Summary.
The nef genes of human and simian immunodeficiency viruses code for a membrane associated protein critical for AIDS development. SIVmac Nef presents C-terminal a 27 amino acid extension absent of HIV-1 Nef. To estimate the influence of this C-terminal domain on virus properties, we constructed viruses derived from SIVmac239 by replacing SIV nef with HIV-1 Lai nef gene (SHIV NefLai4) or with a sequence encoding a Nef fusion protein: HIV-1 Lai Nef/SIV Nef-Cterm (SHIV-Cterm). The recombinant viruses replicated efficiently in vitro in CEMx174 cells and in activated macaque PBMCs. The addition of SIV Nef C-terminal domain to HIV-1 Nef in SHIVNefLai4 did not change the in vitro properties of the chimeric virus, both viruses being more infectious than a nef deleted virus. Although the half-life of Nef fusion protein was augmented, SHIV-Cterm remained slightly less infectious than SIVmac239.
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Present address: Laboratoire de Biologie Université de Haute-Alsace, 25 rue de Herrlisheim, BP568, F-68008 Colmar cedex, France.
Received January 22, 2002; accepted May 29, 2002
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Bertsch, C., Cluet, D., Beyer, C. et al. Properties of a chimeric simian-human immunodeficiency virus expressing an hybrid HIV-1 Nef/SIVmac Nef protein . Arch Virol 147, 1963–1975 (2002). https://doi.org/10.1007/s00705-002-0857-8
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DOI: https://doi.org/10.1007/s00705-002-0857-8