Abstract
Chamaecrista oligandra from the Brazilian Cerrado, the most diverse savannah and one of 34 hot spots of the world, is herein described, illustrated, and compared with C. flexuosa var. flexuosa and C. parvistipula, its morphologically similar congeners. The flowering and fruiting, environmental preferences, distribution map, images, and preliminary conservation status of the new species are provided. Additionally, leaf anatomy and scanning electron microscopy of the leaf petiolar nectaries of the new species and the two putative closest relatives were compared. A phylogeny based on the noncoding plastidial (trnL-trnF) and nuclear ribosomal internal transcribed spacer indicated the proper placement of C. oligandra sp. nov. in Chamaecrista sect. Chamaecrista ser. Flexuosae, which is a taxonomic group that includes sub-shrubby species with underground systems conspicuously developed, leaflets with palmate-dimidiate venation, and flowers in an axillary fascicle. A key to identify species of the C. ser. Flexuosae series is provided to facilitate recognition of species that are distributed in open areas of the Brazilian Cerrado and frequently misidentified in collections.
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References
Bachman S, Moat J, Hill AW, de la Torre J, Scott B (2011) Supporting Red List threat assessments with GeoCAT: geospatial conservation assessment tool. ZooKeys 150:117–126. https://doi.org/10.3897/zookeys.150.2109
BFG. The Brazil Flora Group (2015) Growing knowledge: an overview of seed plant diversity in Brazil. Rodriguésia 66:1085–1113. https://doi.org/10.1590/2175-7860201566411
Bukatsch F (1972) Bemerkungen zur Doppelfärbung Astrablau-Safranin. Mikrokosmos 61:255
Conceição A, Queiroz LP, Lewis GP, Andrade MJG, Almeida PRM, Schnadelbach AS, Van de Berg C (2009) Phylogeny of Chamaecrista Moench (Leguminosae-Caesalpinioideae) based on nuclear and chloroplast DNA regions. Taxon 58:1168–1180
Coutinho IAC, Rando JG, Conceição AS, Meira RMSA (2016) A study of the morphoanatomical characters of the leaves of Chamaecrista (L.) Moench sect. Apoucouita (Leguminosae-Caesalpinioideae). Acta Bot Brasil 30:205–221. https://doi.org/10.1590/0102-33062016abb0035
Dantas MM, Silva MJ (2013) O gênero Chamaecrista (Leguminosae, Caesalpinioideae, Cassieae) no Parque Estadual da Serra Dourada, Goiás, Brasil. Rodriguésia 64:581–595
Darriba D, Taboada GL, Doallo R, Posada D (2014) jModelTest 2.1.5: more models, new heuristics and parallel computing. Nature Meth 9:772. https://doi.org/10.1038/nmeth.2109
Doyle JJ, Doyle JL (1987) A rapid DNA isolation method for small quantities of fresh tissues. Phytochem Bull 19:11–15
Drummond AJ, Rambaut A (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7:214. https://doi.org/10.1186/1471-2148-7-214
Drummond AJ, Suchard MA, Xie D, Rambaut A (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molec Biol Evol 29:1969–1973. https://doi.org/10.1093/molbev/mss075
Farris JS, Kallersjo M, Kluge AG, Bult C (1994) Testing significance of incongruence. Cladistics 10:315–319
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
Fortuna-Perez AP, Silva MJ, Cândido ES, Vargas W, Monteiro TC, Vatanparast M (2017) Eriosema elegans (Leguminosae, Papilionoideae): a new species from the Highlands of Goiás State, Brazil. Phytotaxa 296:81–87. https://doi.org/10.11646/phytotaxa.296.1.6
Francino DMT, Sant’Anna-Santos BF, Silva KLF, Thadeo M, Meira RMSA, Azevedo AA (2006) Anatomia foliar e caulinar de Chamaecrista trichopoda (Caesalpinioideae) e histoquímica do nectário extrafloral. Pl Danin 24:695–705
Francino DMT, Coutinho IAC, Dalvi VC, Azevedo AA, Conceição AS, Meira RMSA (2015) Anatomical interpretations of the taxonomy of Chamaecrista section Absus (Leguminosae - Caesalpinioideae s.l.). Pl Syst Evol 301:2087–2103. https://doi.org/10.1590/S0100-83582006000400010
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98
Irwin HS, Barneby RC (1982) The American Cassiinae: a synoptical revision of Leguminosae tribe Cassieae subtribe Cassiinae in the New World. Mem New York Bot Gard 35:455–918
IUCN (2016) Guidelines for using the IUCN red list categories and criteria. Version 13. Prepared by the Standards and Petitions Subcommittee of the IUCN Species Survival Commission. IUCN, Gland and Cambridge. http://cmsdocs.s3.amazonaws.com/RedListGuidelines.pdf
Lewis GP, Schrire B, Mackinder B, Lock M (2005) Legumes of the world. Royal Botanic Gardens, Kew
LPWG. The Legume Phylogeny Working Group (2017) A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny. Taxon 66:44–77. https://doi.org/10.12705/661.3
QGIS. Quantum GIS Development Team (2015) Quantum GIS geographic information system. Version 2.8.2
Rando JG, Pirani JR (2012) A new species of Chamaecrista sect. Chamaecrista ser. Flexuosae (Leguminosae, Caesalpinioideae) from Serra do Cipó, Minas Gerais, Brazil. Brittonia 64:241–245
Rando JG, Zuntini AR, Conceição AS, van den Berg C, Pirani JR, Queiroz LP (2016) Phylogeny of Chamaecrista ser. Coriaceae (Leguminosae) unveils a lineage recently diversified in Brazilian Campo Rupestre vegetation. Int J Pl Sci 177:3–17. https://doi.org/10.1111/j.1469-8137.2006.01902.x
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Silva MJ (2014) Manihot veadeirensis (Euphorbiaceae s.s.): a new species from the Brazilian Cerrado. Syst Bot 39:1161–1165. https://doi.org/10.1600/036364414X682625
Silva MJ, Antunes LLC (2015) An update of the Brazilian species of Aeschynomene sect. Ochopodium ser. Viscidulae including a new species and a new synonym. Phytotaxa 184:31–38. https://doi.org/10.11646/phytotaxa.184.1.4
Silva MJ, Souza AO (2014) A new species of the genus Chamaecrista (Leguminosae, Caesalpinioideae) from Chapada dos Veadeiros, Goiás, Brazil. Phytotaxa 174:181–186. https://doi.org/10.11646/phytotaxa.174.3.7
Souza AO, Silva MJ (2015a) Estudo taxonômico do gênero Chamaecrista Moench. (Caesalpinioideae, Leguminosae) na Floresta Nacional de Silvânia, Goiás, Brasil. Iheringia, Bot. 70:73–88
Souza AO, Silva MJ (2015b) Novelties in Chamaecrista (Fabaceae, Caesalpinioideae) from Brazilian Savannah. Phytotaxa 239:143–154. https://doi.org/10.11646/phytotaxa.239.2.3
Souza AO, Silva MJ (2018) Chamaecrista elata (Leguminosae, Caesalpinioideae), a new species from the highlands of Goiás, Brazil. Brittonia 70:241–247. https://doi.org/10.1007/s12228-017-9515-z
Sun Y, Skinner DZ, Liang GH, Hulbert SH (1994) Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA. Theor Appl Genet 89:26–32. https://doi.org/10.1007/BF00226978
Swofford DL (2003) PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0. Sinauer, Sunderland
Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Pl Molec Biol 15:1105–1109. https://doi.org/10.1007/BF00037152
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molec Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/mst197
Thiers B (continuously updated) Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium, New York. Available at: http://sweetgum.nybg.org/ih/. Accessed 26 Mar 2018
Torres DC, Matos JP, Lima S, Fernandes AG, Nunes EP, Grangeiro TB (2011) Phylogenetic relationships within Chamaecrista sect. Xerocalyx (Leguminosae, Caesalpinioideae) inferred from the cpDNA trnE-trnT intergenic spacer and nrDNA ITS sequences. Genet Molec Biol 34:244–251. https://doi.org/10.1590/S1415-47572011000200014
Acknowledgements
The first author thanks Renato Galhardo for the illustrations and the LABMIC team (Multiuser Laboratory for High-Resolution Microscopy, at the Federal University of Goiás).
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da Silva, M.J., de Souza, A.O. & Alonso, A.A. A new species for the legume genus Chamaecrista (Fabaceae, Caesalpinioideae) supported by molecular, morphological, and anatomical data. Plant Syst Evol 305, 325–340 (2019). https://doi.org/10.1007/s00606-019-01569-3
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DOI: https://doi.org/10.1007/s00606-019-01569-3