Summary.
To determine whether pulmonary endothelial cells express the plasma membrane norepinephrine transporter (NET), an ovine-specific riboprobe was prepared from a partial NET cDNA clone isolated by PCR from a placental cDNA library. Northern hybridization detected a predominant 5.8 kb and a weaker transcript at 3.6 kb in RNAs isolated from near-term ovine fetal pulmonary endothelial cells. These data should be useful for future studies examining the molecular mechanisms underlying pulmonary endothelial NET expression and regulation.
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Received June 6, 1997; accepted October 30, 1997
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Tseng, YT., Padbury, J. Expression of a pulmonary endothelial norepinephrine transporter. J Neural Transm 105, 1187–1191 (1998). https://doi.org/10.1007/s007020050121
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DOI: https://doi.org/10.1007/s007020050121