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Region-specific DNA alterations in focally induced seizures

  • Neurology and Preclinical Neurological Studies - Short communication
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Abstract

We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.

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Acknowledgments

This work is part of the PhD thesis of E.C.

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Authors and Affiliations

  1. Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

    Emanuela Cantafora, Giada Frenzilli, Vittoria Scarcelli, Marco Nigro & Margherita Bernardeschi

  2. Section of Neurology, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

    Filippo Sean Giorgi

  3. I.R.C.C.S. Neuromed Pozzilli, Via Roma 55, 56126, Pisa, Isernia, Italy

    Carla L. Busceti & Francesco Fornai

  4. Department of Translational Research in Medicine and Surgery, University of Pisa, Pisa, Italy

    Francesco Fornai

Authors
  1. Emanuela Cantafora
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  2. Filippo Sean Giorgi
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  3. Giada Frenzilli
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  4. Vittoria Scarcelli
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  5. Carla L. Busceti
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  6. Marco Nigro
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  7. Margherita Bernardeschi
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  8. Francesco Fornai
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Corresponding author

Correspondence to Francesco Fornai.

Additional information

E. Cantafora and F. S. Giorgi equally contributed to the present work.

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Cantafora, E., Giorgi, F.S., Frenzilli, G. et al. Region-specific DNA alterations in focally induced seizures. J Neural Transm 121, 1399–1403 (2014). https://doi.org/10.1007/s00702-014-1211-5

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  • DOI: https://doi.org/10.1007/s00702-014-1211-5

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