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Dequalinium chloride inhibits the growth of human glioma cells in vitro and vivo: a study on molecular mechanism and potential targeted agents

  • ORIGINAL ARTICLE - TUMOR - GLIOMA
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Abstract

Background

Our current understanding of the role of dequalinium chloride (DECA) in the progression of glioma remains very limited. This study was aimed to investigate the effect of DECA on human glioma cell lines in vitro and vivo.

Methods

The underlying molecular mechanism was analyzed for developing potential targeted agents. MTT assay, genomic DNA electrophoresis, DAPI staining, TUNEL staining, and wound scratch assay were performed to evaluate the effect of DECA on human glioma cell lines. Bioinformatics methods were used to screen the possible signaling pathway proteins, and the expression of these proteins and the corresponding mRNA was measured.

Results

DECA significantly inhibited the growth and proliferation of human glioma cells. Screening of apoptosis-related proteins showed the mRNA expression level of 6 genes was significantly changed after DECA administration.

Conclusion

This study shows that DECA effectively inhibits the growth of glioma cells in vitro and vivo. DECA may promote glioma cell apoptosis by affecting the expression of NFKB2, HRAS, NF1, CBL, RAF1, and BCL-2 genes.

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Abbreviations

DECA:

Dequalinium chloride

TMZ:

Temozolomide

DMEM:

Dulbecco’s modified Eagle’s medium

DMSO:

Dimethyl sulfoxide

RT-PCR:

Reverse transcription polymerase chain reaction

T/C:

The relative tumor proliferation rate

RTV:

Relative tumor volume

IC50:

The median inhibitory concentration

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Funding

Open Access funding provided by Projekt DEAL. Jilin Province Health and Family Planning Commission’s Science and Technology Enhancement Plan provided financial support in the form of cash funding (NO. 2017J045). The sponsor had no role in the design or conduct of this research.

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Authors and Affiliations

  1. Department of Neurosurgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, 130021, China

    Ying Yu, Jinlu Yu, Gang Zhao & Fan Chen

  2. Department of Hematology, The second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China

    Bo Yang

Authors
  1. Ying Yu
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  2. Bo Yang
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  3. Jinlu Yu
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  4. Gang Zhao
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  5. Fan Chen
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Correspondence to Fan Chen.

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All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements) or nonfinancial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

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“All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.”

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This article is part of the Topical Collection on Tumor - Glioma

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Yu, Y., Yang, B., Yu, J. et al. Dequalinium chloride inhibits the growth of human glioma cells in vitro and vivo: a study on molecular mechanism and potential targeted agents. Acta Neurochir 162, 1683–1690 (2020). https://doi.org/10.1007/s00701-020-04401-x

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  • DOI: https://doi.org/10.1007/s00701-020-04401-x

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