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Dopaminergic mRNA expression in the intact sebstantia nigra of unilaterally 6-OHDA-lesioned and grafted rats: an in situ hybridization study

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Summary

The present study was performed to investigate the influence of intrastriatal fetal mesencephalic grafts on dopaminergic mRNA expression in the non-lesioned substantia nigra pars compacta of unilaterally 6-hydroxydopamine-lesioned rats. The expression of dopamine transporter mRNA, synaptic vesicular monoamine transporter mRNA and tyrosine hydroxylase mRNA was assessed in adjacent cryostat sections using in situ hybridization. Rotational behavior induced by apomorphine and amphetamine as well as hybridization of striatal sections cut at the grafting coordinates were used to prove the functional recovery and the presence of grafted cells, respectively. After grafting, the number of rotations was decreased and hybridization signals overlying cells in the grafted striatum were detected. Mean grain densities overlying labeled neurons in the substantia nigra pars compacta of grafted rats were compared to those of shamgrafted rats and revealed differential expression of dopamine transporter mRNA, whereas synaptic vesicular monoamine transporter mRNA and tyrosine hydroxylase mRNA expression showed no difference. The results will be discussed in relation to previous in vitro and in vivo studies suggesting a reduction of functional dopamine transporter molecules in the contralateral striatum.

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Authors and Affiliations

  1. Institute of Pharmacology and Toxicology, Medical Faculty (Charité), Humboldt University, Schumannstrasse 20/21, D-10117, Berlin, Federal Republic of Germany

    N. Drandarevski, A. Marburger, T. Reum & R. Morgenstern

  2. Molecular Neurobiology Branch, Division of Intramural Research, Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD, U.S.A.

    D. Walther & G. Uhl

  3. Departments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, U.S.A.

    G. Uhl

Authors
  1. N. Drandarevski
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  2. A. Marburger
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  3. D. Walther
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  4. T. Reum
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  5. G. Uhl
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  6. R. Morgenstern
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Drandarevski, N., Marburger, A., Walther, D. et al. Dopaminergic mRNA expression in the intact sebstantia nigra of unilaterally 6-OHDA-lesioned and grafted rats: an in situ hybridization study. J Neural Transm 108, 141–151 (2001). https://doi.org/10.1007/s007020170084

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  • DOI: https://doi.org/10.1007/s007020170084

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