Advertisement

Archives of Virology

, Volume 150, Issue 10, pp 2117–2124 | Cite as

Polymorphism of HIV-1 gag (p17) gene from female sex workers in Calcutta, India

  • S. Sengupta
  • D. Khetawat
  • S. Jana
  • K. Sarkar
  • S. K. Bhattacharya
  • S. Chakrabarti
Brief Report

Summary.

HIV-1 subtype C is the major subtype in India as evidenced from the analysis of specific regions within envelope and gag gene. The matrix protein (p17) of HIV-1 which is involved in several functions like the viral RNA transport, nuclear localization, assembly of pre-integration complex into host nucleus has been used to study the strain diversity among female sexworkers in Calcutta. The gene encoding for the HIV-1 matrix protein, p17 was amplified by nested polymerase chain reaction (PCR) from blood samples of HIV-1 seropositive female sex workers (FSW) in Calcutta, India. Genes of twenty-two samples were sequenced and the phylogenetic analysis with different global strains showed that the majority (seventeen) was clustered with Indian type C. A few samples were found to be close to other C subtypes isolated from South Africa, China and Myanmar. The comparison of Calcutta samples with the samples from other regions of India along with other non-asian subtype C sequences clearly revealed a different cluster of Indian sequences. The two samples, cal 242 and cal 709 was found to be the most divergent type and showed close relatedness with African C subtypes.

Keywords

Polymerase Chain Reaction Matrix Protein Nuclear Localization Nest Polymerase Chain Reaction Strain Diversity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boyum, A 1968Separation of leukocytes from blood and bone marrow.Scand J Clin Lab Invest217781PubMedGoogle Scholar
  2. Caumont, A, Lan, NT, Uyen, NT, Hung, PV, Schvoerer, E, Urriza, MS, Roques, P, Schrive, MH, Lien, TT, Lafon, ME, Dormont, D, Barre-Sinoussi, F, Fleury, HJ 2001Sequence analysis of env C2/V3, gag p17/p24, and pol protease regions of 25 HIV Type 1 isolates from Ho Chi Minh City, Vietnam.AIDS Res Hum Retrovir1712851291CrossRefPubMedGoogle Scholar
  3. Dupont, S, Sharova, N, DeHoratius, C, Virbasius, CM, Zhu, X, Bukrinskaya, AG, Stevenson, M, Green, MR 1999A novel nuclear export activity in HIV-1 matrix protein required for viral replication.Nature402681685CrossRefPubMedGoogle Scholar
  4. Gupta, K, Ott, D, Hope, TJ, Siliciano, RF, Boeke, JD 2000A human nuclear shuttling protein that interacts with human immunodeficiency virus type 1 matrix is packaged into virions.J Virol741181111824CrossRefPubMedGoogle Scholar
  5. Haffar, OK, Popov, S, Dubrovsky, L, Agostini, I, Tang, H, Pushkarsky, T, Nadler, SG, Bukrinsky, M 2000Two nuclear localization signals in the HIV-1 matrix protein regulate nuclear import of the HIV-1 pre-integration complex.J Mol Biol299359368CrossRefPubMedGoogle Scholar
  6. Hill, CP, Worthylake, D, Bancroft, DP, Christensen, AM, Sundquist, WI 1996Crystal structures of the trimeric HIV type 1 matrix protein: implications for membrane association and assembly.Proc Natl Acad Sci9330993104CrossRefPubMedGoogle Scholar
  7. Iversen, AK, Learn, GH, Fugger, L, Gerstoft, J, Mullins, JI, Skinhoj, P 1999Presence of multiple HIV subtypes and a high frequency of subtype chimeric viruses in heterosexually infected women.J Acquir Immune Defic Syndr22325332PubMedGoogle Scholar
  8. Jameel, S, Zafrullah, M, Ahmad, M, Kapoor, GS, Sehgal, S 1995A genetic analysis of HIV-1 from Punjab, India reveals the presence of multiple variants.AIDS9685690PubMedGoogle Scholar
  9. Kasper, P, Simmonds, P, Schneweis, KE, Kaiser, R, Matz, B, Oldenburg, J, Brackmann, HH, Holmes, EC 1995The genetic diversification of the HIV type 1 gag p17 gene in patients infected from a common source.AIDS Res Hum Retrovi1111971201Google Scholar
  10. Lole, KS, Bollinger, RC, Paranjape, RS, Gadkari, D, Kulkarni, SS, Novak, NG, Ingersoll, R, Sheppard, HW, Ray, SC 1999Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination.J Virol73152160PubMedGoogle Scholar
  11. Mandal, D, Jana, S, Bhattacharya, SK, Chakrabarti, S 2002HIV type 1 subtypes circulating in eastern and northeastern regions of India.AIDS Res Hum Retrovir1812191227CrossRefPubMedGoogle Scholar
  12. McCutchan, FE, Salminen, MO, Carr, JK, Burke, DS 1996HIV-1 genetic diversity.AIDS10S13S20Google Scholar
  13. Njai, HF, Van der Auwera, G, Ngong, CA, Heyndrickx, L, Sawadago, S, Whittle, H, Nyambi, P, Colebunders, R, van der Groen, G, Janssens, W 2004Development, evaluation, and validation of an oligonucleotide probe hybridization assay to subtype human immunodeficiency virus type 1 circulating recombinant form CRF02_AG.J Clin Microbiol4214281433CrossRefPubMedGoogle Scholar
  14. Sahni, AK, Prasad, VV, Seth, P 2002Genomic diversity of human immunodeficiency virus type-1 in India.Int J STD AIDS13115118CrossRefPubMedGoogle Scholar
  15. Sanchez-Merino V, Herrero CC, Amorin-Nink A, von Briesen H, Lopez-Galindez C, World Health Organization-UNAIDS Network for Isolation and Characterization (2003) Genetic analysis of culture-negative UNAIDS subtype C samples. AIDS Res Hum Retrovir 19(1): 49–55Google Scholar
  16. Shankarappa, R, Chatterjee, R, Learn, GH, Neogi, D, Ding, M, Roy, P, Ghosh, A, Kingsley, L, Harrison, L, Mullins, JI, Gupta, P 2001Human immunodeficiency virus type 1 env sequences from Calcutta in Eastern India: identification of features that distinguish subtype C sequences in India from other subtype C sequences.J Virol751047910487CrossRefPubMedGoogle Scholar
  17. Vitale, M, Caruso, A, De Francesco, MA, Rodella, L, Bozzo, L, Garrafa, E, Grassi, M, Gobbi, G, Cacchioli, A, Fiorentini, S 2003HIV-1 matrix protein p17 enhances the proliferative activity of natural killer cells and increases their ability to secrete proinflammatory cytokines.Br J Haematol120337343CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • S. Sengupta
    • 1
  • D. Khetawat
    • 1
  • S. Jana
    • 2
  • K. Sarkar
    • 1
  • S. K. Bhattacharya
    • 1
  • S. Chakrabarti
    • 1
  1. 1.National Institute of Cholera and Enteric Diseases, ICMRCalcuttaIndia
  2. 2.STD/HIV Intervention ProgramCalcuttaIndia

Personalised recommendations