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Comparison of a collagen membrane versus a fibrin sealant after a peroneal nerve section and repair: a functional and histological study

  • Experimental Research - Neurosurgical Techniques
  • Published:
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Abstract

Background

To date, fibrin sealant is considered to be one of the most effective substitutes to prevent post-operative fibrosis and to limit neuroma formation after nerve suture. Because fibrin sealant presents a number of drawbacks, more suitable techniques should be considered. The aim of this study was to functionally and histologically compare the efficiency of a fibrin sealant to a resorbable semi-permeable porcine type I collagen membrane after a peroneal nerve lesion and repair on rats.

Methods

Rats were divided into four groups: (1) a SHAM group (n = 10) in which surgery was performed without damaging the nerve, (2) a LESION group (n = 15) in which the nerve was cut and immediately sutured without additional treatment, (3) a MEMBRANE group (n = 30) in which a collagen membrane was wrapped around the lesion site, and (4) a GLUE group (n = 30) in which the peroneal nerve was coated by fibrin sealant. Peroneal Functional Index (PFI), kinematic analysis of locomotion, muscular atrophy, axonal regrowth, and irritant ranking score (IRS) were performed during three months post-surgery.

Results

Our results indicate that at the third month post-surgery, no difference in both the functional recovery and the histological measurement was observed between groups. However, no deleterious effect was observed following the use of the collagen membrane. Indeed, the porcine membrane was well-integrated into the host tissue, with no noticeable foreign body reaction at three months post-surgery.

Conclusion

Our preliminary results highlight the fact that the collagen membrane could be used as an alternative to fibrin sealant in peripheral nerve repair surgery. Indeed, animals in which the collagen membrane was used to wrap the lesion site exhibited similar functional and histological results as animals in which a fibrin sealant was used to coat the lesion. The greatest advantage of this membrane is that it could be used as a drug delivery device, regulated by its degradation rate.

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Acknowledgments

This work was supported by the Association Méditerranéenne pour le Développement des Transplantations (AMDT). Grants were provided from Aix-Marseille Université (AMU) and the Centre National de la Recherche Scientifique (CNRS). Biom’Up S.A (Saint Priest, France) provided the CovaTM membrane. The authors are grateful to Violaine SEVREZ and Numa BASILIO for their helpful assistance in Matlab® programming and statistical analysis, respectively. We also thank Biomatech S.A (Chasse-sur-Rhône, France) for their excellent help in histology and microscopy.

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Authors and Affiliations

  1. Aix-Marseille Université (AMU) and Centre National de la Recherche Scientifique (CNRS), UMR 7287 « Institut des Sciences du Mouvement (ISM) : Etienne-Jules MAREY », Equipe « Plasticité des Systèmes Nerveux et Musculaire », Parc Scientifique et Technologique de Luminy, Faculté des Sciences du Sport de Marseille, CC910 - 163 Avenue de Luminy, 13288, Marseille, Cedex 09, France

    Vincent Pertici, Jérôme Laurin, Tanguy Marqueste & Patrick Decherchi

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  1. Vincent Pertici
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  2. Jérôme Laurin
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Correspondence to Patrick Decherchi.

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Pertici, V., Laurin, J., Marqueste, T. et al. Comparison of a collagen membrane versus a fibrin sealant after a peroneal nerve section and repair: a functional and histological study. Acta Neurochir 156, 1577–1590 (2014). https://doi.org/10.1007/s00701-014-2117-6

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  • DOI: https://doi.org/10.1007/s00701-014-2117-6

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