNA-dependent RNA polymerase (RdRp) region of the allexivirus, garlic virus A (GarV-A). The C-terminal region of ORF3 shared amino acid homology with plant ‘potex-like’ coat proteins. The remaining ORFs did not reveal significant homology with known protein sequences. BVX differs substantially from Botrytis virus F (BVF), another flexuous rod-shaped mycovirus characterized from the same B. Cinerea isolate. It is proposed that the mycovirus BVX belongs to a new, as yet unassigned genus in the plant ‘potex-like’ virus group, distinct from BVF.

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References

  • Ballance DJ (1990) Transformation systems for filamentous fungi and an overview of fungal gene structure. In: Long SA, Berka RM (eds) Molecular industrial mycology: systems for filamentous fungi, vol. 8. Dekker, New York, pp 1–29

  • Beever RE, Weeds PL (2004) Taxonomy and genetic variation of Botrytis and Botryotinia. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology, and control. Kluwer, Dordrecht, pp 29–52

  • Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L, Zurcher EJ (eds) (1996) Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20th August 1996. URL: http://image.fs.uidaho.edu/vide/refs.htm

  • Buck KW (1986) Fungal virology: an overview. In: Buck KW (ed) Fungal virology. CRC Press, Boca Raton, FL, pp 1–84

  • A Callaway D Giesman-Cookmeyer ET Gillock TL Sit SA Lommel (2001) ArticleTitleThe multifunctional capsid proteins of plant RNA viruses. Annu Rev Phytopathol 39 419–460 Occurrence Handle10.1146/annurev.phyto.39.1.419 Occurrence Handle1:CAS:528:DC%2BD3MXmvVygsb0%3D Occurrence Handle11701872

    Article  CAS  PubMed  Google Scholar 

  • M Castro K Kramer L Valdivia S Ortiz J Benavente A Castillo (1999) ArticleTitleA new double-stranded RNA mycovirus from Botrytis cinerea. FEMS Microbiol Lett 175 95–99 Occurrence Handle1:CAS:528:DyaK1MXjslegtbw%3D Occurrence Handle10361713

    CAS  PubMed  Google Scholar 

  • M Castro K Kramer L Valdivia S Ortiz A Castillo (2003) ArticleTitleA double-stranded RNA mycovirus confers hypovirulence-associated traits to Botrytis cinerea. FEMS Microbiol Lett 228 87–91 Occurrence Handle10.1016/S0378-1097(03)00755-9 Occurrence Handle1:CAS:528:DC%2BD3sXovVegtb4%3D Occurrence Handle14612241

    Article  CAS  PubMed  Google Scholar 

  • Coley-Smith JR, Verhoeff K, Jarvis WR (1980) The biology of botrytis. Academic Press, London

  • J Devereux P Haeberli O Smithies (1984) ArticleTitleA comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12 387–395 Occurrence Handle1:CAS:528:DyaL2cXot1eqtA%3D%3D Occurrence Handle6546423

    CAS  PubMed  Google Scholar 

  • VV Dolja VP Boyko AA Agranovsky EV Koonin (1991) ArticleTitlePhylogeny of capsid proteins of rod-shaped and filamentous RNA plant viruses: two families with distinct patterns of sequence and probably structure conservation. Virology 184 79–86 Occurrence Handle1:STN:280:By6A3srnvFU%3D Occurrence Handle1871982

    CAS  PubMed  Google Scholar 

  • J Felsenstein (1985) ArticleTitleConfidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791

    Google Scholar 

  • Felsenstein J (1995) PHYLIP version 3.5, University of Washington, Seattle

  • SA Ghabrial (1998) ArticleTitleOrigin, adaptation and evolutionary pathways of fungal viruses. Virus Genes 16 119–131 Occurrence Handle10.1023/A:1007966229595 Occurrence Handle1:CAS:528:DyaK1cXitFOjt7Y%3D Occurrence Handle9562896

    Article  CAS  PubMed  Google Scholar 

  • Gibbs A, Keese PK (1994) In search of the origins of viral genes. In: Gibbs AJ, Calisher CH, Garcia-Arnel F (eds) Molecular basis of viral evolution. Cambridge University Press, Cambridge, pp 76–90

  • AE Gorbalenya EV Koonin (1989) ArticleTitleViral proteins containing the purine-NTP sequence pattern. Nucleic Acids Res 17 8413–8440 Occurrence Handle1:CAS:528:DyaK3cXjtlSrsw%3D%3D Occurrence Handle2555771

    CAS  PubMed  Google Scholar 

  • RLJ Howitt RE Beever MN Pearson RLS Forster (1995) ArticleTitlePresence of double-stranded RNA and virus-like particles in Botrytis cinerea. Mycol Res 99 1472–1478 Occurrence Handle1:CAS:528:DyaK28Xht1Crtbo%3D

    CAS  Google Scholar 

  • RLJ Howitt RE Beever MN Pearson RLS Forster (2001) ArticleTitleGenome characterization of Botrytis virus F, a flexuous rod-shaped mycovirus resembling plant ‘potex-like’ viruses. J Gen Virol 82 67–78 Occurrence Handle1:CAS:528:DC%2BD3MXjtVGhtA%3D%3D Occurrence Handle11125160

    CAS  PubMed  Google Scholar 

  • EV Koonin (1991) ArticleTitleThe phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol 72 2197–2206 Occurrence Handle1895057

    PubMed  Google Scholar 

  • EV Koonin VV Dolja (1993) ArticleTitleEvolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28 375–430 Occurrence Handle1:CAS:528:DyaK3sXms1KjsL0%3D Occurrence Handle8269709

    CAS  PubMed  Google Scholar 

  • JR Marienfeld M Unseld P Brandt A Brennicke (1997) ArticleTitleViral nucleic acid sequence transfer between fungi and plants. Trends Genet 13 260–261 Occurrence Handle10.1016/S0168-9525(97)01168-2 Occurrence Handle1:CAS:528:DyaK2sXksFOgtLc%3D Occurrence Handle9242047

    Article  CAS  PubMed  Google Scholar 

  • SY Morozov KV Kanyuka KE Levay SK Zavriev (1990) ArticleTitleThe putative RNA replicase of potato virus M: obvious sequence similarity with potex- and tymoviruses. Virology 179 911–914 Occurrence Handle10.1016/0042-6822(90)90167-P Occurrence Handle1:CAS:528:DyaK3MXitVWhtQ%3D%3D Occurrence Handle2238481

    Article  CAS  PubMed  Google Scholar 

  • PA Revill AD Davidson PJ Wright (1994) ArticleTitleThe nucleotide sequence and genome organisation of mushroom bacilliform virus: a single-stranded RNA virus of Agaricus bisporus (Lange) Imbach. Virology 202 904–911 Occurrence Handle10.1006/viro.1994.1412 Occurrence Handle1:CAS:528:DyaK2MXjtlyisA%3D%3D Occurrence Handle8030251

    Article  CAS  PubMed  Google Scholar 

  • MN Rozanov EV Koonin AE Gorbalenya (1992) ArticleTitleConservation of the putative methyltransferase domain: a hallmark of the ‘Sindbis-like’ supergroup of positive-strand RNA viruses. J Gen Virol 73 2129–2134 Occurrence Handle1:CAS:528:DyaK38XlsVKrtbw%3D Occurrence Handle1645151

    CAS  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

  • TL Sit KA White S Holy U Padmanabhan M Eweida M Hiebert GA Mackie MG Abouhaidar (1990) ArticleTitleComplete nucleotide sequence of clover yellow mosaic virus RNA. J Gen Virol 71 1913–1920 Occurrence Handle1:CAS:528:DyaK3MXhsFOrsbg%3D Occurrence Handle2212986

    CAS  PubMed  Google Scholar 

  • JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 Occurrence Handle10.1093/nar/25.24.4876 Occurrence Handle1:CAS:528:DyaK1cXntFyntQ%3D%3D Occurrence Handle9396791

    Article  CAS  PubMed  Google Scholar 

  • S Vilches A Castillo (1997) ArticleTitleA double-stranded RNA mycovirus in Botrytis cinerea. FEMS Microbiol Lett 155 125–130 Occurrence Handle1:CAS:528:DyaK2sXmtFKntb4%3D Occurrence Handle9345772

    CAS  PubMed  Google Scholar 

  • HJ Vogel (1964) ArticleTitleDistribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98 435–446 Occurrence Handle10.1086/282338 Occurrence Handle1:CAS:528:DyaF2MXmvVChsg%3D%3D

    Article  CAS  Google Scholar 

  • Wostemeyer J, Wostemeyer A, Voigt K (1997) Horizontal gene transfer in the rhizosphere: a curiosity or a driving force in evolution? In: Tommerup IC, Andrews JH (eds) Advances in botanical research, vol 24. Academic Press, London, pp 399–429

  • T Yokoi Y Takemoto M Suzuki S Yamashita T Hibi (1999) ArticleTitleThe nucleotide sequence and genome organization of Sclerophthora macrospora virus B. Virology 264 344–349 Occurrence Handle10.1006/viro.1999.0018 Occurrence Handle1:CAS:528:DyaK1MXnt1OrtLo%3D Occurrence Handle10562496

    Article  CAS  PubMed  Google Scholar 

  • T Yokoi S Yamashita T Hibi (2003) ArticleTitleThe nucleotide sequence and genome organization of Sclerophthora macrospora virus A. Virology 311 394–399 Occurrence Handle10.1016/S0042-6822(03)00183-1 Occurrence Handle1:CAS:528:DC%2BD3sXkvFGmt7c%3D Occurrence Handle12842628

    Article  CAS  PubMed  Google Scholar 

  • HJ Yu D Lim H-S Lee (2003) ArticleTitleCharacterization of a novel single-stranded RNA mycovirus in Pleurotus ostreatus. Virology 314 9–15 Occurrence Handle10.1016/S0042-6822(03)00382-9 Occurrence Handle1:CAS:528:DC%2BD3sXnsVWru7g%3D Occurrence Handle14517055

    Article  CAS  PubMed  Google Scholar 

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Howitt, R., Beever, R., Pearson, M. et al. Genome characterization of a flexuous rod-shaped mycovirus, Botrytis virus X, reveals high amino acid identity to genes from plant ‘potex-like’ viruses. Arch Virol 151, 563–579 (2006). https://doi.org/10.1007/s00705-005-0621-y

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