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Effects of atelocollagen on neural stem cell function and its migrating capacity into brain in psychiatric disease model

  • Psychiatry and Preclinical Psychiatric Studies - Original Article
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Abstract

Stem cell therapy is well proposed as a potential method for the improvement of neurodegenerative damage in the brain. Among several different procedures to reach the cells into the injured lesion, the intravenous (IV) injection has benefit as a minimally invasive approach. However, for the brain disease, prompt development of the effective treatment way of cellular biodistribution of stem cells into the brain after IV injection is needed. Atelocollagen has been used as an adjunctive material in a gene, drug and cell delivery system because of its extremely low antigenicity and bioabsorbability to protect these transplants from intrabody environment. However, there is little work about the direct effect of atelocollagen on stem cells, we examined the functional change of survival, proliferation, migration and differentiation of cultured neural stem cells (NSCs) induced by atelocollagen in vitro. By 72-h treatment 0.01–0.05 % atelocollagen showed no significant effects on survival, proliferation and migration of NSCs, while 0.03–0.05 % atelocollagen induced significant reduction of neuronal differentiation and increase of astrocytic differentiation. Furthermore, IV treated NSCs complexed with atelocollagen (0.02 %) could effectively migrate into the brain rather than NSC treated alone using chronic alcohol binge model rat. These experiments suggested that high dose of atelocollagen exerts direct influence on NSC function but under 0.03 % of atelocollagen induces beneficial effect on regenerative approach of IV administration of NSCs for CNS disease.

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Acknowledgments

This work was supported by grant-in-aid for Scientific Research No. 18390322 (Yoshinaga) and No. 18659335 (Saito) from the Japan Society for the Promotion of Science (JSPS).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Neuropsychiatry, Sapporo Medical University, School of Medicine, S-1, W-16, Chuo-ku, Sapporo, 060-8543, Japan

    Toshihiro Yoshinaga, Eri Hashimoto, Wataru Ukai, Takao Ishii, Tomohiro Shirasaka, Yoshiyasu Kigawa, Masaru Tateno, Hiroo Kaneta, Kimihiko Watanabe, Takeshi Igarashi, Seiju Kobayashi & Toshikazu Saito

  2. Department of Educational Development, Sapporo Medical University Center for Medical Education, Sapporo, Japan

    Hitoshi Sohma

  3. Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Brain Science Institute, Saitama, Japan

    Tadafumi Kato

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  1. Toshihiro Yoshinaga
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  2. Eri Hashimoto
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  3. Wataru Ukai
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  4. Takao Ishii
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  5. Tomohiro Shirasaka
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  6. Yoshiyasu Kigawa
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  8. Hiroo Kaneta
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  9. Kimihiko Watanabe
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  10. Takeshi Igarashi
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  11. Seiju Kobayashi
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  12. Hitoshi Sohma
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  13. Tadafumi Kato
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  14. Toshikazu Saito
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Corresponding author

Correspondence to Toshihiro Yoshinaga.

Additional information

Toshihiro Yoshinaga and Eri Hashimoto contributed equally to this work.

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Yoshinaga, T., Hashimoto, E., Ukai, W. et al. Effects of atelocollagen on neural stem cell function and its migrating capacity into brain in psychiatric disease model. J Neural Transm 120, 1491–1498 (2013). https://doi.org/10.1007/s00702-013-1010-4

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  • DOI: https://doi.org/10.1007/s00702-013-1010-4

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