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The protein ICP0 of herpes simplex virus type 1 is targeted to nucleoli of infected cells

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Summary.

This study describes the nucleolar localization of the viral protein ICP0 of herpes simplex virus type 1. We show that the RING finger domain of ICP0 is essential for ICP0 to localize in nucleoli of transfected and 4 hour-infected cells. ICP0 forms particular intranucleolar domains that do not correspond to any known nucleolar domains. This distribution was confirmed by immunoblots performed on fractionated infected cells. Quantitative RT-PCR experiments indicated that ICP0 did not increase the transcription from the RNA polymerase I (Pol I) promoter in transfected cells, an effect opposite to that observed on viral and cellular Pol II promoters. Nucleoli are thus, after PML bodies and centromeres, a novel nuclear structure targeted by ICP0.

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Authors and Affiliations

  1. Centre de Génétique Moléculaire et Cellulaire, UMR5534-CNRS, Equipe Silencing Viral et Remodelage de la Chromatine Université Claude Bernard Lyon 1, Villeurbanne, France

    E. Morency, P. Texier & P. Lomonte

  2. Centre de Génétique Moléculaire et Cellulaire, UMR5534-CNRS, Equipe Régulation post-transcriptionnelle de l’expression Génique Cellulaire et Virale Université Claude Bernard Lyon 1, Villeurbanne, France

    Y. Couté

  3. Centre de Génétique Moléculaire et Cellulaire, UMR5534-CNRS, Equipe Génétique Moléculaire du virus Herpes Simplex de Type 1, Université Claude Bernard Lyon 1, Villeurbanne, France

    J. Thomas

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  1. E. Morency
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  2. Y. Couté
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  3. J. Thomas
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  4. P. Texier
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  5. P. Lomonte
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E.M. and Y.C. have contributed equally to this work.

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Morency, E., Couté, Y., Thomas, J. et al. The protein ICP0 of herpes simplex virus type 1 is targeted to nucleoli of infected cells. Arch Virol 150, 2387–2395 (2005). https://doi.org/10.1007/s00705-005-0546-5

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  • DOI: https://doi.org/10.1007/s00705-005-0546-5

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