Summary
Background. We developed an MRI protocol to measure cerebrovascular diameter and blood flow velocity, and if we could detect cerebrovascular alterations after SAH and their impact on cerebral ischaemia.
Method. SAH was induced in 15 Wistar rats by means of the endovascular filament method; 6 other rats served as control. MRI measurements were performed on a 4.7T NMR spectrometer 1 and 48 hours after SAH and 9 days thereafter. Diffusion-weighted and T2-weighted images were acquired to detect cerebral ischaemia. The arterial spin labelling method was used to measure CBF. MR angiography was used to measure vessel diameter and blood flow velocity, from which the arterial blood flow was calculated.
Findings. The ischemic lesion volume increased between 1 and 48 hours after SAH from 0.039 to 0.26 ml (P = 0.003). CBF decreased from 53.6 to 39.1 ml/100 g/min. The vessel diameter had narrowed, the blood flow velocity diminished as did the arterial blood flow in most vessels, but only the vasoconstriction in the right proximal ICA reached significance (0.49 mm to 0.43 mm, P = 0.016). Baseline values were restored at day 9.
Conclusions. We showed that it is feasible to detect alterations of in-vivo vessel diameter and blood flow velocities and their consequences for brain damage after experimental SAH in the rat. The growth of the infarct volume between day 0 and 2 after SAH and the parallel vasoconstriction suggest that delayed cerebral ischaemia after SAH occurs in rats and that this may be caused by vasoconstriction.
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References
KJ Barry MA Gogjian BM Stein (1979) ArticleTitleSmall animal model for investigation of subarachnoid hemorrhage and cerebral vasospasm. Stroke 10 538–541 Occurrence Handle505495
JB Bederson IM Germano L Guarino (1995) ArticleTitleCortical blood flow and cerebral perfusion pressure in a new noncraniotomy model of subarachnoid hemorrhage in the rat. Stroke 26 1086–1091 Occurrence Handle7762027
JB Bederson AL Levy WH Ding R Kahn CA DiPerna AL Jenkins SuffixIII P Vallabhajosyula (1998) ArticleTitleAcute vasoconstriction after subarachnoid hemorrhage. Neurosurgery 42 352–360 Occurrence Handle10.1097/00006123-199802000-00091 Occurrence Handle9482187
RA de Graaf KP Braun K Nicolay (2001) ArticleTitleSingle-shot diffusion trace (1)H NMR spectroscopy. Magn Reson Med 45 741–748 Occurrence Handle10.1002/mrm.1101 Occurrence Handle11323799
TJ Delgado J Brismar NA Svendgaard (1985) ArticleTitleSubarachnoid haemorrhage in the rat: angiography and fluorescence microscopy of the major cerebral arteries. Stroke 16 595–602 Occurrence Handle4024174
A Germano C Imperatore D d’Avella G Costa F Tomasello (1998) ArticleTitleAntivasospastic and brain-protective effects of a hydroxyl radical scavenger (AVS) after experimental subarachnoid hemorrhage. J Neurosurg 88 1075–1081 Occurrence Handle9609303
JW Hop GJ Rinkel A Algra J van Gijn (1997) ArticleTitleCase-fatality rates and functional outcome after subarachnoid hemorrhage: a systematic review. Stroke 28 660–664 Occurrence Handle9056628
A Jackowski A Crockard G Burnstock RR Russell F Kristek (1990) ArticleTitleThe time course of intracranial pathophysiological changes following experimental subarachnoid haemorrhage in the rat. J Cereb Blood Flow Metab 10 835–849 Occurrence Handle2211877
JF Megyesi B Vollrath DA Cook JM Findlay (2000) ArticleTitleIn vivo animal models of cerebral vasospasm: a review. Neurosurgery 46 448–460 Occurrence Handle10.1097/00006123-200002000-00035 Occurrence Handle10690735
S Ono I Date M Nakajima K Onoda K Ogihara T Shiota S Asari Y Ninomiya N Yabuno T Ohmoto (1997) ArticleTitleThree-dimensional analysis of vasospastic major cerebral arteries in rats with the corrosion cast technique. Stroke 28 1631–1637 Occurrence Handle9259761
S Ono T Komuro RL Macdonald (2002) ArticleTitleHeme oxygenase-1 gene therapy for prevention of vasospasm in rats. J Neurosurg 96 1094–1102 Occurrence Handle12066912
A Piepgras C Thome P Schmiedek (1995) ArticleTitleCharacterization of an anterior circulation rat subarachnoid hemorrhage model. Stroke 26 2347–2352 Occurrence Handle7491662
GF Prunell T Mathiesen NH Diemer NA Svendgaard (2003) ArticleTitleExperimental subarachnoid hemorrhage: subarachnoid blood volume, mortality rate, neuronal death, cerebral blood flow, and perfusion pressure in three different rat models. Neurosurgery 52 165–175 Occurrence Handle10.1097/00006123-200301000-00022 Occurrence Handle12493115
AY Schwartz A Masago FA Sehba JB Bederson (2000) ArticleTitleExperimental models of subarachnoid hemorrhage in the rat: a refinement of the endovascular filament model [In Process Citation]. J Neurosci Methods 96 161–167 Occurrence Handle10.1016/S0165-0270(00)00156-4 Occurrence Handle10720681
FA Sehba WH Ding I Chereshnev JB Bederson (1999) ArticleTitleEffects of S-nitrosoglutathione on acute vasoconstriction and glutamate release after subarachnoid hemorrhage. Stroke 30 1955–1961 Occurrence Handle10471450
JA Veelken RJ Laing J Jakubowski (1995) ArticleTitleThe Sheffield model of subarachnoid hemorrhage in rats. Stroke 26 1279–1283 Occurrence Handle7604426
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van den Bergh, W., Schepers, J., Veldhuis, W. et al. Magnetic resonance imaging in experimental subarachnoid haemorrhage. Acta Neurochir (Wien) 147, 977–983 (2005). https://doi.org/10.1007/s00701-005-0539-x
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DOI: https://doi.org/10.1007/s00701-005-0539-x