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Expression of VEGF and collagen XVIII in meningiomas: correlations with histopathological and MRI characteristics

  • Experimental Research - Brain Tumors
  • Published:
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Abstract

Background

The objective of this study was to assess the possibility of predicting histological characteristics of meningiomas on the basis of preoperative MRI and the correlation of the expression of vascular endothelial growth factor (VEGF) and collagen XVIII with histological parameters already established as predictive of the course of these tumors.

Methods

Expression of VEGF and collagen XVIII as well as other histological characteristics was examined in meningioma tissues from 20 patients. Preoperative MRI, including dynamic imaging of contrast enhancement, was analyzed. Times to maximum enhancement and maximum intensity increase were noted from dynamic imaging. The relative intensity of the tumor in fluid-attenuated inversion recovery (FLAIR), T2-weighted and contrast enhanced T1-weighted images, as well as volumes of tumor and edema, was calculated. The edema-tumor volume ratio was defined as the edema index (EI).

Results

Both VEGF and collagen XVIII were expressed in all meningioma samples. Edema was present in 60 % of cases. The strongest correlation of VEGF expression was to EI. Among histological parameters, microvessel density (MVD) and cellularity correlated moderately with VEGF. Collagen XVIII expression correlated strongly with the maximal intensity increase after contrast agent administration (ρ = 0.71, P = 0.001) as well as with MVD and intensity of the meningioma on FLAIR images.

Conclusion

Meningiomas with faster and more intense enhancement in dynamic studies, indicative of good tumor blood supply and permeability of vasculature, are associated with high levels of collagen XVIII and VEGF expression. Occurrence of peritumoral edema in meningiomas is strongly correlated with expression of VEGF.

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Acknowledgments

We thank Pasi Ohtonen, MSc, for assistance with statistical analysis; Anna-Leena Heula, MD, for help in collecting the patient data.

Conflicts of Interest

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Authors and Affiliations

  1. Department of Neurosurgery, Institute of Clinical Medicine, University of Oulu, Oulu, P.O. Box 21, 90029 OYS, Finland

    Niina Salokorpi, Sanna Yrjänä, Esa Heikkinen & John Koivukangas

  2. Neurosurgery Research Unit, Institute of Clinical Medicine, University of Oulu, Oulu, Finland

    Niina Salokorpi, Sanna Yrjänä & John Koivukangas

  3. Department of Pathology, Institute of Clinical Medicine, University of Oulu, Oulu, Finland

    Hannu Tuominen

  4. Department of Radiology, Institute of Clinical Medicine, University of Oulu, Oulu, Finland

    Ari Karttunen

  5. Oulu Center for Cell-Matrix Research, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, University of Oulu, Oulu, Finland

    Ritva Heljasvaara & Taina Pihlajaniemi

  6. Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland

    Ritva Heljasvaara & Taina Pihlajaniemi

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  1. Niina Salokorpi
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Correspondence to Niina Salokorpi.

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Comment

The authors correlated VEGF and collagen XVIII expression with preoperative MRI parameters in a small sample of meningiomas. They found that expression of collagen XVIII was best predicted preoperatively from the maximal intensity increase seen in dynamic T1-weighted imaging and also the signal intensity on FLAIR images. They also calculated an edema index on MRI that was found to correlate with expression of VEGF but not with collagen XVIII expression. Meningiomas that displayed high intensity on T2 and FLAIR MR images, and those with faster and more intense enhancement in dynamic studies, tended to have a higher VEGF and collagen XVIII expression. The current results are interesting since they give us some clues with regard to the formation of edema in meningiomas and potentially also the growth rates. Nonetheless, the results must be seen as preliminary findings because of the small number of patients. Particularly, there were only few patients included with a higher edema index and also with high density of vascular collagen XVIII expression.

H.J. Steiger

Dusseldorf, Germany

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Salokorpi, N., Yrjänä, S., Tuominen, H. et al. Expression of VEGF and collagen XVIII in meningiomas: correlations with histopathological and MRI characteristics. Acta Neurochir 155, 989–996 (2013). https://doi.org/10.1007/s00701-013-1699-8

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  • DOI: https://doi.org/10.1007/s00701-013-1699-8

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