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Formalin hindpaw injection induces changes in the [3H]prazosin binding to α1-adrenoceptors in specific regions of the mouse brain and spinal cord

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Summary.

Involvement of the α1-adrenoceptor subtypes in early and late phases of formalin pain was investigated by quantitative in vitro autoradiography in the spinal cord and brain structures of CD-1 mice. Total α1-adrenoceptors binding (including all α1-adrenoceptor subtypes) was assessed with [3H]prazosin; α1B-adrenoceptor was assessed with [3H]prazosin in the presence of 10 nM WB4101 to mask remaining α1-adrenoceptor subtypes. Early after formalin injection the α1-adrenoceptors (mainly α1B receptor) binding was reduced in the contralateral hind limb area of the somatosensory cortex and in the secondary motor cortex. A reduction occurred also in the ipsilateral laminae I–III of the spinal cord (both α1B- and non-α1B-adrenoceptors). Lately an increase of α1-adrenoceptors binding (mostly subtypes other than α1B) appeared in discrete amygdaloid and thalamic nuclei. These results provide the first description of changes at the level of central α1-adrenoceptors’ binding during the formalin-induced pain in mice. Their distribution suggests that they may have a functional meaning.

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Authors and Affiliations

  1. Department of Biochemistry and Department of Molecular Neuropharmacology, Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland

    I. Nalepa, J. Vetulani, M. Kowalska & B. Przewłocka

  2. C.N.R., Institute of Neuroscience, Psychobiology and Psychopharmacology, Roma, Italy

    V. Borghi & F. Pavone

Authors
  1. I. Nalepa
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  2. J. Vetulani
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  3. V. Borghi
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  4. M. Kowalska
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  5. B. Przewłocka
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  6. F. Pavone
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Nalepa, I., Vetulani, J., Borghi, V. et al. Formalin hindpaw injection induces changes in the [3H]prazosin binding to α1-adrenoceptors in specific regions of the mouse brain and spinal cord. J Neural Transm 112, 1309–1319 (2005). https://doi.org/10.1007/s00702-005-0279-3

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  • DOI: https://doi.org/10.1007/s00702-005-0279-3

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