Abstract
Diagnosing and treating chronic orofacial pain is challenging due to its complex structure and limited understanding of its causes and mechanisms. In this study, we used RNA sequencing to identify differentially expressed genes (DEGs) in the rostral ventral medulla (RVM) and thalamus of rats with persistent orofacial pain, aiming to explore its development. DEGs were functionally analyzed using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results showed a significant association between immune response and pain in this model. Key DEG mRNA expression trends were further validated using real-time quantitative polymerase chain reaction (RT-PCR), confirming their crucial roles in chronic orofacial pain. After injecting complete Freund’s adjuvant (CFA) into the bilateral temporomandibular joint cavity for 14 days, we observed 293 upregulated genes and 14 downregulated genes in the RVM, and 1086 upregulated genes and 37 downregulated genes in the thalamus. Furthermore, we identified 27 common DEGs with altered expression (upregulation) in both the thalamus and RVM, including Cd74, C3, Cxcl13, C1qb, Itgal, Fcgr2b, C5ar1, and Tlr2, which are pain-associated genes. Protein-protein interaction (PPI) analysis using Cytoscape revealed the involvement of Toll-like receptors, complement system, differentiation clusters, and antigen presentation-related proteins in the interaction between the thalamus and RVM. The results of this study show that the immune system seems to have a more significant influence on chronic orofacial pain. There may be direct or indirect influence between the thalamus and RVM, which may participate in the regulation of chronic orofacial pain.
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Data Availability
Raw and processed data are available from the Gene Expression Omnibus (GEO) (www.ncbi.nlm.nih.gov/geo/), series accession number: GSE222926.
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We thank all the participants who participated in the study.
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This work was supported by the CSA Clinical Research Grant (CSAA2021-05) and the CQMU Future Medicine Grant (W147).
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CY conceived and designed the study. JW collected the data. GYZ and LW participate in data analysis and interpretation. GYZ drafts the manuscript and JZ critically revises the manuscript. All authors have read and approved the final draft.
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Zhang, G., Wang, L., Wang, J. et al. RNA sequencing of the thalamus and rostral ventral medulla in rats with chronic orofacial pain. J Neural Transm (2024). https://doi.org/10.1007/s00702-024-02780-4
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DOI: https://doi.org/10.1007/s00702-024-02780-4