Plant Systematics and Evolution

, Volume 304, Issue 5, pp 583–606 | Cite as

Karyological studies of Iranian Allium L. (Amaryllidaceae) species with focus on sect. Acanthoprason. 1. Mitotic chromosomes

  • Alireza Dolatyari
  • Shahryar Saeidi Mehrvarz
  • Seyed Abolhassan Shahzadeh Fazeli
  • Mohammad Reza Naghavi
  • Reinhard M. Fritsch
Original Article
  • 83 Downloads

Abstract

Eighteen species and subspecies (34 accessions) of Allium sect. Acanthoprason and 11 species (17 accessions) belonging to other subgenera and sections of Allium were karyologically investigated and include first reports for 12 species. The examined plants of 47 accessions were diploid, three accessions of two species were tetraploid, and in the A. bisotunense accession, we found a mix of di- and triploid individuals. B chromosomes were found in 10 accessions. A basic chromosome number of x = 8 was confirmed for all investigated members of subg. Melanocrommyum and subg. Allium, and x = 9 for Allium tripedale of subg. Nectaroscordum. Idiograms were drawn for each accession, and metaphase images are presented illustrating observed chromosomal variations. Also, karyotype features and asymmetry parameters were calculated for all accessions. Chromosomal aberrations, e.g. aneuploid cells or loss of whole or parts of chromosome arms, were rarely observed. In general, the karyotypes showed low variation in inter- and intrachromosomal asymmetry especially inside of the taxonomic groups, though satellited chromosomes were good markers for subgenera and even specific for two studied sections of subg. Allium. Six different types of satellites were recognized, two of them were newly described: Type P was prevalent in subg. Melanocrommyum, and type O in sect. Codonoprasum. Statistical analyses were performed on five karyological parameters to test correct relationships and also to test previous grouping hypotheses. Although our data confirm distinct karyological characters for the subgenera investigated, the remarkable morphological diversity inside of subg. Melanocrommyum is not mirrored by striking karyological differences.

Keywords

Acanthoprason Amaryllidaceae Allium Iran Karyology Satellite 

Notes

Acknowledgements

This paper presents data from the first author’s (A. D.) PhD thesis fulfilled at Iranian biological resources center (IBRC). This organization is gratefully acknowledged for financial support. We would like to express our special thanks to Dr. H. Moazzeni and Mr. H. Ramezani for their valuable help during collecting missions. The latter mentioned person is also thanked for valuable guidance how to cultivate and root bulbs.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

606_2017_1489_MOESM1_ESM.pdf (945 kb)
Online Resource 1a–e. Arm lengths [in µm] with standard deviations (in the vertical sequence of satellites, short arms, long arms and centromere indices) in diploid members of sect. Acanthoprason (PDF 944 kb)
606_2017_1489_MOESM2_ESM.pdf (391 kb)
Online Resource 2. Arm lengths [in µm] with standard deviations (in the vertical sequence of satellites, short arms, long arms and centromere indices) in members of sect. Acanthoprason and sect. Melanocrommyum with more than 20 chromosomes (PDF 390 kb)
606_2017_1489_MOESM3_ESM.pdf (439 kb)
Online Resource 3. Arm lengths [in µm] with standard deviations (in the vertical sequence of satellites, short arms, long arms and centromere indices) in diploid members of other sections of subg. Melanocrommyum and in A. tripedale, subg. Nectaroscordum (PDF 438 kb)
606_2017_1489_MOESM4_ESM.pdf (426 kb)
Online Resource 4. Arm lengths [in µm] with standard deviations (in the vertical sequence of satellites, short arms, long arms and centromere indices) in diploid members of subg. Allium sect. Allium (PDF 425 kb)
606_2017_1489_MOESM5_ESM.pdf (311 kb)
Online Resource 5. Arm lengths [in µm] with standard deviations (in the vertical sequence of satellites, short arms, long arms and centromere indices) in A. pseudoampeloprasum, sect. Allium (PDF 310 kb)
606_2017_1489_MOESM6_ESM.pdf (362 kb)
Online Resource 6. Arm lengths [in µm] with standard deviations (in the vertical sequence of satellites, short arms, long arms and centromere indices) in A. stamineum, sect. Codonoprasum (PDF 361 kb)

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Authors and Affiliations

  1. 1.Iranian Biological Resource Centre (IBRC)ACECRTehranIran
  2. 2.Department of Biology, Faculty of SciencesUniversity of GuilanRashtIran
  3. 3.Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in BiologyUniversity of Science and CultureTehranIran
  4. 4.Department of Agronomy& Plant Breeding, Agricultural CollegeUniversity of TehranKarajIran
  5. 5.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Seeland OT GaterslebenGermany

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