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Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines

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Summary.

Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H2O2 causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells.

Theme: Development and regeneration

Topic: Neurotrophic factors: receptors and cellular mechanisms

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Authors and Affiliations

  1. Department of Obstetrics & Gynecology, Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea

    J. Y. Min, N. W. Lee, T. Kim & H. J. Kim

  2. Department of Neurology, Korea University Medical College, 5-ga, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea

    M. H. Park, M. K. Park, K. W. Park & D. H. Lee

  3. Geriatric Health Clinic and Research Institute (GHCRI), Korea University Medical College, 516 Gojan-1-dong, Danwon-gu, Ansan-si, Gyeonggi-do, Republic of Korea

    M. H. Park & M. K. Park

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  1. J. Y. Min
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  2. M. H. Park
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  3. M. K. Park
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  4. K. W. Park
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  7. H. J. Kim
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Min, J., Park, M., Park, M. et al. Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines. J Neural Transm 113, 1821–1826 (2006). https://doi.org/10.1007/s00702-006-0500-z

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  • DOI: https://doi.org/10.1007/s00702-006-0500-z

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