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Differential effects of lysophospholipids on exocytosis in rat PC12 cells

  • Basic Neurosciences, Genetics and Immunology - Original Article
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Abstract

Secretory phospholipase A2 (sPLA2) activity is present in the CNS and the sPLA2-IIA isoform has been shown to induce exocytosis in cultured hippocampal neurons. However, little is known about possible contributions of various lysophospholipid species to exocytosis in neuroendocrine cells. This study was therefore carried out to examine the effects of several lysophospholipid species on exocytosis on rat pheochromocytoma-12 (PC12) cells. An increase in vesicle fusion, indicating exocytosis, was observed in PC12 cells after external infusion of lysophosphatidylinositol (LPI), but not lysophosphatidylcholine or lysophosphatidylserine by total internal reflection microscopy. Similarly, external infusion of LPI induced significant increases in capacitance, or number of spikes detected at amperometry, indicating exocytosis. Depletion of cholesterol by pre-incubation of cells with methyl beta cyclodextrin and depletion of Ca2+ by thapsigargin and incubation in zero external Ca2+ resulted in attenuation of LPI induced exocytosis, indicating that exocytosis was dependent on the integrity of lipid rafts and intracellular Ca2+. Moreover, LPI induced a rise in intracellular Ca2+ suggesting that this could be the trigger for exocytosis. It is postulated that LPI may be an active participant in sPLA2-mediated exocytosis in the CNS.

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Acknowledgments

This work was supported by grants from the National University of Singapore (R-181-000-091-112) to W.-Y. Ong and the Ministry of Education (AcRF tier 2-T206B3220) to P. Chen.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, National University of Singapore, Singapore, 119260, Singapore

    May-Thu Ma & Jin-Fei Yeo

  2. Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH, 43210, USA

    Akhlaq A. Farooqui

  3. Division of Bioengineering, Nanyang Technological University, Singapore, 639798, Singapore

    Jing Zhang & Peng Chen

  4. Department of Anatomy and Neurobiology Research Programme, National University of Singapore, Singapore, 119260, Singapore

    Wei-Yi Ong

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  1. May-Thu Ma
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Correspondence to Wei-Yi Ong.

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Ma, MT., Yeo, JF., Farooqui, A.A. et al. Differential effects of lysophospholipids on exocytosis in rat PC12 cells. J Neural Transm 117, 301–308 (2010). https://doi.org/10.1007/s00702-009-0355-1

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  • DOI: https://doi.org/10.1007/s00702-009-0355-1

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