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Archives of Virology

, Volume 150, Issue 2, pp 215–229 | Cite as

Detection of a novel border disease virus subgroup in Tunisian sheep

  • F. Thabti
  • C. Letellier
  • S. Hammami
  • M. Pépin
  • M. Ribière
  • A. Mesplède
  • P. Kerkhofs
  • P. Russo
Article

Summary.

Nine pestiviruses isolated from different batches of a contaminated Tunisian sheep pox vaccine and one Tunisian field ovine isolate of border disease virus (BDV) were studied at the antigenic and molecular levels. Seroneutralization tests were carried out on three vaccine isolates, the Tunisian field isolate and representative reference strains of the different pestivirus groups. The antigenic study showed that the Tunisian isolates were closer to the two BDV reference strains than to the Alfort-187 and the NADL reference strains. Comparison of the nucleotide sequences of the 5′-non coding regions of all the Tunisian isolates to those of other pestiviruses have shown that these isolates were distinct from the established pestivirus species. The entire Npro–E2 coding sequences of four Tunisian isolates were determined and compared to other pestiviruses. Segregation of these pestiviruses based on the Npro–E2 region was identical to that obtained with the 5′UTR sequences. The phylogenetic tree obtained with these sequences showed that the Tunisian isolates formed a separate branch between the BDV and CSFV groups, and consequently a possible new species within the pestivirus genus. However, as indicated by the antigenic study and the host origin of the isolates, the Tunisian isolates were assigned to a novel subgroup within the BDV species.

Keywords

Code Region Reference Strain Field Isolate Host Origin Separate Branch 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • F. Thabti
    • 1
    • 2
  • C. Letellier
    • 3
  • S. Hammami
    • 2
  • M. Pépin
    • 1
  • M. Ribière
    • 4
  • A. Mesplède
    • 3
  • P. Kerkhofs
    • 3
  • P. Russo
    • 1
  1. 1.AFSSA, Laboratoire d’Etudes et de Recherches sur les Petits Ruminants et les AbeillesSophia AntipolisFrance
  2. 2.Institut de la Recherche Vétérinaire de Tunisie, Laboratoire de VirologieTunisTunisie
  3. 3.Département de VirologieCentre d’Etudes et de Recherches Vétérinaires et AgrochimiquesBruxellesBelgique
  4. 4.AFSSA, Laboratoire d’Etudes et de Recherche Avicoles et Porcine, Unité de Virologie Immunologie PorcinesPloufraganFrance

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