Summary.
In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.
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Hattermann, K., Müller, M., Nitsche, A. et al. Susceptibility of different eukaryotic cell lines to SARS-coronavirus. Arch Virol 150, 1023–1031 (2005). https://doi.org/10.1007/s00705-004-0461-1
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DOI: https://doi.org/10.1007/s00705-004-0461-1
Keywords
- Indirect Immunofluorescence
- Human Cell Line
- Immunofluorescence Assay
- Functional Receptor
- Susceptible Cell