Abstract
CacyBP/SIP was originally identified as an S100A6 (calcyclin) target and later on as a Siah-1 interacting protein. Recently, we have shown that CacyBP/SIP interacts with tubulin, which suggests its involvement in the reorganization of microtubules. In this work we examined the localization of CacyBP/SIP in cultured neurons and in brain neurons of young and aged rats, and compared this localization with that of tubulin and the tau protein. We have found that in neurons of young rats CacyBP/SIP, tubulin and tau are present in the cytoplasm and in the neuronal processes, whereas in aged animals CacyBP/SIP and tau are mainly seen in the cytoplasm of the neuronal somata. In aged rats, these changes are also accompanied by a different localization pattern of tubulin. Thus, our results show that localization of CacyBP/SIP in brain neurons is similar to that observed for tau and tubulin, which points to the involvement of CacyBP/SIP in cytoskeletal physiology.
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Acknowledgments
We thank Drs. G. Riedel and W. Leśniak for their critical reading of the manuscript. We also thank Dr. L. Sidorik for polyclonal anti-Sgt1 antibody. This work was supported by grants from the Ministry of Science and Higher Education of Poland to A. Filipek (2 P04A 01030) and G. Niewiadomska (2 PO5A 121), and by statutory funds from the Nencki Institute of Experimental Biology. G. Schneider is a recipient of a scholarship from the President of the Polish Academy of Sciences.
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702_2008_62_MOESM1_ESM.ppt
Specificity of the anti-Sgt1 antibody estimated by Western blotting. Rat brain extract (40 μg) prepared as described in Material and Methods, paragraph entitled “Tissue extracts preparation, SDS-PAGE and Western blot analysis” was loaded on the 10% SDS polyacrylamide gel, proteins were then blotted into nitrocellulose and incubated with anti-Sgt1 antibody. Then the blots were allowed to react with secondary antibodies conjugated to horseradish peroxidase (Sigma) and developed with ECL chemiluminescence kit (Amersham Biosciences) followed by exposition against an X-ray film. The positions of low molecular mass standard (BioRad) are indicated by arrows. (PPT 72 kb)
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Filipek, A., Schneider, G., Mietelska, A. et al. Age-dependent changes in neuronal distribution of CacyBP/SIP: comparison to tubulin and the tau protein. J Neural Transm 115, 1257–1264 (2008). https://doi.org/10.1007/s00702-008-0062-3
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DOI: https://doi.org/10.1007/s00702-008-0062-3